Dysregulation of KIF14 regulates the cell cycle and predicts poor prognosis in cervical cancer: a study based on integrated approaches

Abstract

Cervical cancer (CC) is the most common malignant tumor in females. Although persistent high-risk human papillomavirus (HPV) infection is a leading factor that causes CC, few women with HPV infection develop CC. Therefore, many mechanisms remain to be explored, such as aberrant expression of oncogenes and tumor suppressor genes. To identify promising prognostic factors and interpret the relevant mechanisms of CC, the RNA sequencing profile of CC was downloaded from the Cancer Genome Atlas and the Gene Expression Omnibus databases. The GSE63514 dataset was analyzed, and differentially expressed genes (DEGs) were obtained by weighted coexpression network analysis and the edgeR package in R. Fifty-three shared genes were mainly enriched in nuclear chromosome segregation and DNA replication signaling pathways. Through a protein-protein interaction network and prognosis analysis, the kinesin family member 14 (KIF14) hub gene was extracted from the set of 53 shared genes, which was overexpressed and associated with poor overall survival (OS) and disease-free survival (DFS) of CC patients. Mechanistically, gene set enrichment analysis showed that KIF14 was mainly enriched in the glycolysis/gluconeogenesis signaling pathway and DNA replication signaling pathway, especially in the cell cycle signaling pathway. RT-PCR and the Human Protein Atlas database confirmed that these genes were significantly increased in CC samples. Therefore, our findings indicated the biological function of KIF14 in cervical cancer and provided new ideas for CC diagnosis and therapies.

Role of kinesin family member 14 in disease monitoring and prognosis in patients with gastrointestinal cancer

“…In addition, KIF14 was upregulated in tumor tissues and could predict unfavorable outcomes in patients with ovarian cancer (29). These aforementioned studies indicated that in numerous types of cancer, KIF14 might be a promoter of cancer progression, and could be a potential biomarker for prognosis (21,(24)(25)(26)(27)(28)(29).…”

“…As a crucial member of the kinesin family, KIF14 has been reported to be a regulator of tumor development and progression in previous studies and could also be a potential biomarker in the management of patients with cancer ( 24 , 25 ). A prior study revealed that KIF14 inhibition using small interfering (si)RNA could repress proliferation and enhance apoptosis in medulloblastoma cells, while KIF14 inhibition using short hairpin RNA could suppress cell viability, colony formation, invasion, migration and tumor sphere formation in medulloblastoma cells ( 18 ).…”

Chromatin Separation Regulators Predict the Prognosis and Immune Microenvironment Estimation in Lung Adenocarcinoma

“…HJURP is reported to be an oncogene that promotes cancer cell proliferation, migration, and invasion (Chen et al, 2018;Wei et al, 2019;Serafim et al, 2020;Lai et al, 2021). KIF14 is confirmed to promote cancer cell proliferation and contribute to chemoresistance (Singel et al, 2014;Xiao et al, 2021). Silencing TTK is also found to inhibit the proliferation and invasion and increase radiosensitivity and chemosensitivity of cancer cells (Chen S. et al, 2019;Huang et al, 2020;Zhang et al, 2021b;Qi et al, 2021).…”

Metadata analysis to explore hub of the hub-genes highlighting their functions, pathways and regulators for cervical cancer diagnosis and therapies

“…According to the existing literature, CDK2 was a more promising therapeutic target for cervical cancer [ 37 , 59 ]. CHEK1 hub gene produced a high score expression value in CC tissues compared with normal tissues and was considered as a significant upregulated expressed gene (P < 0.01) [ 20 , 44 ].…”