Early bone apposition in vivo on plasma-sprayed and electrochemically deposited hydroxyapatite coatings on titanium alloy

Wang, Hao; Eliaz, Noam; Xiang, Zhou; Hsu, Hu‐Ping; Spector, Myron; Hobbs, Linn W.

doi:10.1016/j.biomaterials.2006.03.034

Cited by 204 publications

(139 citation statements)

References 35 publications

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“…While model biomaterial surfaces to control ligand presentation (e.g., self-assembled monolayers on gold or silicon) have been extensively studied, the development of robust coating technologies for tunable presentation of bioactive factors on materials approved for biomedical implantation has been particularly challenging. Recent bioactive implant surface treatments on Ti, including porous hydroxyapatite, collagen I, and calcium phosphate co-precipitated with various other biological ligands, have augmented aspects of bone healing compared to unmodified Ti in various animal models [27][28][29]. However, these strategies lack control over protein adsorption, ligand presentation and density, and surface stability.…”

Section: Discussionmentioning

confidence: 99%

The effect of integrin-specific bioactive coatings on tissue healing and implant osseointegration

et al. 2008

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Implant osseointegration, defined as bone apposition and functional fixation, is a requisite for clinical success in orthopaedic and dental applications, many of which are restricted by implant loosening. Modification of implants to present bioactive motifs such as the RGD cell-adhesive sequence from fibronectin (FN) represents a promising approach in regenerative medicine. However, these biomimetic strategies have yielded only marginal enhancements in tissue healing in vivo. In this study, clinical-grade titanium implants were grafted with a non-fouling oligo(ethylene glycol)-substituted polymer coating functionalized with controlled densities of ligands of varying specificity for target integrin receptors. Biomaterials presenting the α 5 β 1 -integrin-specific FN fragment FNIII 7-10 enhanced osteoblastic differentiation in bone marrow stromal cells compared to unmodified titanium and RGD-presenting surfaces. Importantly, FNIII 7-10 -functionalized titanium significantly improved functional implant osseointegration compared to RGD-functionalized and unmodified titanium in vivo. This study demonstrates that bioactive coatings that promote integrin binding specificity regulate marrow-derived progenitor osteoblastic differentiation and enhance healing responses and functional integration of biomedical implants. This work identifies an innovative strategy for the rational design of biomaterials for regenerative medicine.

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Section: Discussionmentioning

confidence: 99%

The effect of integrin-specific bioactive coatings on tissue healing and implant osseointegration

et al. 2008

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“…[4][5][6][7] Among these methods, chemical treatment is a relatively simple and effective technique and has been widely investigated. [8][9][10][11] In previous studies, a type of nanorod structure with a phase composition of anatase and rutile has been fabricated on a titanium surface using different chemical processes.…”

Section: Introductionmentioning

confidence: 99%

Effects of a hybrid micro/nanorod topography-modified titanium implant on adhesion and osteogenic differentiation in rat bone marrow mesenchymal stem cells

Zhang¹,

Li²,

Huang³

et al. 2013

IJN

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Background and methods:Various methods have been used to modify titanium implant surfaces with the aim of achieving better osseointegration. In this study, we fabricated a clustered nanorod structure on an acid-etched, microstructured titanium plate surface using hydrogen peroxide. We also evaluated biofunctionalization of the hybrid micro/nanorod topography on rat bone marrow mesenchymal stem cells. Scanning electron microscopy and x-ray diffraction were used to investigate the surface topography and phase composition of the modified titanium plate. Rat bone marrow mesenchymal stem cells were cultured and seeded on the plate. The adhesion ability of the cells was then assayed by cell counting at one, 4, and 24 hours after cell seeding, and expression of adhesion-related protein integrin β1 was detected by immunofluorescence. In addition, a polymerase chain reaction assay, alkaline phosphatase and Alizarin Red S staining assays, and osteopontin and osteocalcin immunofluorescence analyses were used to evaluate the osteogenic differentiation behavior of the cells. Results:The hybrid micro/nanoscale texture formed on the titanium surface enhanced the initial adhesion activity of the rat bone marrow mesenchymal stem cells. Importantly, the hierarchical structure promoted osteogenic differentiation of these cells. Conclusion: This study suggests that a hybrid micro/nanorod topography on a titanium surface fabricated by treatment with hydrogen peroxide followed by acid etching might facilitate osseointegration of a titanium implant in vivo.

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“…8,9 Because the topography of the implant surface can serve as a powerful signaling cue for the attached cells, various approaches have been developed for fabrication of a series of improved implant surfaces, amongst which nanostructure designs have been of great interest, due to their favorable biofunctionalization. 9,10 Nanostructures located on titanium and its alloys can be synthesized by various techniques, such as chemical treatment, plasma spraying, 11 anodic oxidation, 12 physical vapor deposition, 13 and chemical vapor deposition. 14 Chemical treatment is a simple and effective approach and has been widely used in recent years.…”

Section: Introductionmentioning

confidence: 99%

Biofunctionalization of a titanium surface with a nano-sawtooth structure regulates the behavior of rat bone marrow mesenchymal stem cells

Zhang¹,

Li²,

Liu³

et al. 2012

IJN

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Background:The topography of an implant surface can serve as a powerful signaling cue for attached cells and can enhance the quality of osseointegration. A series of improved implant surfaces functionalized with nanoscale structures have been fabricated using various methods. Methods: In this study, using an H 2 O 2 process, we fabricated two size-controllable sawtooth-like nanostructures with different dimensions on a titanium surface. The effects of the two nanosawtooth structures on rat bone marrow mesenchymal stem cells (BMMSCs) were evaluated without the addition of osteoinductive chemical factors. Results: These new surface modifications did not adversely affect cell viability, and rat BMMSCs demonstrated a greater increase in proliferation ability on the surfaces of the nanosawtooth structures than on a control plate. Furthermore, upregulated expression of osteogenicrelated genes and proteins indicated that the nano-sawtooth structures promote osteoblastic differentiation of rat BMMSCs. Importantly, the large nano-sawtooth structure resulted in the greatest cell responses, including increased adhesion, proliferation, and differentiation. Conclusion: The enhanced adhesion, proliferation, and osteogenic differentiation abilities of rat BMMSCs on the nano-sawtooth structures suggest the potential to induce improvements in bonetitanium integration in vivo. Our study reveals the key role played by the nano-sawtooth structures on a titanium surface for the fate of rat BMMSCs and provides insights into the study of stem cellnanostructure relationships and the related design of improved biomedical implant surfaces.

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Early bone apposition in vivo on plasma-sprayed and electrochemically deposited hydroxyapatite coatings on titanium alloy

Cited by 204 publications

References 35 publications

The effect of integrin-specific bioactive coatings on tissue healing and implant osseointegration

The effect of integrin-specific bioactive coatings on tissue healing and implant osseointegration

Effects of a hybrid micro/nanorod topography-modified titanium implant on adhesion and osteogenic differentiation in rat bone marrow mesenchymal stem cells

Biofunctionalization of a titanium surface with a nano-sawtooth structure regulates the behavior of rat bone marrow mesenchymal stem cells

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