Dynamics and stabilization mechanism of mitochondrial cristae morphofunction associated with turgor-driven cardiolipin biosynthesis under salt stress conditions

Abstract

Maintaining energy production efficiency is of vital importance to plants growing under changing environments. Cardiolipin localized in the inner mitochondrial membrane plays various important roles in mitochondrial function and its activity, although the regulation of mitochondrial morphology to various stress conditions remains obscure, particularly in the context of changes in cellular water relations and metabolisms. By combining single-cell metabolomics with transmission electron microscopy, we have investigated the adaptation mechanism in tomato trichome stalk cells at moderate salt stress to determine the kinetics of cellular parameters and metabolisms. We have found that turgor loss occurred just after the stress conditions, followed by the contrasting volumetric changes in mitochondria and cells, the accumulation of TCA cycle-related metabolites at osmotic adjustment, and a temporal increase in cardiolipin concentration, resulting in a reversible topological modification in the tubulo-vesicular cristae. Because all of these cellular events were dynamically observed in the same single-cells without causing any disturbance for redox states and cytoplasmic streaming, we conclude that turgor pressure might play a regulatory role in the mitochondrial morphological switch throughout the temporal activation of cardiolipin biosynthesis, which sustains mitochondrial respiration and energy conversion even under the salt stress conditions.

Applications of ambient ionization mass spectrometry in 2022: An annual review

“…Other techniques have also been demonstrated in the analysis of plant metabolites, with Walton et al using the liquid micro junction-surface sampling probe (LMJ-SSP) to study the spatial distribution of amino acids in plant material and bacterial biofilms 148 and Nakata et al using picolitre pressure-probe electrospray-ionization MS to study the effects of salt stress in single plant cells. 149 The early identification of bacterial species is of crucial importance for rapid diagnostics in order to ensure appropriate and timely treatment of infections. As such, AIMS can provide the means to characterize microbial species in near-real time.…”

“…using the liquid micro junction‐surface sampling probe (LMJ‐SSP) to study the spatial distribution of amino acids in plant material and bacterial biofilms 148 and Nakata et al. using picolitre pressure‐probe electrospray‐ionization MS to study the effects of salt stress in single plant cells 149 …”

“…using picolitre pressure‐probe electrospray‐ionization MS to study the effects of salt stress in single plant cells. 149 …”