Dysregulation of PVT1 and NEAT1 lncRNAs in pituitary adenomas

Ghafouri‐Fard, Soudeh; Abbasi, Farzaneh; Nicknam, Amir; Hussen, Bashdar Mahmud; Eslami, Solat; Dilmaghani, Nader Akbari; Taheri, Mohammad; Sharifi, Guive

doi:10.1016/j.prp.2023.154573

Cited by 3 publications

(1 citation statement)

References 16 publications

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“… 6 In the pathobiology of cancer, thousands of intracellular factors are altered, including signalling pathways, energy metabolism, gene transcription regulators, etc. 7 , 8 , 9 , 10 Signalling pathways are often stimulated by signalling molecules found on cell membranes or within the cytoplasm, such as growth factors, hormones, or ions. For signal transduction, a variety of intracellular chemicals known as secondary messengers are used, an important one of which is cyclic adenosine monophosphate (cAMP).…”

Section: Introductionmentioning

confidence: 99%

Expression of cAMP and oxidative phosphorylation‐related lncRNAs in non‐functioning pituitary adenomas

Taheri,

Nicknam,

Bagan

et al. 2023

J Cellular Molecular Medi

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Non‐functioning pituitary adenomas (NFPAs) are benign lesions in the pituitary gland with important morbidities. In this study, based on a bioinformatics analysis to identify the genes and pathways that display significant differences between tumour tissues of NFPA patients and normal pituitary tissues, we selected lncRNAs related to cAMP and oxidative phosphorylation pathways, namely DNAH17‐AS1, LINC00706 and SLC25A5‐AS1. Then, we aimed to investigate by means of RT‐qPCR, the expression of these lncRNAs along with two other lncRNAs, namely CADM3‐AS1 and MIR7‐3HG in NFPA samples compared to that in healthy tissues adjacent to the tumours. Transcripts of DNAH17‐AS1, LINC00706 and MIR7‐3HG were lower in NFPA samples compared with controls (Expression ratios (95% CI) = 0.43 (0.23–0.78), 0.58 (0.35–0.96) and 0.58 (0.35–0.96); p‐values = 0.009, 0.025 and 0.036, respectively). AUC values of ROC curves of DNAH17‐AS1, LINC00706 and MIR7‐3HG were 0.62, 0.61 and 0.62, respectively. Expression of CADM3‐AS1 was associated with the gender of patients in a way that it was lower in female patients (p‐value = 0.04). The level of SLC25A5‐AS1 was lower in subjects with disease duration lower than 1 year (p‐value = 0.048). We showed dysregulation of three lncRNAs in NFPA tissues and potentiates these lncRNAs as important regulators of pathogenic events in these tumours.

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Section: Introductionmentioning

confidence: 99%

Expression of cAMP and oxidative phosphorylation‐related lncRNAs in non‐functioning pituitary adenomas

Taheri,

Nicknam,

Bagan

et al. 2023

J Cellular Molecular Medi

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Expression pattern of lncRNAs in pituitary adenomas

Ghafouri‐Fard

Khaledabadi

Najafi

et al. 2023

Pathology - Research and Practice

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Long Noncoding RNAs Expressed in Mouse Pituitary Development and Mature Hormone-Producing Cells

Brinkmeier,

George,

Cheung

et al. 2024

Endocrinology

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Mammalian genomes contain thousands of genes for long non-coding RNA (lncRNAs), some of which have been shown to affect protein-coding gene expression through diverse mechanisms. The lncRNA transcripts are longer than 200 nucleotides and are often capped, spliced and polyadenylated, but not translated into protein. Nuclear lncRNAs can modify chromatin structure and transcription in trans or cis by interacting with the DNA, forming R-loops, and recruiting regulatory proteins. Not much is known about the role of lncRNA in pituitary gland differentiation and function. We mined transcriptome data from mouse pituitary glands collected at embryonic days 12.5 and 14.5 and identified over 200 different lncRNA transcripts. To develop a research resource for the study of lncRNA, we used pituitary cre transgenes to tag pituitary cell types in adult mice with fluorescent markers, and enriched for thyrotropes, gonadotropes, and somatotropes using fluorescence activated cell sorting. We determined the transcriptome of each cell population using RNA sequencing and mined the data for lncRNA. We detected hundreds of lncRNAs in adult pituitary cells, a few were located immediately nearby genes that encode pituitary hormones or lineage-specific transcription factors. The location of these lncRNAs suggests the possibility of a cis-acting regulatory role in pituitary development or function, and we observe coordinated expression of two of them with their putative target genes in transgenic mice. This research resource sets the foundation for examining the actions of lncRNAs on their putative target genes and determining whether they have roles during development and in response to physiological demand.

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Dysregulation of PVT1 and NEAT1 lncRNAs in pituitary adenomas

Cited by 3 publications

References 16 publications

Expression of cAMP and oxidative phosphorylation‐related lncRNAs in non‐functioning pituitary adenomas

Expression of cAMP and oxidative phosphorylation‐related lncRNAs in non‐functioning pituitary adenomas

Expression pattern of lncRNAs in pituitary adenomas

Long Noncoding RNAs Expressed in Mouse Pituitary Development and Mature Hormone-Producing Cells

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