Dynamics of apoptosis‐related gene expression during follicular development in yak

Zhang, Taojie; Basang, Wangdui; Chang, Weijie; Huo, Shengdong; Ma, Xingbin; Ju, Xingrong; Yu, Sijiu; Cui, Sheng

doi:10.1111/jpn.13527

Cited by 13 publications

(4 citation statements)

References 53 publications

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“…This result indicated that the expression of VEGFA gene in HF plays a key role in blood vessel generation to maintain the health of the follicles. Meanwhile, the expression of BAX ( 29 ), BCL2 ( 29 , 30 ), CASPASE3 ( 30 ), and CYP19A1 ( 31 ) and the concentration of E2 ( 24 ) and PROG ( 25 ) were significantly different, which is consistent with the results of prior research. All these studies illustrated that the grouping of our study was reasonable.…”

Section: Discussionsupporting

confidence: 89%

Non-targeted Metabolomics Reveals Metabolic Characteristics of Porcine Atretic Follicles

Sun

Cheng

et al. 2021

Front. Vet. Sci.

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Follicular atresia is one of the main factors limiting the reproductive power of domestic animals. At present, the molecular mechanisms involved in porcine follicular atresia at the metabolic level remain unclear. In this study, we divided the follicles of Bama Xiang pigs into healthy follicles (HFs) and atretic follicles (AFs) based on the follicle morphology. The expression of genes related to atresia in granulosa cells (GCs) and the concentration of hormones in the follicular fluid (FF) from HFs and AFs were detected. We then used liquid chromatography–mass spectrometry-based non-targeted metabolomic approach to analyze the metabolites in the FF from HFs and AFs. The results showed that the content of estradiol was significantly lower in AFs than in HFs, whereas that of progesterone was significantly higher in AFs than that in HFs. The expression of BCL2, VEGFA, and CYP19A1 was significantly higher in HFs than in AFs. In contrast, the expression of BAX and CASPASE3 was significantly lower in HFs. A total of 18 differential metabolites (DMs) were identified, including phospholipids, bioactive substances, and amino acids. The DMs were involved in 12 metabolic pathways, including arginine biosynthesis and primary bile acid biosynthesis. The levels of eight DMs were higher in the HF group than those in the AF group (p < 0.01), and those of 10 DMs were higher in the AF group than those in the HF group (p < 0.01). These findings indicate that the metabolic characteristics of porcine AFs are lower levels of lipids such as phospholipids and higher levels of amino acids and bile acids than those in HFs. Disorders of amino acid metabolism and cholic acid metabolism may contribute to porcine follicular atresia.

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Section: Discussionsupporting

confidence: 89%

Non-targeted Metabolomics Reveals Metabolic Characteristics of Porcine Atretic Follicles

Sun

Cheng

et al. 2021

Front. Vet. Sci.

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“…Therefore, we can reasonably suppose that the yak follicular fluid exosomes increase 2-OHE 2 secretion through the activation of autophagy and that this may be an effective control method by which to improve the embryo quality and pregnancy rate and have a specific effect on the biological functions of COCs. Previous studies have shown that yak follicles were classified as healthy (6–8 mm) or atretic follicles (3–6 mm) upon examination with a stereoscopic microscope [ 58 ]. Thus, in our study, we selected widely vascularized follicles larger than 8 mm in diameter with a uniform, bright, and translucent appearance.…”

Section: Discussionmentioning

confidence: 99%

Exosomes Derived from Yak Follicular Fluid Increase 2-Hydroxyestradiol Secretion by Activating Autophagy in Cumulus Cells

