Dynorphin A(6-12) Analogs Suppress Thermal Edema

Wei, E.T.; Thomas, Holly A.; Gjerde, E.-A. B.; Reed, Rolf K.; Буров, С. В.; Korol'kov, V. I.; Glynskaya, Oxana V; Dorosh, Marina; Vlasov, G. P.

doi:10.1016/s0196-9781(97)00473-7

Cited by 10 publications

(3 citation statements)

References 25 publications

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“…The immunoregulation observed may be facilitated by a range of opioid and non-opioid fragments, produced following biotransformation of DYN 1-17 at the site of inflammation [6]. Outside of traditional opioid receptor-mediated effects, evidence of non-opioid receptors roles for opioid peptides include action at melanocortin receptors [27,28], bradykinin receptors [29], Nmethyl-D-aspartate (NMDA) receptor complex [30] and recently the TLR4 [20], therefore the overarching effects of biotransformed opioid peptides may involve a number of possible mechanisms. It is likely that DYN 3-14, the most abundant fragment of DYN 1-17 produced in inflamed tissue, modulates inflammatory responses through one of these mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory effects of dynorphin 3-14 on the lipopolysaccharide-induced toll-like receptor 4 signalling pathway

et al. 2017

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.Inhibitory effects of dynorphin 3-14 on the lipopolysaccharide-induced toll-like receptor 4 signalling pathway.Peptides http://dx.doi.org/10.1016/j.peptides. 2017.02.004 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.  DYN 3-14 inhibits NF-κB/p65 activation in response to LPS stimulation. DYN 3-14 differentially modulates the release of IL-1β and TNF-α induced by LPS. DYN 3-14 inhibits LPS-induced TLR4 signalling in a competitive manner. The endogenous opioids DYN 3-14 is a potential TLR4 antagonist.

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Section: Discussionmentioning

confidence: 99%

Inhibitory effects of dynorphin 3-14 on the lipopolysaccharide-induced toll-like receptor 4 signalling pathway

et al. 2017

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“…The lowering of P if will increase the transmural driving pressure during the initial phase of an inflammatory challenge. The lowering of P if measured in tracheal tissue of rats following an inflammatory challenge ranges from À4 to À10 mmHg (Gjerde et al, 1997(Gjerde et al, , 2000Koller and Reed, 1992;Wei et al, 1998;Woie et al, 1993;Woie and Reed, 1997;Woie and Reed, 1994;Woie and Reed, 1996a;. Under normal (non-inflammatory) conditions P if is slightly subatmospheric and the mean average and standard deviation measured over 60 or 90 min registration period for trachea is À1.45 7 0.36 mmHg.…”

Section: Introductionmentioning

confidence: 98%

“…Under normal (non-inflammatory) conditions P if is slightly subatmospheric and the mean average and standard deviation measured over 60 or 90 min registration period for trachea is À1.45 7 0.36 mmHg. This average is taken from a total of 13 studies and 17 series of experiments containing all together 132 control animals giving a average of 7-8 animals per series (Gjerde et al, 1997(Gjerde et al, , 2000(Gjerde et al, , 2002(Gjerde et al, , 2003Koller and Reed, 1992;Koller et al, 1993;Wei et al, 1998;Woie et al, 1993Woie and Reed, 1994. Figures from previous studies Gjerde et al, 2002) illustrate that the levels of P if in vehicle treated animals are constant throughout the 60 min recording period.…”

Section: Introductionmentioning

confidence: 99%

Problems in physiological experimental animal models investigated with factorial design

Gjerde

Eide

Brønstad

et al. 2004

Journal of Experimental Animal Science

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In the present study we investigated four variables using factorial design to decide if any of these could explain the variations in the control measurements of interstitial fluid pressure (P if ) in rat trachea that were experienced. This approach requires only a fraction of the animals normally needed when studying each factor separately. P if in tracheal tissue was measured with the servocontrolled counterpressure system using sharpened micropipettes. The measurements were performed over a period of 60 min and are presented as mean for every 15 min period. The factors investigated in the study were: three strains of female rats (Strain) two brands of diets (Food); two breeder companies (Source); and finally two batches of the same set of animals to repeat the experiment twice (Week), using a total of 48 animals. There was a highly significant effect within Strain the first week (p ¼ 0:007), but this response was not observed the second week. The interaction between Strain Â Week was significant (p ¼ 0:007) while the main effects Strain or Week alone were not significant. The response pattern for Strain and Food was inconsistent for the two experimental weeks studied. These experiments made it possible for us to simultaneously test several factors and exclude these factors as the reason for the observed changes in our experiments since the experiments did not allow the conclusion that one or several of these factors could explain the variation in P if : r

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Dynorphin A 1–17 biotransformation in inflamed tissue, serum and trypsin solution analysed by liquid chromatography–tandem mass spectrometry

et al. 2012

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Dynorphin A 1-17 (DYN A) is an endogenous neuropeptide that is of interest due to its diverse roles in analgesia, inflammation and addiction. Upon release, DYN A is subject to metabolism by a range of enzymes and its biotransformation is dependent on the site and environment into which it is released. In this study, we investigated the biotransformation of DYN A in rat inflamed tissue at pH 7.4 and 5.5, in rat serum and in trypsin solution. DYN A-porcine was incubated at 37 °C in each matrix over a range of incubation periods. The resultant fragments were separated using a C4 column and detected by mass spectrometry using total ion current mode. Incubation of DYN A in trypsin solution and in rat serum resulted in 6 and 14 fragments, respectively. Incubation in inflamed rat paw tissue occasioned 21 fragments at pH 7.4 and 31 fragments at pH 5.5. Secondary breakdown of some larger primary fragments was also observed in this study.

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Dynorphin A(6-12) Analogs Suppress Thermal Edema

Cited by 10 publications

References 25 publications

Inhibitory effects of dynorphin 3-14 on the lipopolysaccharide-induced toll-like receptor 4 signalling pathway

Inhibitory effects of dynorphin 3-14 on the lipopolysaccharide-induced toll-like receptor 4 signalling pathway

Problems in physiological experimental animal models investigated with factorial design

Dynorphin A 1–17 biotransformation in inflamed tissue, serum and trypsin solution analysed by liquid chromatography–tandem mass spectrometry

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