E. coli RNAase P has a required RNA component in vivo

Kole, Ryszard; Baer, Madeline; Stark, Benjamin C.; Altman, Sidney

doi:10.1016/0092-8674(80)90079-3

Cited by 129 publications

(80 citation statements)

References 10 publications

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“…Although RNase P is an essential endonuclease (17)(18)(19) best known for its role in tRNA 5 0 leader maturation (Table 1), to date, several other substrates are known to be processed by this complex (Table 1). Universally, the RNA subunit is able to independently perform catalysis (49)(50)(51) although its enzymatic activity is greatly enhanced in the presence of the protein subunits.…”

Section: Function and Substratesmentioning

confidence: 99%

Ribonucleases P/MRP and the Expanding Ribonucleoprotein World

et al. 2012

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SummaryOne of the hallmarks of life is the widespread use of certain essential ribozymes. The ubiquitous ribonuclease P (RNase P) and eukaryotic RNase MRP are essential complexes where a structured, noncoding RNA acts in catalysis. Recent discoveries have elucidated the three-dimensional structure of the ancestral ribonucleoprotein complex, suggested the possibility of a proteinonly composition in organelles, and even noted the absence of RNase P in a non-free-living organism. With respect to these last two findings, import mechanisms for RNases P/MRP into mitochondria have been demonstrated, and RNase P is present in organisms with some of the smallest known genomes. Together, these results have led to an ongoing debate regarding the precise definition of how ''essential'' these ribozymes truly are. IUBMBIUBMB Life, 64(6): [521][522][523][524][525][526][527][528] 2012

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Section: Function and Substratesmentioning

confidence: 99%

Ribonucleases P/MRP and the Expanding Ribonucleoprotein World

et al. 2012

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“…For example, Ul RNA has been linked to mRNA splicing (Lerner et al, 1980;Rogers and Wall, 1980) while the RNA moiety of RNAase P has been shown to be absolutely required for its activity (Kole et al, 1980). RNA has even been shown to be capable of making and breaking phosphodiester bonds in the complete absence of protein (Kruger et al, 1982).…”

Section: Introductionmentioning

confidence: 99%

Structure-function relations in E. coli 16S RNA

Thompson¹,

Hearst²

1983

Cell

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“…At present only single mutations are available in the rnc and me genes, whereas at least four mutations are known to affect RNase P. These four mutations fall into two genes [31,32]. While it is not known whether the mutation in the rnc gene affects the structure of RNase III, the mutations in the me and the rnp genes do affect the structure of the corresponding enzymes [20,32,33].…”

Section: Genetics Of Rna Processingmentioning

confidence: 99%

Processing of bacterial RNA

Apirion

Gegenheimer

1981

FEBS Letters

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Processing of RNA is a feature of RNA metabolism which contributes to the determination of the fmal population of active RNA molecules in the cell. Processing is the sum of events which converts a primary RNA transcript into a functional molecule. RNA processing in bacteria consists mainly of two parts, modification of nucleotides and cutting and trimming of the molecule; here, we shall concentrate only on the latter. There is ample evidence that rRNA and tRNA go through extensive processing events in the bacterial cell (this area is covered by a number of articles in recent symposia [ 1,2 J), During the processing events, which occur mainly during transcription [3], specific endoribonucleases introduce cuts into the growing transcripts, At present at least three such nucleases are known in Escher&&r co&: RNase P [4]; RNase III [3,S J; review cannot be all-inclusive, and will reflect our own bias. Because of space limitations we shall emphasize only certain aspects of RNA processing and in each area we shall be able to mention only some of the references.)Mutants which affect these three enzymes are available and strains have been constructed which are defective in one, two or three of these enzymes [9]. By studying tRNA and rRNA synthesis at temperatures permissive and non-~rmissive for these strains, it was deduced that these three enzymes are the major participants in the primary processing (processing which occurs during transcription) of tRNA and rRNA molecules in the cell and that there is probably one more enzyme which participates in endonucleolytic primary processing of rRNA and tRNA [IO].While primary processing of rRNA yields in E. coli the immediate precursors of the mature rRNA species, it is the secondary processing events which yield the ~~sevier~~#rth-~o~lund Biomedical Press final mature RNAs: 16 S, 23 S and 5 S. At present, while the gross features of primary rRNA processing are emerging, knowledge about secondary RNA processing is very minimal. However, there is one essential feature which seems to distinguish these two processes, i.e., namely the requirement for proteins. While all steps of RNA processing occur in the cell at the level of ribonucleoprotein particles, there does not seem to be a need for any protein beside the RNA processing enzymes in primary processing, whereas in secondary processing the substrate seems to be obligatorily an RNP particle [ 1 l-l 3 J. The overall process of primary and secondary rRNA processing in E. coli is depicted in fig.1. Enzymes involved in RNA processingA large number of enzymes has been implicated in RNA processing. However, of these only five are sufficiently characterized to be dealt with here. These are RNase P [14,15J, RNase III 15,161, RNase E 181, RNase D [17J and RNase M.5 [18].From analysis of tRNA precursors accumulated in RNase P'S mutants, it is evident that RNase P is required for 5'-maturation of most E. coli tRNA species [ 1.51. RNase P is an interesting processing enzyme since its enzymatic activity requires both a polypeptide and an ...

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E. coli RNAase P has a required RNA component in vivo

Cited by 129 publications

References 10 publications

Ribonucleases P/MRP and the Expanding Ribonucleoprotein World

Ribonucleases P/MRP and the Expanding Ribonucleoprotein World

Structure-function relations in E. coli 16S RNA

Processing of bacterial RNA

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