Early born lineage of retinal neurons express class III β-tubulin isotype

Sharma, Rajesh Kumar; Netland, Peter A.

doi:10.1016/j.brainres.2007.07.090

Cited by 69 publications

(55 citation statements)

References 14 publications

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“…It is noteworthy that the expression of the fusion protein was not solely located in the ganglion cells. Consistent with other report which used Tuj-1 antibody to determine Class III β-tubulin expression in the retina [12], we found some photoreceptor cells, certain amacrine cells ( Figure 2E, arrow), some horizontal cells and bipolar cells also express the fusion protein, although the latter two showed a low fluorescent signal ( Figure 2E). …”

Section: Tubb3-mcherry Fusion Protein Is Expressed In Various Tissuessupporting

confidence: 92%

A bacterial artificial chromosome transgenic mouse model for visualization of neurite growth

Tao

Chen

Sun

et al. 2015

Sci. China Life Sci.

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Class III β-tubulin (Tubb3) is a component of the microtubules in neurons and contributes to microtubule dynamics that are required for axon outgrowth and guidance during neuronal development. We here report a novel bacterial artificial chromosome (BAC) transgenic mouse line that expresses Class III β-tubulin fused to mCherry, an improved monomeric red fluorescent protein, for the visualization of microtubules during neuronal development. A BAC containing Tubb3 gene was modified by insertion of mCherry complementary DNA downstream of Tubb3 coding sequence via homologous recombination. mCherry fusion protein was expressed in the nervous system and testis of the transgenic animal, and the fluorescent signal was observed in the neurons that located in the olfactory bulb, cerebral cortex, hippocampal formation, cerebellum, as well as the retina. Besides, Tubb3-mCherry fusion protein mainly distributed in neurites and colocalized with endogenous Class III β-tubulin. The fusion protein labels Purkinje cell dendrites during cerebellar circuit formation. Therefore, this transgenic line might be a novel tool for scientific community to study neuronal development both in vitro and in vivo.

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Section: Tubb3-mcherry Fusion Protein Is Expressed In Various Tissuessupporting

confidence: 92%

A bacterial artificial chromosome transgenic mouse model for visualization of neurite growth

Tao

Chen

Sun

et al. 2015

Sci. China Life Sci.

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“…The expression of these bHLH factors was not initiated at E14.5 (Fig. 4H′-L′), suggesting that not only proliferation but also the retina-specific differentiation program was severely affected in the absence of Pax6.To test whether general neuronal differentiation took place in the mutants, retinae were stained with antibody against the pan-neuronal marker acetylated beta III tubulin (Tuj1, also known as Tubb3), which marks differentiating cells and reveals formation of the differentiated cell layer (DCL) (Sharma and Netland, 2007;Sigulinsky et al, 2008). Although strong Tuj1 staining was observed in the DCL of wild-type retinae at E14.5 and E16.5 (Fig.…”

