Dysregulation of microRNA146a-5p expression in systemic lupus erythematosus females: Diagnostic potential and association with ocular manifestations

Labib, Dina; Koptan, Dina M.T.; Ghoniem, Shada; Salah, Shaymaa Hassan; Shazly, Reem El; Refai, Rasha M. El

doi:10.1016/j.ejr.2019.07.004

Cited by 11 publications

(9 citation statements)

References 35 publications

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“…Our study showed a significantly increased level of miR-146a in PBMCs of SLE patients compared to controls. Our research agrees that the miR-146a level was overexpressed in SLE patients compared to controls in Europeans [ 50 ] and Egyptian [ 51 ] populations. In contrast, the miR-146a level was decreased as compared to healthy controls in Egyptian [ 52 ], European [ 23 ], and Chinese [ 25 , 53 , 54 ] populations.…”

Section: Discussionsupporting

confidence: 90%

mir-146a genetic polymorphisms in systemic lupus erythematosus patients: Correlation with disease manifestations

El-Akhras

Ali

El‐Masry

et al. 2022

Non-coding RNA Research

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Section: Discussionsupporting

confidence: 90%

mir-146a genetic polymorphisms in systemic lupus erythematosus patients: Correlation with disease manifestations

El-Akhras

Ali

El‐Masry

et al. 2022

Non-coding RNA Research

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“…Similarly, Shahid et al 40 found a substantial drop in miR-146a expression in the plasma of acute lymphoblastic leukemia (ALL) patients after treatment. On the contrary, Labib et al 11 found that miRNA146a tended to be elevated in SLE patients with LN after treatment. In contrast to our study, Tang et al 32 observed that miR-146a is upregulated in treated lupus against non-treated ones.…”

Section: Discussionmentioning

confidence: 91%

“…Several autoimmune disorders, including psoriasis, 7 rheumatoid arthritis (RA), 8 osteoarthritis, 9 and systemic lupus erythematosus (SLE), have dysregulated miR-146a expression (SLE). 10 , 11 …”

Section: Introductionmentioning

confidence: 99%

Crosstalk between miR-146a and pro-inflammatory cytokines in patients with systemic lupus erythematosus

El-Akhras

Talaat

El‐Masry

et al. 2023

Int J Immunopathol Pharmacol

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microRNA-146a (miR-146a) plays an essential role in immune anomalies and organ injury of systemic lupus erythematosus (SLE) by regulating the disease’s inflammation and complications. Here, we analyzed the expression of miR-146a in SLE and a panel of pro-inflammatory cytokines (IL-1, IL-6, IL-8, IL-17, and TNF-α). Association between all measured parameters and the disease’s clinical manifestation and response to treatment was monitored. Our study populations were 113 SLE patients and 104 healthy volunteers. miR-146a expression in peripheral blood mononuclear cells (PBMCs) was measured by quantitative real-time PCR (RT-qPCR). The content of the plasma cytokines (IL-1β, IL-6, IL-8, IL-17, and TNF-α) was detected by enzyme-linked immunosorbent assay (ELISA). Compared with healthy controls, miR-146a expression was significantly increased ( p < 0.05) in lupus patients. The analysis of the receiver operator characteristic curve (ROC) of miR-146a showed 91% sensitivity and 70% specificity. IL-1β, IL-6, and IL-17 cytokines were significantly increased ( p < 0.001), while IL-8 and TNF-α were significantly decreased ( p < 0.001) in SLE patients against controls. The expression of miR-146a and TNF-α was upregulated considerably in SLE patients with severe disease activity. miR-146a expression was positively correlated with IL-6. Our results pointed to the elevation of miR-146a as a trade marker of SLE patients. Reduction of IL-8 and TNF-α in combination with an elevation of IL-1β, IL-6, and IL-17 might refer to miR-146a’s dual effect in controlling inflammation in lupus. Although we shed some light on the role of miR-146a in SLE, further study is recommended to improve our results.

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“…An earlier study by our group demonstrated increased serum miR-146a-5p in SLE patients with retinopathy compared to SLE patients without retinopathy. 32 Previous studies demonstrate the dysregulated expression of miR-146a-5p in ocular component tissues, including the vitreous humor in patients with melanoma and retinal endothelial cells in patients with diabetic retinopathy. 33 In our study, weadded plasma miRNA146 to correlate with choroidal thickness.It was found to be highly expressed in patients with SLE and especially nephritic lupus.…”

Section: Discussionmentioning

confidence: 97%

<p>Choroidal Thickness and microRNA146 in Lupus Nephritis Patients</p>

Salah

Makled

Elmekawey

et al. 2020

OPTH

Self Cite

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Purpose: To evaluate the choroidal thickness (CT) in the macular area in patients with lupus nephritis and to compare the results with both non-nephritic patients and normal control. To assess the relation of CT to serum microRNA146, disease duration, activity index, and medications.Patients and Methods: Thirty-five SLE patients and thirty normal healthy controls were enrolled for this cross-sectional prospective study. All participants have undergone optical coherence tomography using RTVue OCT (Optovue Inc., Fremont, CA, USA). The scan used was the macular cross 6-mm line. We measured CT from the posterior edge of the retinal pigment epithelium (RPE) to the choroid-sclera junction at subfovea, and 750 µm both temporal and nasal to the fovea.Results: The mean central subfoveal CT in patients was 275.7 ± 41.0 µm (214-374 µm), and the mean central subfoveal CT in the control group was 364.5± 23.0 µm (323-411µm). There was a significant thinning at all three points in patients compared to the control group (p<0.001, Mann-Whitney U-test). In the patients group, subfoveal choroid in non-nephritic subgroup showed significant thinning compared to nephritic subgroup (p=0.032, Mann-Whitney U-test). Drusen-like deposits (DLDs) were detected in 22.9% (8/35) of patients and none in control (p=.023). MiRNA146 showed a significant positive correlation with nephritic lupus patients (r=0.036, P=0.04). Conclusion:The choroidal thickness was significantly thicker among the nephritic lupus patients as compared to the non-nephritic subgroup. Both SLE patients' subgroups are thinner than normal control. Subfoveal choroidal thickening can be considered a biomarker in nephritic lupus especially in conjunction with an increase in miRNA146a. All SLE patients are at risk of small Drusen-like deposits.

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Dysregulation of microRNA146a-5p expression in systemic lupus erythematosus females: Diagnostic potential and association with ocular manifestations

Cited by 11 publications

References 35 publications

mir-146a genetic polymorphisms in systemic lupus erythematosus patients: Correlation with disease manifestations

mir-146a genetic polymorphisms in systemic lupus erythematosus patients: Correlation with disease manifestations

Crosstalk between miR-146a and pro-inflammatory cytokines in patients with systemic lupus erythematosus

<p>Choroidal Thickness and microRNA146 in Lupus Nephritis Patients</p>

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