Early-age thermal conditioning reduces uncoupling protein messenger RNA expression in pectoral muscle of broiler chicks at seven days of age

Taouis, Mohammed; Basilio, Vasco De; Mignon-Grasteau, Sandrine; Crochet, Sabine; Bouchot, C.; Bigot, Karine; Collin, Anne; Picard, Michel

doi:10.1093/ps/81.11.1640

Cited by 35 publications

(20 citation statements)

References 12 publications

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“…Interestingly, UCP3 mRNA levels may be upregulated by chronic heat exposure in pig muscle, but this effect seems depending on the energy intake levels (Katsumata et al, 2004). Moreover, our data contrasts previous results obtained in birds (Taouis et al, 2002;Mujahid et al, 2006;Collin et al, 2007). The duration of exposure, long-term (10 days) in our experiment versus acute effect in other experiments, may explain these disparate results.…”

Section: Regulation Of Leptin and Ucp Gene Expression In Chickenscontrasting

confidence: 96%

Acute cold‐ and chronic heat‐exposure upregulate hepatic leptin and muscle uncoupling protein (UCP) gene expression in broiler chickens

Dridi

Temim²,

Derouet³

et al. 2008

J. Exp. Zool.

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Emerging evidence showed that variations in environmental temperature affect both leptin and uncoupling protein (UCP) gene expression in mammals, whereas a little is known about such interactions in birds. Thus, we conducted the present study to investigate the influence of acute (2 hours) cold (4 degrees C) and chronic (10 days) heat (32 degrees C) exposure on hepatic leptin and muscle UCP gene expression in 5-wk-old broiler chickens. Both cold- and heat-exposure significantly (P < 0.05 to P < 0.001) upregulated hepatic leptin (by 35 and 46%, respectively) and muscle UCP mRNA levels (by 71 and 71%, respectively) compared to the thermoneutrality (22 degrees C). This result suggests that leptin and UCP may be involved in the thermoregulation response of chickens to extreme climate (cold and hot temperatures). The upregulation of hepatic leptin gene expression was accompanied by an increase in plasma leptin levels, indicating that leptin may be regulated at transcriptional level. The increase of leptin and UCP mRNA abundance, and leptinemia we report here were not related to plasma glucose or insulin levels. In conclusion, the exposure of broiler chickens to extreme ambient temperatures (cold and heat) increases hepatic leptin and muscle UCP gene expression.

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Section: Regulation Of Leptin and Ucp Gene Expression In Chickenscontrasting

confidence: 96%

Acute cold‐ and chronic heat‐exposure upregulate hepatic leptin and muscle uncoupling protein (UCP) gene expression in broiler chickens

Dridi

Temim²,

Derouet³

et al. 2008

J. Exp. Zool.

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“…Moreover, uncoupling protein (UCP) is a molecular determinant for the regulation of resting metabolic rate by thyroid hormone (de Lange et al, 2001), and expression of avUCP mRNA was significantly lower in thermal conditioned chicks than in control chicks. UCP mRNA expression in pectoral muscle and body temperature are quickly adjusted in chicks after early thermal conditioning (Taouis et al, 2002). Although we did not investigate effect of thermal conditioning on avUCP in muscle, these results suggest that reduced T 3 may be part of the mechanism associated with improved thermotolerance by early age heat conditioning.…”

Section: Discussionmentioning

confidence: 81%

Effect of Short-Term Thermal Conditioning on Physiological and Behavioral Responses to Subsequent Acute Heat Exposure in Chicks

et al. 2014

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It is known that thermal conditioning at an early age results in improved heat tolerance, and reduces mortality when re-exposed to heat in later life in chickens. However, the mechanism of thermal conditioning is not fully understood. The objective of this study was to investigate the effect of early thermal conditioning on physiological and behavioral responses in acute heat-exposed chicks. Six-day-old chicks (White Plymouth Rock) were exposed to high temperature at 40℃ for 3 h while control chicks were kept at 30℃. Four days after treatment, both groups were challenged to high temperature at 40℃ for 15 min. We found that the initiation times for behavioral responses (panting and wing-droop posture) in experienced chicks were later than those in control. At the end of heat-exposure treatment, the rectal temperature in experienced chicks was lower than that in control while there was no difference in respiration rate between the groups. Compared with control, experienced chicks had a lower level of plasma corticosterone. Gene expression levels of brain-derived neurotrophic factor, thyrotropin-releasing hormone, interleukin-6 and lipopolysaccharide-induced tumor necrosis factor were significantly lower in the brain of experienced chicks than in the control chicks. These results suggest that thermal conditioning may change response to subsequent heat exposure by altering the central thermoregulation system, resulting in an alleviation of heat stress.

