E-test antibiotic susceptibility of E.coli strains isolated from hospital acquired infections of Imam Khomeini hospital, Ilam, Iran

Bimanand, Lida; Sadeghifard, Nourkhoda; Jalilian, Farid Azizi; Hossainzadegan, Hassan; Darbidi, Sattar Mohammadi; Hemahian, Ali; Ghafourian, Sobhan; Pakzad, Iraj

doi:10.18869/acadpub.jbrms.4.1.17

Cited by 3 publications

(5 citation statements)

References 4 publications

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“…coli and P. aeruginosa bacteria were included in gramnegative bacteria which are known to be resistant to various antibiotics. This condition is supported by the ability of E. coli to produce extended -spectrum β-Lactamases enzymes (Extended Spectrum β-Lactamases/ESBL) which can degrade certain antibiotics (βlactam class) ( (Farhat et al, 2009 ;Bimanand et al, 2017)) and can form capsules (Khan et al, 2015), while P. aeruginosa can form biofilms (Alhazmi, 2015). Based on Podschun & Ullmann (1998) and Khan et al (2015), the common triggers of malignancy from these gram-negative bacteria are the presence of endotoxin (toxin which is an integral part of cell walls of gram-negative bacteria) and adhesin (extracellular matrix and plasma protein deposits used to attach to biomaterials).…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial Activity of Fungal Extract of The Aspergillus flavus from Hiri Island, North Maluku to Pathogenic Bacteria

et al. 2020

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Antibacterial compounds from sponge association fungi are one of the alternatives to search for new antibiotics against resistant bacteria. This study aims to explore the secondary metabolites potential from sponge association fungi as MDR pathogens antibacterial and to cultivate these fungi using a variety of cultivation media. TE-BO-09.1. Isolate can inhibit 3 bacteria, K. pneumoniae, B subtilis, and S. aureus, but is not able to inhibit E. coli and P. aeruginosa. Cultivation using standard media of Malt Extract Agar (MEA) and media modified from fish broth (M1, M2) and cassava infusion (M3, M4) produce secondary metabolites with varying quantities and antimicrobial activity. The inhibition zone of the produced extract with 500 μg/disc concentration ranged from 7.14 to 10.32 mm. The strongest potential was shown by ethyl acetate extract from isolates cultured with M2 (9 days cultivation), of which is able to produce 10.32 mm inhibition zones against S. aureus and methanol extracts from isolates cultured with M4 (6 days cultivation), and able to produce 10.05 mm inhibition zones against K. pneumoniae. In conclusion, the fungus Aspergillus flavus can be culture using fish broth added glucose and cassava infusion water added peptone media to potentially produce antibacterial compounds against MDR pathogens.

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Section: Resultsmentioning

confidence: 99%

Antimicrobial Activity of Fungal Extract of The Aspergillus flavus from Hiri Island, North Maluku to Pathogenic Bacteria

et al. 2020

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“…The anti-malarial drugs artemisinin and its derivatives (artesunate, dihydroartemisinin, artemether, and arteether) have been clinically used to treat malaria ( 18 , 19 , 30 ). Artesunate was also revealed to increase the susceptibility of various β-lactam antibiotics against E. coli by increasing antibiotics accumulation via inhibiting the efflux pump system, AcrAB-TolC, an essential and significant multi-drug efflux pump system within E. coli ( 31 , 32 ). Results showed that oxacillin had the greatest MIC, while ciprofloxacin had the lowest MIC, supported by those reported by CLSI ( 23 ).…”

Section: Discussionmentioning

confidence: 99%

Enhancing activity of β-lactam and fluoroquinolones antibiotics by artemisinin and its derivatives against MDR Escherichia coli

Haq

Guo²,

Aqib³

et al. 2022

Front. Vet. Sci.

