Dynamics of sperm DNA fragmentation in domestic animals

Protamine 1 to protamine 2 ratio correlates with dynamic aspects of DNA fragmentation in human sperm

“…Because it has been further demonstrated that sperm samples from different human males can exhibit differing dynamic properties of SDF, evaluation of the rate of SDF (rSDF; SDF measured between two consecutive time points) could provide accurate information pertaining to nuclear sperm vulnerability and differential susceptibility to DNA damage. This observation has already been demonstrated in a variety of mammalian species (8)(9)(10).…”

Successful ram semen cryopreservation with lyophilized egg yolk-based extender

“…Assessing the integrity of DNA is valuable for the best identification of seriously damaged spermatozoa [21]. Among other sperm tests, evaluation of DNA integrity has been considered important as early embryo development depends on the presence of normal DNA.…”

Effect of sperm DNA fragmentation on pregnancy outcome depends on oocyte quality

“…Pregnancy is unlikely in such cases. This suggests that a sperm population without seriously fragmented DNA and in contact with spermatozoa with SDF may sustain some DNA damage in what can be referred to as an iceberg effect (21)(22)(23). In this case, if the sperm cell has suffered only slight DNA damage, the oocyte should be able to repair it, but the extent of this repair will depend on the quality of the oocyte.…”