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confidence: 99%

Stage-dependent requirement of neuroretinal Pax6 for lens and retina development

Klimova

Kozmík

2014

Development

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The physical contact of optic vesicle with head surface ectoderm is an initial event triggering eye morphogenesis. This interaction leads to lens specification followed by coordinated invagination of the lens placode and optic vesicle, resulting in formation of the lens, retina and retinal pigmented epithelium. Although the role of Pax6 in early lens development has been well documented, its role in optic vesicle neuroepithelium and early retinal progenitors is poorly understood. Here we show that conditional inactivation of Pax6 at distinct time points of mouse neuroretina development has a different impact on early eye morphogenesis. When Pax6 is eliminated in the retina at E10.5 using an mRx-Cre transgene, after a sufficient contact between the optic vesicle and surface ectoderm has occurred, the lens develops normally but the pool of retinal progenitor cells gradually fails to expand. Furthermore, a normal differentiation program is not initiated, leading to almost complete disappearance of the retina after birth. By contrast, when Pax6 was inactivated at the onset of contact between the optic vesicle and surface ectoderm in Pax6Sey/flox embryos, expression of lens-specific genes was not initiated and neither the lens nor the retina formed. Our data show that Pax6 in the optic vesicle is important not only for proper retina development, but also for lens formation in a non-cell-autonomous manner. KEY WORDS: Pax6, Retinal progenitor, mRx-Cre, Lens induction INTRODUCTIONProper eye development is dependent on the coordinated formation of two main tissues in the eye: the retina and the lens. Vertebrate eye development begins with invagination of the optic vesicle (OV) toward the lens-competent head surface ectoderm (SE). As OV contacts SE, a series of reciprocal inductive signals elicit formation of the lens placode (LP) and subsequent invagination of both LP and OV to form a two-layered optic cup (OC), with retinal pigmented epithelium (RPE) surrounding the retina (reviewed by Fuhrmann, 2010; Chow and Lang, 2001;Ogino et al., 2012). Genetic studies have identified multiple transcription factors and signaling pathways interacting in a complex network orchestrating early eye development (reviewed by Fuhrmann, 2010; Chow and Lang, 2001;Ogino et al., 2012;Xie and Cvekl, 2011). Among the signaling pathways, BMP (Furuta and Hogan, 1998;Rajagopal et al., 2009;Sjödal et al., 2007;Wawersik et al., 1999) and FGF (Faber et al., 2001;Garcia et al., 2011;Gotoh et al., 2004;Pan et al., 2006) were found to be essential for lens induction and coordinated OV-to-OC transition, as severe eye defects are associated with their inactivation. RESEARCH ARTICLEInstitute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, 14420 Prague 4, Czech Republic. At the time the LP is formed, the dorsal region of the OV becomes specified to the retina and is populated with mitotically active retinal progenitor cells (RPCs) (Fuhrmann, 2010;Levine and Green, 2004). Lineage-tracing studies have shown that RPCs a...

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“…Others have shown that a co-localisation pattern also exists for Thy-1 and NeuN whereby 95.4% of thy-1-CFP cells in the RGCL also labelled with NeuN (Raymond et al, 2008). In addition, we observed a greater It should be noted that in rodents βIII tubulin antibodies can cross react with ligands expressed on amacrine cells (Sharma and Netland, 2007). Similarly, probing for NeuN in rats revealed significant INL staining, likely to be that of amacrine cells (Bull et al, 2011;Johnson et al, 2014).…”

Section: Rgc Markers In the Human Retinamentioning

confidence: 58%

Human organotypic retinal cultures (HORCs) as a chronic experimental model for investigation of retinal ganglion cell degeneration

Osborne

Hopes

Wright

et al. 2016

Experimental Eye Research

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There is a growing need for models of human diseases that utilise native, donated human tissue in order to model disease processes and develop novel therapeutic strategies. In this paper we assessed the suitability of aged human retinal explants as a potential model of chronic retinal ganglion cell (RGC) degeneration. Our results confirmed that RGC markers commonly used in rodent studies (NeuN, βIII Tubulin and Thy-1) were appropriate for labelling human RGCs and followed the expected differential expression patterns across, as well as throughout, the macular and paramacular regions of the retina. Furthermore, we showed that neither donor age nor post-mortem time (within 24 hours) significantly affected the initial expression levels of RGC markers. In addition, the feasibility of using human post mortem donor tissue as a long-term model of RGC degeneration was determined with RGC protein being detectable up to 4 weeks in culture with an associated decline in RGC mRNA and significant, progressive, apoptotic labelling of NeuN + cells. Differences in RGC apoptosis might have been influenced by medium compositions indicating that media constituents could play a role in supporting axotomised RGCs. We propose that using ex vivo human explants may prove to be a useful model for testing the effectiveness of neuroprotective strategies.

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Early born lineage of retinal neurons express class III β-tubulin isotype

Cited by 69 publications

References 14 publications

A bacterial artificial chromosome transgenic mouse model for visualization of neurite growth

A bacterial artificial chromosome transgenic mouse model for visualization of neurite growth

Stage-dependent requirement of neuroretinal Pax6 for lens and retina development

Human organotypic retinal cultures (HORCs) as a chronic experimental model for investigation of retinal ganglion cell degeneration

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