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“…The mRNA expression of avUCP is also markedly increased in cold-acclimatized ducklings and in chickens from the Rϩ energy-inefficient laying strain (31), both of which present high heat production (1,11,14). In contrast, avUCP mRNA expression is reduced in early heat-conditioned chicks, which exhibit lower internal temperatures than nonconditioned chicks (37). It is likely that, as in mammals with UCP1, the enhancement of thermogenesis observed in T 3 -treated chickens (9) is partly mediated by increased expression of avUCP.…”

Section: Discussionmentioning

confidence: 99%

“…Recent results obtained in our laboratory (6) also suggest that the induction of avUCP mRNA expression in cold-exposed chicks is associated with increased plasma T 3 concentrations and heat production. Moreover, avUCP mRNA expression has been shown to be inhibited in chickens early conditioned to heat (37), characterized by low plasma T 3 concentrations (39). However, the influence of thyroid hormones on avUCP expression in chickens has not previously been clearly demonstrated.…”

mentioning

confidence: 99%

Thyroid status, but not insulin status, affects expression of avian uncoupling protein mRNA in chicken

Collin

Taouis

Buyse

et al. 2003

American Journal of Physiology-Endocrinology and Metabolism

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Decuypere. Thyroid status, but not insulin status, affects expression of avian uncoupling protein mRNA in chicken. Am J Physiol Endocrinol Metab 284: E771-E777, 2003. First published December 10, 2002 10.1152/ajpendo.00478.2002The aim of this study was to investigate the hormonal regulation of the avian homolog of mammalian uncoupling protein (avUCP) by studying the impact of thyroid hormones and insulin on avUCP mRNA expression in chickens (Gallus gallus). For 3 wk, chicks received either a standard diet (control group), or a standard diet supplemented with triiodothyronine (T 3; T3 group) or with the thyroid gland inhibitor methimazole (MMI group). A fourth group received injections of the deiodinase inhibitor iopanoic acid (IOP group). During the 4th wk of age, all animals received two daily injections of either human insulin or saline solution. The results indicate a twofold overexpression of avUCP mRNA in gastrocnemius muscle of T3 birds and a clear downregulation (Ϫ74%) in MMI chickens compared with control chickens. Insulin injections had no significant effect on avUCP mRNA expression in chickens. This study describes for the first time induction of avUCP mRNA expression by the thermogenic hormone T 3 in chickens and supports a possible involvement of avUCP in avian thermogenesis. thyroid hormones; thermogenesis; muscle IN MAMMALS, mitochondrial uncoupling proteins (UCPs) are known to uncouple phosphorylation from oxidation and, hence, to be involved in energy metabolism. Brown fat UCP1 has also been reported to be involved in heat production (for review see Ref. 32). The impact of thyroid hormones on UCP expression is well documented (16,23,25), and UCP3 could be one mediator of the thermogenic effect of triiodothyronine (T 3 ) in mammalian skeletal muscle (10). However, the implication of UCP3 in thermogenesis in mammals is controversial (17, 33).As in mammals, T 3 has been reported to have a role in thermoregulatory mechanisms in birds by stimulating heat production (9, 34). However, the involvement of uncoupling mechanisms in such regulation is still unclear. A recent study (31) showed that a UCP homolog called avian (av)UCP is expressed in chicken and duckling muscle. The authors suggest that avUCP is structurally close to mammalian UCP2 and UCP3 but that its function could be nearer to that of UCP1 (expressed exclusively in brown adipose tissue) in mammals. Indeed, the avUCP messenger is overexpressed in cases of cold acclimatization in ducklings and in cockerels from the Rϩ line presenting a high diet-induced thermogenesis (31). Recent results obtained in our laboratory (6) also suggest that the induction of avUCP mRNA expression in cold-exposed chicks is associated with increased plasma T 3 concentrations and heat production. Moreover, avUCP mRNA expression has been shown to be inhibited in chickens early conditioned to heat (37), characterized by low plasma T 3 concentrations (39). However, the influence of thyroid hormones on avUCP expression in chickens has not previously been clearly demonstr...

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Early-age thermal conditioning reduces uncoupling protein messenger RNA expression in pectoral muscle of broiler chicks at seven days of age

Cited by 35 publications

References 12 publications

Acute cold‐ and chronic heat‐exposure upregulate hepatic leptin and muscle uncoupling protein (UCP) gene expression in broiler chickens

Acute cold‐ and chronic heat‐exposure upregulate hepatic leptin and muscle uncoupling protein (UCP) gene expression in broiler chickens

Effect of Short-Term Thermal Conditioning on Physiological and Behavioral Responses to Subsequent Acute Heat Exposure in Chicks

Thyroid status, but not insulin status, affects expression of avian uncoupling protein mRNA in chicken

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