Dynamics of phytoplankton pigments in water and surface sediments of a large shallow lake

Freiberg, Rene; Nõmm, Monika; Tõnno, Ilmar; Alliksaar, Tiiu; Nõges, Tiina; Kisand, Anu

doi:10.3176/earth.2011.2.03

Cited by 19 publications

(5 citation statements)

References 42 publications

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“…The increased concentrations of particulate and dissolved matters raises the respiration rate causing a decline in NEP. As the fresh sediment in Võrtsjärv still contains intact algal cells and degradation products of algal pigments (Freiberg et al, 2011), the observed increase of Chl a towards autumn is partially caused by resuspension and cannot be fully be attributed to algal growth and the reduction of zooplankton grazing pressure.…”

Section: Discussionmentioning

confidence: 99%

High-frequency metabolism study in a large and shallow temperate lake reveals seasonal switching between net autotrophy and net heterotrophy

et al. 2012

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Respiratory CO 2 release from inland waters is a major process in the global carbon cycle, retaining more than half of the carbon flux from terrestrial sources that otherwise would reach the sea. The strongly lake type-specific balance between primary production and respiration determines whether a lake acts regionally as a net sink or source of CO 2 . This study presents two-year (2009, 2010) results of high-frequency metabolism measurements in the large and shallow polymictic eutrophic Lake Võrtsjärv (area 270 km 2 ; mean depth 2.8 m). We estimated the net ecosystem production (NEP), community respiration (R) and gross primary production (GPP) from continuous measurements of oxygen, irradiance, wind and water temperature. A sinusoidal model fitted to the calculated metabolic rates showed the prevalence of net autotrophy (mean GPP:R [ 1) from early spring until August/September, whereas during the rest of the year heterotrophy (mean GPP:R \ 1) prevailed, characterizing the lake as CO 2 neutral on an annual basis. Community respiration lagged behind GPP by approximately 2 weeks, which could be explained by the bulk of the phytoplankton biomass accounted for by filamentous cyanobacteria that are considered mostly inedible to zooplankton, and the seasonally increasing role of sediment resuspension. In the warmer year 2010, the seasonal peaks of GPP, R and NEP were synchronously shifted nearly 1 month earlier compared with 2009. The strong stimulating effect of temperature on both GPP and R and its negative effect on NEP revealed by the multiple regression analysis suggests increasing metabolic rates and increasing heterotrophy in this lake type in a warmer climate.

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Section: Discussionmentioning

confidence: 99%

High-frequency metabolism study in a large and shallow temperate lake reveals seasonal switching between net autotrophy and net heterotrophy

et al. 2012

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“…The weak correlation in our study could be caused by low counting precision of this minority group but also by errors in quantification of minor pigments (such as diatoxanthin in case of chrysophytes) which are shared by several phytoplankton classes and thus may contribute to the discrepancies [ 22 ]. Diatoxanthin can also interconvert with diadinoxanthin as was shown in Freiberg et al [ 52 ] that makes the pigment-based chemotaxonomic assessment of chrysophytes and dinoflagellates more difficult. Still it is likely that a separate intercalibration exercise based on high precision counts of the minority groups might yield more consistent results and that the high sensitivity of the CHEMTAX method could prove one of its main advantages lowering the uncertainty of the minority group biomass estimates.…”

Section: Discussionmentioning

confidence: 99%

Pigment-Based Chemotaxonomy - A Quick Alternative to Determine Algal Assemblages in Large Shallow Eutrophic Lake?

et al. 2015

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Pigment-based chemotaxonomy and CHEMTAX software have proven to be a valuable phytoplankton monitoring tool in marine environments, but are yet underdeveloped to determine algal assemblages in freshwater ecosystems. The main objectives of this study were (1) to compare the results of direct microscopy and CHEMTAX in describing phytoplankton community composition dynamics in a large, shallow and eutrophic lake; (2) to analyze the efficiency of the pigment-based method to detect changes in phytoplankton seasonal dynamics and during rapid bloom periods; (3) to assess the suitability of specific marker pigments and available marker pigment:chlorophyll a ratios to follow seasonal changes in eutrophic freshwater environment. A 5-year (2009-2013) parallel phytoplankton assessment by direct microscopy and by CHEMTAX was conducted using published marker pigment:chlorophyll a ratios. Despite displaying some differences from microscopy results, the pigment-based method successfully described the overall pattern of phytoplankton community dynamics during seasonal cycle in a eutrophic lake. Good agreement between the methods was achieved for most phytoplankton groups - cyanobacteria, chlorophytes, diatoms and cryptophytes. The agreement was poor in case of chrysophytes and dinoflagellates. Our study shows clearly that published marker pigment:chlorophyll a ratios can be used to describe algal class abundances, but they need to be calibrated for specific freshwater environment. Broader use of this method would enable to expand monitoring networks and increase measurement frequencies of freshwater ecosystems to meet the goals of the Water Framework Directive.

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“…The time of extraction varied from several minutes 6,18,29 to several hours 4,7,26 and even overnight. 5,11,16,17 However, the influence on extraction efficiency of freeze-drying prior to extraction is not clear. In the case of freeze-dried sediments a 5-10 % content of water was recommended to improve extraction of polar compounds, 4,30 but some authors used acetone for extracting freeze-dried material.…”

Section: Resultsmentioning

confidence: 99%

Carotenoid determination in recent marine sediments - practical problems during sample preparation and HPLC analysis

Krajewska¹,

Szymczak‐Żyła²,

Kowalewska³

2017

10.5267/j.ccl

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An analytical procedure for the analysis of carotenoids in marine sediments rich in organic matter has been developed. Analysis of these compounds is difficult; the application of methods used by other authors required optimization for the samples studied here. The analytical procedure involved multiple ultrasound-assisted extraction with acetone followed by liquid-liquid extraction (acetone extract:benzene:water -15:1:10 v/v/v) and HPLC analysis. The influence of column temperature on pigment separation and the quantification method were investigated -a temperature of 5 °C was selected for the Lichrospher 100 RP-18e column. The pigments in the sediment extract were quantified using a method based on HPLC analysis (at 450 nm) and spectrophotometric measurements (at 450 nm), and extinction coefficients were determined for standard solutions at this wavelength. It is very important to use the value of the extinction coefficient appropriate to the wavelength at which the detection of carotenoids was carried out.

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Dynamics of phytoplankton pigments in water and surface sediments of a large shallow lake

Cited by 19 publications

References 42 publications

High-frequency metabolism study in a large and shallow temperate lake reveals seasonal switching between net autotrophy and net heterotrophy

High-frequency metabolism study in a large and shallow temperate lake reveals seasonal switching between net autotrophy and net heterotrophy

Pigment-Based Chemotaxonomy - A Quick Alternative to Determine Algal Assemblages in Large Shallow Eutrophic Lake?

Carotenoid determination in recent marine sediments - practical problems during sample preparation and HPLC analysis

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