Dynamics of mobile element activity in chalcone synthase loci in the common morning glory (
            <i>Ipomoea purpurea</i>
            )

Durbin, Mary L.; Denton, Amy L.; Clegg, Michael T.

doi:10.1073/pnas.091095498

Cited by 17 publications

(12 citation statements)

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“…Along the same line, at least seventeen TEs are associated with the rice Xa21 gene cluster, which confers disease resistance against Xanthomonas oryzae [14]. In the common morning glory (Ipomoea purpurea), a remarkable variety of mobile elements reside in the chalcone synthase D locus (CHS-D), which encodes a key enzyme controlling the first committed step in the flavonoid biosynthetic pathway producing a wide range of compounds important in UV protection and defense against plant disease and herbivores [15]. Other than the above-mentioned differential retention of TEs between essential housekeeping genes and environmental response genes, Tos17 retrotransposon prefers to insert into disease/defense-related and signal transduction (kinase) genes in the rice genome [16].…”

Section: Introductionmentioning

confidence: 99%

Transposable elements are enriched within or in close proximity to xenobiotic-metabolizing cytochrome P450 genes

Chen

2007

BMC Evol Biol

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Background: Transposons, i.e. transposable elements (TEs), are the major internal spontaneous mutation agents for the variability of eukaryotic genomes. To address the general issue of whether transposons mediate genomic changes in environment-adaptation genes, we scanned two alleles per each of the six xenobiotic-metabolizing Helicoverpa zea cytochrome P450 loci, including CYP6B8, CYP6B27, CYP321A1, CYP321A2, CYP9A12v3 and CYP9A14, for the presence of transposon insertions by genome walking and sequence analysis. We also scanned thirteen Drosophila melanogaster P450s genes for TE insertions by in silico mapping and literature search.

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Section: Introductionmentioning

confidence: 99%

Transposable elements are enriched within or in close proximity to xenobiotic-metabolizing cytochrome P450 genes

Chen

2007

BMC Evol Biol

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“…Black and gray bars in c represent CHS-D and DFR-B, respectively element, Tip100 of the hAT superfamily, into the unique intron of CHS-D, which encodes chalcone synthase in I. purpurea and is required for anthocyanin pigmentation in flower limbs. One of the apparently stable a lines exhibiting white flowers is a double mutant carrying two copies of Tip100 in the CHS-D intron (Habu et al 1998;JohzukaHisatomi et al 1999;Durbin et al 2001). As expected, the incomplete-dominant flower phenotype can also be observed in the F2 segregants from an F1 hybrid between the apparently stable a and wild-type plants (Fig.…”

Section: Introductionmentioning

confidence: 54%

“…1c). Because small amounts of shorter 1.2-kb transcripts rather than normal 1.5-kb transcripts were detected in the flower buds of both a f and a mutants when the CHS-D-specific 3 0 -UTR probe was used for northern blotting Durbin et al 2001), the amounts of the CHS-D mRNAs in the flower buds of the heterozygous segregants (A rev /a f ) and the homozygous segregants (a f /a f ) in Fig. 1c are likely to be slightly overestimated values that include the residual shorter 1.2-kb transcripts.…”

Section: Accumulation Of Chs-d and Dfr-b Transcripts In The Flower Bumentioning

confidence: 99%

The molecular basis of incomplete dominance at the A locus of CHS-D in the common morning glory, Ipomoea purpurea

2010

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The mutable a(flaked (a(f)) allele at the A locus of the common morning glory (Ipomoea purpurea) confers incomplete dominance in flower pigmentation and is caused by insertion of the DNA transposon Tip100 into CHS-D, which encodes chalcone synthase and is required for anthocyanin biosynthesis. Levels of CHS-D transcripts, CHS-D protein, and anthocyanin pigment in heterozygous flowers were about half that in homozygous flowers, indicating that dosage-dependent expression of CHS-D is the primary cause of the observed incomplete dominance. This contrasts with the Nivea locus in snapdragon (Antirrhinum majus) in which incomplete dominance is caused by semi-dominant CHS alleles.

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“…The best studied mutation in the common morning glory (Ipomoea purpurea or Pharbitis purpurea) is a mutable allele of the A locus termed¯aked, also called anthocyanin¯a ked (a¯a ked or a f ) (Barker 1917;Imai and Tabuchi 1935;Kasahara 1956;Epperson and Clegg 1987;Hisatomi et al 1997;Habu et al 1998;Iida et al 1999;Durbin et al 2001;Hoshino et al 2001). In the homozygous state a f /a f , the mutable¯aked lines display white¯owers with colored¯akes (Fig.…”

Section: Introductionmentioning

confidence: 99%

The transposon Tip100 from the common morning glory is an autonomous element that can transpose in tobacco plants

et al. 2002

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The mutable flaked or a (flaked) (a(f)) line of the common morning glory (Ipomoea purpurea) displays white flowers with colored flakes, and the a(f) mutation is caused by the insertion of a transposable element named Tip100 into the CHS-D gene for anthocyanin biosynthesis. The 3.9-kb Tip100 element belongs to the Ac/Ds family and contains an ORF encoding a polypeptide of 808 amino acids. The frequency and timing of flower variegation vary in different a(f) lines, and a genetic element termed Modulator has been postulated to affect the variegation pattern. Since the pattern of flower variegation is determined by the frequency and timing of excision of Tip100 from the CHS-D gene, we wished to determine whether Tip100 is an autonomous element that is itself capable of transposition in a heterologous host. To do this, we introduced the element into the genome of tobacco plants by Agrobacterium-mediated transformation. The intact Tip100 element was able to excise from its original position in the chromosome and reinsert into new sites in the tobacco genome, whereas an internal deletion derivative was not. Based on these results, we conclude that Tip100 is an autonomous element. We also discuss the nature of the putative Modulator element affecting flower and leaf variegation in various mutable lines of the morning glory.

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Dynamics of mobile element activity in chalcone synthase loci in the common morning glory ( Ipomoea purpurea )

Cited by 17 publications

References 50 publications

Transposable elements are enriched within or in close proximity to xenobiotic-metabolizing cytochrome P450 genes

Transposable elements are enriched within or in close proximity to xenobiotic-metabolizing cytochrome P450 genes

The molecular basis of incomplete dominance at the A locus of CHS-D in the common morning glory, Ipomoea purpurea

The transposon Tip100 from the common morning glory is an autonomous element that can transpose in tobacco plants

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