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Artemisinin and its derivatives had played a biocidal role in biomedical remedies, while they were expected to enhance the activity of antibiotics against multiple drug-resistant (MDR) bacteria. The current study evaluated the interaction of artemisinin (ART), dihydroartemisinin (DHA), artesunate (AS), and artemisinic acid (AA) with β-lactam and fluoroquinolones antibiotics against Escherichia coli. Antibiotic strip test (E-test), Kirby Bauer's disc test (KB method), and broth microdilution method were adopted for susceptibility analysis, while the checkerboard method was applied to assess synergisms. ART, DHA, AS, and AA showed significantly enhanced antibacterial effects of β-lactam antibiotics against different strains of E. coli. The study showed ciprofloxacin to be most effective by presenting the least MIC (0.017125 ± 0.0022 μg/ml), while oxacillin was least effective (MIC 256 μg/ml) against E. coli. Synergism between AA and penicillin G (75%), ampicillin (25%), and oxacillin (50%) was observed in all isolates tested. AA and AS significantly decreased the MIC of ampicillin (−0.912 ± 0.908 μg/ml) and ciprofloxacin (−0.901 ± 0.893 g/ml), respectively. Artemisinin and its derivatives increased antibiotic accumulation within E. coli in a dose-dependent manner. The time-kill assay significantly reduced the bacterial number within 24 h of incubation. The study thus concludes greater room for improvement in enhancing the efficacy of antibiotics if used with artemisinin and its derivatives.

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“…The antimicrobial susceptibility of the clinical isolates and the standard strain to standard synthetic chemical antibiotics was assessed using rapid e-tests 4,5,7 , Briefly, the isolates were inoculated onto separate plates containing Mueller-Hinton agar and standard e-test plastic strips were infused with each antibiotic and incubated with the isolates at 37°C overnight to develop zones and ellipses. The interaction of the ellipse was taken as the minimum inhibitory concentration (MIC), whereas the zone indicated the susceptibility of the antibiotic to the bacterium.…”

Section: Antimicrobial Susceptibility Testsmentioning

confidence: 99%

“…Pseudomonas aeruginosa is a Gramnegative motile bacterium that is a major cause of community-acquired, and hospital-borne nosocomial infections 1,2 . The major concern about nosocomial infections with P. aeruginosa is that > 10% of infections are caused by multidrugresistant species 1,3,4 that arise through antibiotic abuse. Pseudomonas is only one among many bacterial species that have become resistant to multiple antibiotics, and the most resistant strain is P. aeruginosa 5 .…”

Section: Introductionmentioning

confidence: 99%

“…This bacterium causes frequent infections due to its natural adaptability and abundance, and it has emerged as the most important species with which to analyze multidrug resistance 1,6 . Here, we isolated multidrug-resistant strains from clinical specimens derived from patients with nosocomial infections, then compared the susceptibility of these strains to commercial synthetic antibiotics and to ethanoic acids prepared from organic apple cider, sugar molasses, dates, grapes, and grains using conventional standard microbiological techniques 4,7,8,9 . Pseudomonas aeruginosa ATCC27853 was the standard control strain.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of e-test Values for Standard Antibiotics and Conventional Antimicrobial Assay Values for Ethanoic Acids against Nosocomial Multidrug-resistant Pseudomonas aeruginosa

Maqbul¹,

Alshabi²,

Khan³

et al. 2020

J. Pure Appl. Microbiol.

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The present study aimed to determine the susceptibility of Pseudomonas aeruginosa strains isolated from patients with nosocomial infections to standard synthetic chemical antibiotics and organic ethanoic acids derived from local produce. The minimum inhibitory concentrations (MIC) of the standard synthetic antibiotics determined from standard e-test results and antibiotic sensitivity tests showed many multidrug-resistant strains among the isolates. We compared the susceptibility of these strains to organic ethanoic acids derived from different sources using standard microbiological assays. All strains of P. aeruginosa isolated from the patients were susceptible to the organic ethanoic acids with a satisfactory MIC and minimum bactericidal concentrations. Therefore, organic ethanoic acids were more effective against P. aeruginosa than standard synthetic antibiotics.

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E-test antibiotic susceptibility of E.coli strains isolated from hospital acquired infections of Imam Khomeini hospital, Ilam, Iran

Cited by 3 publications

References 4 publications

Antimicrobial Activity of Fungal Extract of The Aspergillus flavus from Hiri Island, North Maluku to Pathogenic Bacteria

Antimicrobial Activity of Fungal Extract of The Aspergillus flavus from Hiri Island, North Maluku to Pathogenic Bacteria

Enhancing activity of β-lactam and fluoroquinolones antibiotics by artemisinin and its derivatives against MDR Escherichia coli

Comparison of e-test Values for Standard Antibiotics and Conventional Antimicrobial Assay Values for Ethanoic Acids against Nosocomial Multidrug-resistant Pseudomonas aeruginosa

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