Dynamics of the composition of a microbial consortium during start-up of a single-stage constant flow laboratory nitritation/anammox setup

Mardanov, Andrey V.; Beletskii, A. V.; Kallistova, A. Yu.; Kotlyarov, Roman Y.; Nikolaev, Yu. A.; Кевбрина, М. В.; Agarev, A. M.; Ravin, Nikolai V.; Пименов, Н. В.

doi:10.1134/s002626171606014x

Cited by 12 publications

(7 citation statements)

References 31 publications

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“…NOB were rarely detected in anammox bioreactors with microaerophilic or anaerobic conditions since they are more sensitive than AOB to lack of aeration (Dosta et al, 2015; Speth et al, 2016). However, in some cases NOB occur in anammox bioreactors even under anaerobic conditions (Liu et al, 2017), mainly members of the genus Nitrospira which were shown to carry out the commamox process (van Kessel et al, 2015; Mardanov et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Genome of a Novel Bacterium “Candidatus Jettenia ecosi” Reconstructed From the Metagenome of an Anammox Bioreactor

et al. 2019

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The microbial community of a laboratory-scale bioreactor based on the anammox process was investigated by using metagenomic approaches and fluorescent in situ hybridization (FISH). The bioreactor was initially inoculated with activated sludge from the denitrifying bioreactor of a municipal wastewater treatment station. By constantly increasing the ammonium and nitrite load, a microbial community containing the novel species of anammox bacteria “Candidatus Jettenia ecosi” developed in the bioreactor after 5 years when the maximal daily nitrogen removal rate reached 8.5 g/L. Sequencing of the metagenome of anammox granules and the binning of the contigs obtained, allowed a high quality draft genome of the dominant anammox bacterium, “Candidatus Jettenia ecosi” to be assembled. Annotation of the 3.9 Mbp long genome revealed 3970 putative protein-coding genes, 45 tRNA genes, and genes for 16S/23S rRNAs. Analysis of the genome of “Candidatus Jettenia ecosi” revealed genes involved in anammox metabolism, including nitrite and ammonium transporters, copper-containing nitrite reductase, a nitrate reductase complex, hydrazine synthase, and hydrazine dehydrogenase. Autotrophic carbon fixation could be accomplished through the Wood Ljungdahl pathway. The composition of the community was investigated through a search of 16S rRNA sequences in the metagenome and FISH analysis of the anammox granules. The presence of the members of Ignavibacteriae, Betaproteobacteria, Chloroflexi and other microbial lineages reflected the complexity of the microbial processes in the studied bioreactor performed by anammox Planctomycetes, fermentative bacteria, and denitrifiers.

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Section: Discussionmentioning

confidence: 99%

Genome of a Novel Bacterium “Candidatus Jettenia ecosi” Reconstructed From the Metagenome of an Anammox Bioreactor

et al. 2019

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“…These patterns might be associated with the fundamental differences in particle-attached and free-living bacterial community compositions (Jiang et al, 2007). Bacteria in the order Nitrosomonadales, enriched in low-salinity soils, can perform the transformation of ammonium oxidation to nitrite (Mardanov, Beletskii, & Kallistova, 2016). The family Nitrosomonadaceae, belonging to this order, functions as lithoautotrophic ammonia oxidizers, participating in the nitrogen biogeochemical processes in freshwater, and marine environments (Prosser, Head, & Stein, 2014).…”

Section: Saline-responsive Bacterial Speciesmentioning

confidence: 99%

Shifts in the soil bacterial community along a salinity gradient in the Yellow River Delta

