Dynamics of the Self-Assembly of Unilamellar Vesicles

Weiß, Thomas; Narayanan, Theyencheri; Wolf, Christian; Gradzielski, Michael; Panine, Pierre; Finet, Stéphanie; Helsby, W.

doi:10.1103/physrevlett.94.038303

Cited by 127 publications

(157 citation statements)

References 19 publications

Supporting

Mentioning

145

Contrasting

Order By: Relevance

“…One important application is the study of protein and RNA folding, processes that involve a large dynamic range in both length and time scales, with many important processes involved in hydrophobic collapse occurring on the microsecond to millisecond time scale (Kathuria et al, 2011;Sosnick & Barrick, 2011;Svergun & Koch, 2003;Thirumalai et al, 2001;Woodson, 2010). Similarly, ligand and RNA/DNA binding (Wee et al, 2012), assembly of lipid bilayer structures and nano-particle-based drug delivery systems (Johnson & Prud'homme, 2003), vesicle formation (Weiss et al, 2005;Guida, 2010), protein association (Doyle et al, 2004) and conformational dynamics (Chattopadhyay et al, 2002;Werner et al, 2006;Srajer & Royer, 2008) also typically occur on a sub-millisecond timescale. The most common way to experimentally investigate such processes is to initiate them by rapid mixing of individual components and/or changing the solvent, pH or ionic strength, using various microfluidic devices.…”

Section: Introductionmentioning

confidence: 99%

Sub-millisecond time-resolved SAXS using a continuous-flow mixer and X-ray microbeam

et al. 2013

View full text Add to dashboard Cite

Small-angle X-ray scattering (SAXS) is a well established technique to probe the nanoscale structure and interactions in soft matter. It allows one to study the structure of native particles in near physiological environments and to analyze structural changes in response to variations in external conditions. The combination of microfluidics and SAXS provides a powerful tool to investigate dynamic processes on a molecular level with sub-millisecond time resolution. Reaction kinetics in the sub-millisecond time range has been achieved using continuous-flow mixers manufactured using micromachining techniques. The time resolution of these devices has previously been limited, in part, by the X-ray beam sizes delivered by typical SAXS beamlines. These limitations can be overcome using optics to focus X-rays to the micrometer size range providing that beam divergence and photon flux suitable for performing SAXS experiments can be maintained. Such micro-SAXS in combination with microfluidic devices would be an attractive probe for time-resolved studies. Here, the development of a high-duty-cycle scanning microsecond-timeresolution SAXS capability, built around the Kirkpatrick-Baez mirror-based microbeam system at the Biophysics Collaborative Access Team (BioCAT) beamline 18ID at the Advanced Photon Source, Argonne National Laboratory, is reported. A detailed description of the microbeam small-angle-scattering instrument, the turbulent flow mixer, as well as the data acquisition and control and analysis software is provided. Results are presented where this apparatus was used to study the folding of cytochrome c. Future prospects for this technique are discussed.

show abstract

Section: Introductionmentioning

confidence: 99%

Sub-millisecond time-resolved SAXS using a continuous-flow mixer and X-ray microbeam

et al. 2013

View full text Add to dashboard Cite

show abstract

“…vesicles, [8,9] disk-shaped vesicle, [10] rods, [11] small disks, [12] to micelles. [13,14] Self-organized structure from binary amphiphiles mixtures was studied both experimentally and theoretically.…”

Section: Introductionmentioning

confidence: 99%

Self-Organization of Polyurethane Pre-Polymers as Studied by Self-Consistent Field Theory

Tuinier

Casteren

et al. 2015

Macromol. Theory Simul.

View full text Add to dashboard Cite

Using self‐consistent field (SCF) theory, we studied the self‐assembly characteristics of polyurethane pre‐polymer dispersions in aqueous solutions. With a molecularly detailed model implementing the Scheutjens–Fleer discretization scheme, it is shown how the stability, equilibrium size, and internal structure of the (swollen) micelles in polyurethane (PU) dispersions depend on the chemical structure and the molecular composition of the charged pre‐polymer mixtures. The stability region of these micelles is found to increase when acid groups become deprotonated and when the ionic strength is lowered. Insight into the physical–chemical behavior of PU pre‐polymer dispersions is important for the subsequent process of film formation from the PU dispersions for the final coating properties.

show abstract

“…The selfassembly dynamics was studied experimentally by timeresolved scattering techniques ͑light, neutron, and X ray͒. [13][14][15][16][17] Typically, the surfactants are found to aggregate into disklike micelles, which grow and transform into a vesicle when their radius exceeds a critical size. The final vesicle size is probably more controlled by kinetics than by thermodynamics.…”

Section: Introductionmentioning

confidence: 99%

Dynamics of vesicle self-assembly and dissolution

Noguchi

Gompper

2006

The Journal of Chemical Physics

View full text Add to dashboard Cite

The dynamics of membranes is studied on the basis of a particle-based meshless surface model, which was introduced earlier ͓Phys. Rev. E 73, 021903 ͑2006͔͒. The model describes fluid membranes with bending energy and-in the case of membranes with boundaries-line tension. The effects of hydrodynamic interactions are investigated by comparing Brownian dynamics with a particle-based mesoscale solvent simulation ͑multiparticle collision dynamics͒. Particles self-assemble into vesicles via disk-shaped membrane patches. The time evolution of assembly is found to consist of three steps: particle assembly into discoidal clusters, aggregation of clusters into larger membrane patches, and finally vesicle formation. The time dependence of the cluster distribution and the mean cluster size is evaluated and compared with the predictions of Smoluchowski rate equations. On the other hand, when the line tension is suddenly decreased ͑or the temperature is increased͒, vesicles dissolve via pore formation in the membrane. Hydrodynamic interactions are found to speed up the dynamics in both cases. Furthermore, hydrodynamics makes vesicle more spherical in the membrane-closure process.

show abstract

Dynamics of the Self-Assembly of Unilamellar Vesicles

Cited by 127 publications

References 19 publications

Sub-millisecond time-resolved SAXS using a continuous-flow mixer and X-ray microbeam

Sub-millisecond time-resolved SAXS using a continuous-flow mixer and X-ray microbeam

Self-Organization of Polyurethane Pre-Polymers as Studied by Self-Consistent Field Theory

Dynamics of vesicle self-assembly and dissolution

Contact Info

Product

Resources

About