Dynamics of diesel fuel degradation in contaminated soil using organic wastes

Abstract

Bioremediation is an effective measure in dealing with such contamination, particularly those from petroleum hydrocarbon sources. The effect of soil amendments on diesel fuel degradation in soil was studied. Diesel fuel was introduced into the soil at the concentration of 5 % (w/w) and mixed with three different organic wastes tea leaf, soy cake, and potato skin, for a period of 3 months. Within 84 days, 35 % oil loss was recorded in the unamended polluted soil while 88, 81 and 75 % oil loss were recorded in the soil amended with soy cake, potato skin and tea leaf, respectively. Diesel fuel utilizing bacteria counts were significantly high in all organic wastes amended treatments, ranging from 111 9 106 to 152 9 106 colony forming unit/gram of soil, as compared to the unamended control soil which gave 31 9 106 CFU/g. The diesel fuel utilizing bacteria isolated from the oil-contaminated soil belongs to Bacillus licheniformis, Ochrobactrum tritici and Staphylococcus sp. Oil-polluted soil amended with soy cake recorded the highest oil biodegradation with a net loss of 53 %, as compared to the other treatments. Dehydrogenase enzyme activity, which was assessed by 2,3,5-triphenyltetrazolium chloride technique, correlated significantly with the total petroleum hydrocarbons degradation and accumulation of CO 2 . First-order kinetic model revealed that soy cake was the best of the three organic wastes used, with biodegradation rate constant of 0.148 day -1 and half life of 4.68 days. The results showed there is potential for soy cake, potato skin and tea leaf to enhance biodegradation of diesel in oil-contaminated soil.

Dynamics Phytoremediation of Zn and Diesel Fuel in Co-contaminated Soil using Biowastes

“…In addition, the highest rate of degradation was recorded in the soil amended with SC which is probably due to a higher amount of N and P, compared to other organic waste amendments. It was also reported by work that soil amended with SC, showed a higher rate of degradation [17,18]. Similarly, Dominguez-Rosado and Pichtel [19] recorded 67% engine oil loss in contaminated soil planted with mustard and sunflower plants.…”

Ujicoba Konsorsium Mikroba dalam Upaya Bioremediasi Tanah Tercemar Minyak Dengan Menggunakan Teknik Landfarming Skala Bangku

“…Faktor-faktor utama yang mempengaruhi adalah konsentrasi awal cemaran minyak di tanah, dosis mikroba yang diberikan, pemberian nutrisi, aerasi, serta pengaturan kelembaban dan suhu. Seringkali untuk meningkatkan aerasi dan mempertahankan kelembaban tanah ditambahkan kompos atau bahan organik sehingga proses degradasi dapat berjalan dengan efektif (10,12) . Penelitian ini dilakukan untuk menguji efektifitas pemberian dua jenis konsorsium bakteri hasil pengembangan di laboratorium, pengaruh dosis mikroba dan pengaruh pemberian bahan organik dalam menghilangkan cemaran minyak bumi di tanah dengan menggunakan teknik land farming skala bangku (bench scale).…”

Bio-Enrichment of Waste Crude Oil Polluted Soil: Amended with Bacillus 139SI and Organic Waste

“…This may increase the risk of mortality from infectious diseases [3]. A diversity of bioremediation techniques has been developed to increase the degradation rate of contaminated sites [4], [5]. More than 200 species of bacteria, yeasts, and fungi have been identified, which are capable of degrading hydrocarbons.…”