Wang²,

Wang³

et al. 2022

Animals

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Exosomes in the follicular fluid can carry and transfer regulatory molecules to recipient cells, thus influencing their biological functions. However, the specific effects of yak follicular fluid exosomes on 2-hydroxyestrodiol (2-OHE2) secretion remain unknown. Here, we investigated whether yak follicular fluid exosomes can increase 2-OHE2 secretion through the activation of autophagy in cumulus cells (YCCs). In vitro cultured YCCs were treated with yak follicular fluid exosomes for 6, 12, and 24 h. The effects of yak follicular fluid exosomes on autophagy and 2-OHE2 secretion were evaluated through real-time quantitative fluorescence PCR (RT-qPCR), Western blotting (WB), transfected with RFP-GFP-LC3, immunohistochemistry, and ELISA. To further investigate whether 2-OHE2 secretion was related to autophagy, YCCs were administered with yak follicular fluid exosomes, 3-methyladenine (3-MA), and rapamycin (RAPA). The results revealed that treatment with yak follicular fluid exosomes activated autophagy in YCCs and increased 2-OHE2 secretion. Conversely, the inhibition of autophagy with 3-MA blocked these effects, suggesting that autophagy has an important role in 2-OHE2 secretion in YCCs. Treatment of YCCs with rapamycin showed similar results with yak follicular fluid exosomes as there was an increase in 2-OHE2 secretion due to the activation of autophagy in the treated cumulus cells. Our results demonstrate that autophagy is enhanced by yak follicular fluid exosomes, and this is associated with an increase in 2-OHE2 secretion in YCCs.

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“…The oviduct is an important site for fertilization in yaks and previous studies focused on its morphology ( 18 ), POxia-inducible factor-1 alpha ( 19 ), ERK1/2 ( 20 ), estrogen receptor genes ( 21 ), follicle-stimulating hormone receptor (FSHR) gene ( 22 ), DNA damage induced transcript 3 ( 23 ), and apoptosis-related genes in the yak oviduct ( 24 ). There are currently no transcriptomic studies on the different parts of the yak oviduct, and there is a lack of research on the fertilization mechanism from the perspective of mRNA.…”

Section: Discussionmentioning

confidence: 99%

Identification of key genes and biological pathways in different parts of yak oviduct based on transcriptome analysis

Zhang

Zhao

et al. 2022

Front. Vet. Sci.

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The oviduct consists of three parts: the infundibulum (In), ampulla (Am), and isthmus (Is). These have the same histological structure, but different physiological functions. In this study, transcriptomics was used to analyze mRNA in these three parts of yak oviduct. The results showed that there were 325 up-regulated genes and 282 down-regulated genes in the infundibulum and ampulla. Moreover, there were 234 up-regulated genes and 776 down-regulated genes in the isthmus and ampulla, as well as 873 up-regulated genes and 297 down-regulated genes in the infundibulum and isthmus. The expression of C3 in the infundibulum was significantly higher than that in the ampulla and isthmus. The expression of FAU in the isthmus was significantly lower than that in the ampulla and infundibulum, and the expression of EEF1A1 in the ampulla was significantly higher than that in the ampulla and infundibulum. When the infundibulum was compared with the ampulla and isthmus, it was found that the up-regulated genes were enriched in the lysosome, phagosome, staphylococcus aureus infection, and leishmaniasis pathway. When the isthmus was compared with the ampulla and infundibulum, the up-regulated genes were present in the apoptosis pathway, oxidative phosphorylation, and viral myocarditis pathway. When the isthmus was compared with the infundibulum and ampulla, the down-regulated pathways were protein processing in the endoplasmic reticulum and the endocytosis. The Epstein–Barr virus infection pathway was up-regulated according to a comparison of the isthmus and infundibulum and was down-regulated based on a comparison of the isthmus and ampulla. Transcriptional misregulation in the Middle East pathway was up-regulated based on a comparison of the isthmus and ampulla and was down-regulated based on a comparison of the isthmus and infundibulum. ERBB2, JUP, CTNND1, and KRT7 were defined as the hub genes of the yak oviduct. The results of this study provide sufficient omics data for yak fertilization, which is also of great significance to altitude medicine.

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Dynamics of apoptosis‐related gene expression during follicular development in yak

Cited by 13 publications

References 53 publications

Non-targeted Metabolomics Reveals Metabolic Characteristics of Porcine Atretic Follicles

Non-targeted Metabolomics Reveals Metabolic Characteristics of Porcine Atretic Follicles

Exosomes Derived from Yak Follicular Fluid Increase 2-Hydroxyestradiol Secretion by Activating Autophagy in Cumulus Cells

Identification of key genes and biological pathways in different parts of yak oviduct based on transcriptome analysis

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