Earlier onset of DNA synthesis in mitogen-stimulated lymphocytes of cord blood than in lymphocytes of adult blood

Yoffey, J. M.; Ron, A.; Prindull, G.; Yaffe, P.

doi:10.1016/0090-1229(78)90145-9

Cited by 11 publications

(6 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The increased or normal numbers of B-lymphocytes in newborns [3,12] may in part explain the responses to ConA and PWM [9]. The capacity of lymphocytes to react to PHA has been demonstrated from the 12th week of gestational age [6], and the strong response to this mitogen in our newborns agrees with the observation of others [3,4,8,16].…”

Section: Discussionsupporting

confidence: 92%

Mitogen-induced multiplication of lymphocytes from newborn infants

1980

View full text Add to dashboard Cite

Marked lymphocyte responses to PHA, ConA and PWM were demonstrated in the first week of life. Prematurity up to 30 weeks gestational age did not influence the results. There were only small variations in the responses from day to day, no sex differences, and no differences between infants who were small for gestational age of appropriate weight. Newborn infants with high serum IgM-concentrations had reduced lymphocyte responses to ConA, possibly through the influence of ConA on B-lymphocytes. Infants of mothers who had received corticosteroids prior to delivery had lower mean responses to all 3 mitogens, but the differences between the steroid- and comparable non-steroid groups were not significant. The results suggest that newborn infants have functioning cellular immune mechanisms.

show abstract

Section: Discussionsupporting

confidence: 92%

Mitogen-induced multiplication of lymphocytes from newborn infants

1980

View full text Add to dashboard Cite

show abstract

“…The major finding is that the response of UCB lymphocytes to a B-cell mitogen, SpA CoI, was significantly low, although a good response to T-cell mitogens was observed. The fetal-lymphocyte response to PHA is known to appear as early as 10 to 14 weeks of gestation (8,17), but the kinetics of the neonatal-lymphocyte response to PHA is somewhat different from that of adult PB lymphocytes (19,21), that is, UCB lymphocytes respond to PHA more rapidly and begin DNA synthesis earlier than adult PB lymphocytes. This may be related to the fact that the UCB contains cells spontaneously synthesizing more DNA and are, therefore, able to enter the S phase rapidly (1,9,15,20).…”

Section: Discussionmentioning

confidence: 99%

“…In the earlier studies it was demonstrated that umbilical cord blood (UCB) contains lymphocytes that are morphologically different from adult cells and that these cells spontaneously synthesize more DNA than do adult peripheral lymphocytes (1,9,15,20). While the response of fetal lymphocytes to phytohemagglutinin (PHA) was detectable at 10 to 14 weeks of gestation (8,17), the kinetics of the response of newborn lymphocytes was different from that of adult peripheral blood (PB) lymphocytes (19,21). It has recently been reported that newborn T cells have some suppressive activity on the proliferative response of both maternal and unrelated adult lymphocytes (4,(11)(12)(13), suggesting their active participation as a possible mechanism in preventing the rejection of the fetus by the mother.…”

mentioning

confidence: 99%

Low Responsiveness of Human Neonatal Lymphocytes to a B‐Cell Mitogen, Staphylococcus aureus Cowan I (SpA CoI)

Kimura

Harada

Shioiri-Nakano

et al. 1980

Microbiology and Immunology

View full text Add to dashboard Cite

Responses of neonatal and adult lymphocytes to various mitogens were studied. Lymphocytes from umbilical cord blood (UCB) responded well to both phytohemagglutinin and concanavalin A, and also to pokeweed mitogen and Staphylococcus aureus Protein A. The responses of UCB lymphocytes to these mitogens were not significantly lower than those of adult peripheral blood lymphocytes (PBL). In contrast, UCB lymphocytes showed only a minimal response to killed Staphylococcus aureus Cowan I (SpA CoI), a potent B-cell mitogen for human PBL, although the proportion of B cells in UCB was not less than that in PBL. The low level of response of lymphocytes from UCB to SpA CoI was not ascribed to differences in dose response or kinetics. Purified B cells from UCB were not stimulated by SpA CoI either, suggesting that the low responsiveness was not due to the suppressive effect of T cells or macrophages, but to some intrinsic defect in B cells in UCB. These results suggest that the B cells in neonates may be more immature than the T cells.

show abstract

“…Recently, Yoffey et al (53) showed earlier onset of DNA synthesis in mitogen-stimulated lymphocytes of cord Blood than in lympho cytes of adult blood. These authors suggest that the more rapid response of neonatal lympho cytes may possess immunological significance as part of a cell surveillance mechanism of which there is particular need during a period of rapid growth.…”

Section: Discussionmentioning

confidence: 99%

T Cell Immune Function in Newborn Infants

Semenzato¹,

Piovesan²,

Amadori³

et al. 1980

Neonatology

View full text Add to dashboard Cite

T cell immune function in 20 newborn infants was investigated. Previous studies showing increased spontaneous transformation and higher ³H-thymidine incorporation at lower PHA concentration in newborn infants were confirmed. A net increase in the number of active E rosette-forming lymphocytes and a slight decrease in the percentage of total E rosette-forming cells was also found. Our results suggest the presence of a subpopulation of activated T lymphocytes in the peripheral blood of newborns during the first days of life. Possible mechanisms of this activation are discussed.

show abstract

Earlier onset of DNA synthesis in mitogen-stimulated lymphocytes of cord blood than in lymphocytes of adult blood

Cited by 11 publications

References 29 publications

Mitogen-induced multiplication of lymphocytes from newborn infants

Mitogen-induced multiplication of lymphocytes from newborn infants

Low Responsiveness of Human Neonatal Lymphocytes to a B‐Cell Mitogen, Staphylococcus aureus Cowan I (SpA CoI)

T Cell Immune Function in Newborn Infants

Contact Info

Product

Resources

About