et al. 2020

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Soil salinization has rapidly encroached from the coastline to inland areas over the past two decades in the Yellow River Delta (YRD). Soil samples were collected from low-(LSW), medium-(MSW), and high-(HSW) salinity wetlands at a depth of 0-20 cm for 16S rRNA sequencing and bioinformatic analyses. The richness and α-diversity indices were significantly lower in saline soils (ECe > 15 dS/m, HSW) than in soils those were not saline (ECe < 15 dS/m, LSW and MSW) (p < 0.05), generally showing a decreasing trend with increasing salinities. The phyla, Proteobacteria, Bacteroidetes, Chloroflexi, Acidobacteria and Planctomycetes, represented more than 70% of the bacterial community in the three wetlands, indicating the wide adaption of these phyla to salinity changes. Specifically, Proteobacteria was recognized as the most dominant (35.30%-38.59%) phylum regardless of salinity. Furthermore, bacterial composition was different among the wetlands, as revealed by β-diversity indices and analysis of similarities. Linear discriminant analysis (LDA) effect size revealed the presence of 11, 2, and 10 discriminating bacterial taxa (LDA > 4) among LSW, MSW, and HSW, respectively, implying that they can serve as bioindicators of soil salinization. Redundancy analysis, Spearman correlation analysis, and the Mantel test suggested that salinity parameters (EC, Na + , K + , Mg 2+ , Ca 2+ , Cl − , and SO 4 2−) prominently structured the bacterial community in the current study. These results suggest that the changes of bacterial composition would be induced in these LSW and MSW soils once seawater intrusion occurs.

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“…However, the vast majority of bacteria found were organotrophs. Their role in the anammox community most likely consists in the decomposition of dead cell biomass of activated sludge and EPS formed by anammox bacteria (Mardanov et al, 2016). Besides, according to metagenomics and metatranscriptomic studies available in the literature, most high-abundance heterotrophic members of the community detected (e.g., Chloroflexi, Ignavibacteria, and Armatimonadetes) have genes encoding different enzymes of nitrogen metabolism (Mardanov et al, 2019b).…”

Section: Discussionmentioning

confidence: 99%

“…"Jettenia" cells during biofilm formation on the walls of the reactors and can also protect them from overheating. So, the anammox component of the microbial community evidently exhibits high lability in dependence on the set of environmental conditions (Mardanov et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

New Insight Into the Interspecies Shift of Anammox Bacteria Ca. “Brocadia” and Ca. “Jettenia” in Reactors Fed With Formate and Folate

et al. 2022

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The sensitivity of anaerobic ammonium-oxidizing (anammox) bacteria to environmental fluctuations is a frequent cause of reactor malfunctions. It was hypothesized that the addition of formate and folate would have a stimulating effect on anammox bacteria, which in turn would lead to the stability of the anammox process under conditions of a sharp increase in ammonium load, i.e., it helps overcome a stress factor. The effect of formate and folate was investigated using a setup consisting of three parallel sequencing batch reactors equipped with a carrier. Two runs of the reactors were performed. The composition of the microbial community was studied by the 16S rRNA gene profiling and metagenomic analysis. Among anammox bacteria, Ca. “Brocadia” spp. dominated during the first run. A stimulatory effect of folate on the daily nitrogen removal rate (dN) was identified. The addition of formate led to progress in dissimilatory nitrate reduction and stimulated the growth of Ca. “Jettenia” spp. The spatial separation of two anammox species was observed in the formate reactor: Ca. “Brocadia” occupied the carrier and Ca. “Jettenia”—the walls of the reactors. Biomass storage at low temperature without feeding led to an interspecies shift in anammox bacteria in favor of Ca. “Jettenia.” During the second run, a domination of Ca. “Jettenia” spp. was recorded along with a stimulating effect of formate, and there was no effect of folate on dN. A comparative genome analysis revealed the patterns suggesting different strategies used by Ca. “Brocadia” and Ca. “Jettenia” spp. to cope with environmental changes.

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Dynamics of the composition of a microbial consortium during start-up of a single-stage constant flow laboratory nitritation/anammox setup

Cited by 12 publications

References 31 publications

Genome of a Novel Bacterium “Candidatus Jettenia ecosi” Reconstructed From the Metagenome of an Anammox Bioreactor

Genome of a Novel Bacterium “Candidatus Jettenia ecosi” Reconstructed From the Metagenome of an Anammox Bioreactor

Shifts in the soil bacterial community along a salinity gradient in the Yellow River Delta

New Insight Into the Interspecies Shift of Anammox Bacteria Ca. “Brocadia” and Ca. “Jettenia” in Reactors Fed With Formate and Folate

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