Dysregulation of hepatocyte cell cycle and cell viability by hepatitis B virus

Chin, Rey; Nachbur, Ueli; Earnest-Silveira, Linda; Bankovacki, Aleksandra; Koeberlein, Bernd; Zentgraf, Hanswalter; Bock, C.‐Thomas; Torresi, Joseph

doi:10.1016/j.virusres.2009.09.012

Cited by 9 publications

(11 citation statements)

References 62 publications

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“…As shown in Table 2, most of these miRNA are predicted to target the signal pathways of angiogenesis, apoptosis, oxidative stress response, p53, Fas, interleukin, IFN‐γ and inflammation. These findings are consistent with previous studies that suggest that HBV replication modulates the process of carcinogenesis, apoptosis and oxidative stress response of the cells 26–29 …”

Section: Discussionsupporting

confidence: 94%

“…These findings are consistent with previous studies that suggest that HBV replication modulates the process of carcinogenesis, apoptosis and oxidative stress response of the cells. [26][27][28][29] Except for these highly differentially expressed miRNA, we also found that several of the low/medium differentially expressed miRNA (supplemental Table S1) changed similarly to those in previous studies. For example, miR-181a (3.589), miR-196b (3.114), miR-146a (4.043) and miR-17 (0.416) had similar changes, as previously reported.…”

Section: Hbv Replication Alerts Mirna Expression In Hepg2 Cellssupporting

confidence: 85%

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Characterization of microRNA expression profiles associated with hepatitis B virus replication and clearance in vivo and in vitro

Pan

Qian

et al. 2012

J of Gastro and Hepatol

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Section: Discussionsupporting

confidence: 94%

Section: Hbv Replication Alerts Mirna Expression In Hepg2 Cellssupporting

confidence: 85%

Characterization of microRNA expression profiles associated with hepatitis B virus replication and clearance in vivo and in vitro

Pan

Qian

et al. 2012

J of Gastro and Hepatol

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“…However, the viral genome is introduced by integration or transfection rather than infection [12,13]. Therefore, these cell lines are unsuitable for studying the mechanism of the early stages of virus-cell interactions, including viral attachment, penetration, and uncoating.…”

Section: Discussionmentioning

confidence: 99%

Hepatitis B virus infection and replication in human bone marrow mesenchymal stem cells

Xing

Shao

et al. 2011

Virol J

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BackgroundHepatitis B virus (HBV) infection is a blood borne infectious disease that affects the liver. Human bone marrow mesenchymal stem cells (BMSCs) may serve as a cell source for adult stem cell transplantation in liver repair. However, the susceptibility of human BMSCs to HBV infection is poorly understood. The aim of this study was to investigate the infection and replication of HBV in cultures of human BMSCs.ResultsHuman BMSCs were confirmed using flow cytometry. Intracellular HBV DNA was detected at d 2 after infection and maintained at relatively high levels from d 6 to d 12. The maximal level of intracellular HBV DNA was 9.37 × 105 copies/mL. The extracellular HBV DNA was observed from d 3 to d 15, and the levels ranged from 3.792 × 102 copies/mL to 4.067 × 105 copies/mL. HBsAg in the culture medium was detected from d 2 to d 16. HBeAg secretion was positive from d 5 to d 13. HBcAg constantly showed positive signals in approximately 7%-20% of BMSCs from 2 days after exposure. Intracellular HBV covalently closed circular DNA (cccDNA) could be detected as early as 2 days postinfection, and strong signals were obtained with increasing time.ConclusionHBV can infect and replicate in human BMSCs. Human BMSCs may be a useful tool for investigating HBV life-cycle and the mechanism of initial virus-cell interactions.

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“…Relationship between MTDH expression and clinicopathological parameters in patients with HBV-related HCC. (34)(35)(36). Additionally, HBx has been shown to activate the transcription factors NF-κB (37) and AP-1 (38).…”

Section: Discussionmentioning

confidence: 99%

Prognostic significance of metadherin overexpression in hepatitis B virus-related hepatocellular carcinoma

et al. 2012

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Abstract. Metadherin (MTDH), which is an HIV-1 or TNF-α-inducible transcript, has a crucial role in several types of cancer by regulating multiple cellular signaling processes. However, to date, the role of MTDH in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) is still unclear. In the present study, we detected MTDH expression in normal liver, chronic hepatitis B and HBV-related HCC tissues. The data showed that MTDH expression levels were elevated in the hepatitis B tissues and especially in the HBV-related HCC tissues compared to normal liver tissues. There was a trend for gradually increased MTDH expression from normal liver tissue to hepatitis B and HBV-related HCC tissues. Furthermore, a statistical analysis revealed that MTDH expression significantly correlated with the American Joint Committee on Cancer (AJCC, 7th edition) stage (P=0.020), T classification (P= 0.007), N classification (P= 0.044), vascular invasion (P=0.006) and histological differentiation (P=0.020) in the HBV-related HCC patients. In addition, patients with high MTDH levels had shorter survival times compared to those with low MTDH expression (P=0.001). Taken together, these results suggest that MTDH could be a potential prognostic marker for overall survival and tumor progression and a chemotherapeutic target in HBV-related HCC patients.

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Dysregulation of hepatocyte cell cycle and cell viability by hepatitis B virus

Cited by 9 publications

References 62 publications

Characterization of microRNA expression profiles associated with hepatitis B virus replication and clearance in vivo and in vitro

Characterization of microRNA expression profiles associated with hepatitis B virus replication and clearance in vivo and in vitro

Hepatitis B virus infection and replication in human bone marrow mesenchymal stem cells

Prognostic significance of metadherin overexpression in hepatitis B virus-related hepatocellular carcinoma

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