Early bovine embryos regulate oviduct epithelial cell gene expression during in vitro co-culture

Schmaltz-Panneau, Barbara; Cordova, Amanda; Dhorne–Pollet, Sophie; Hennequet-Antier, Christelle; Uzbekova, Svetlana; Martinot, Emmanuelle; Doret, Sarah; Martin, Patrice; Mermillod, Pascal; Locatelli, Yann

doi:10.1016/j.anireprosci.2014.06.022

Cited by 64 publications

(45 citation statements)

References 100 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The same group subsequently described differential gene expression in bovine oviduct epithelial cells at oestrus and dioestrus (Day 12) [37], although the physiological relevance of this observation is questionable given that the embryo leaves the oviduct by Day 4 in cattle. Others have described embryo-induced alterations in the gene expression profile of bovine oviduct epithelial cells in vitro following culture with or without embryos [38,39]. While these results are certainly interesting, they invariably involve the culture of groups rather than single embryos and typically they examine gene expression in the oviduct cells after culture of embryos for 7 to 8 days to the blastocyst stage.…”

Section: Discussionmentioning

confidence: 99%

Oviduct-Embryo Interactions in Cattle: Two-Way Traffic or a One-Way Street?1

Maillo

Gaora

Forde

et al. 2015

Biology of Reproduction

View full text Add to dashboard Cite

This study examined the effect of the presence of single or multiple embryos on the transcriptome of the bovine oviduct. In experiment 1, cyclic (nonbred, n = 6) and pregnant (artificially inseminated, n = 11) heifers were slaughtered on Day 3 after estrus, and the ampulla and isthmic regions of the oviduct ipsilateral to the corpus luteum were separately flushed. Oviductal epithelial cells from the isthmus region, in which all oocytes/embryos were located, were snap-frozen for microarray analysis. In experiment 2, heifers were divided into cyclic (nonbred, n = 6) or pregnant (multiple embryo transfer, n = 10) groups. In vitro-produced presumptive zygotes were transferred endoscopically to the ipsilateral oviduct on Day 1.5 postestrus (n = 50 zygotes/heifer). Heifers were slaughtered on Day 3, and oviductal isthmus epithelial cells were recovered for RNA sequencing. Microarray analysis in experiment 1 failed to detect any difference in the transcriptome of the oviductal isthmus induced by the presence of a single embryo. In experiment 2, following multiple embryo transfer, RNA sequencing revealed 278 differentially expressed genes, of which 123 were up-regulated and 155 were down-regulated in pregnant heifers. Most of the down-regulated genes were related to immune function. In conclusion, the presence of multiple embryos in the oviduct resulted in the detection of differentially expressed genes in the oviductal isthmus; failure to detect changes in the oviduct transcriptome in the presence of a single embryo may be due to the effect being local and undetectable under the conditions of this study.

show abstract

Section: Discussionmentioning

confidence: 99%

Oviduct-Embryo Interactions in Cattle: Two-Way Traffic or a One-Way Street?1

Maillo

Gaora

Forde

et al. 2015

Biology of Reproduction

View full text Add to dashboard Cite

show abstract

“…EPSTI1 was reported overexpressed in an in vitro model of maternal environment represented by the coculture of bovine embryos with oviduct epithelial cells. 11 Notably, this study also found PKR to be upregulated and to presumably serve as a feedback mechanism to halt STAT1-dependent production of the other interferon response genes. This raises a possible immune dampening feedback mechanism for EPSTI1 as well.…”

mentioning

confidence: 59%

“…In addition to its role in cancer, EPSTI1 has also been implicated in immune privilege, 1,7,8 autoimmune disorders, 9,10 fetal development, 11,12 apoptosis regulation, 13 adipose tissue metabolism, 14,15 and hepatitis virus infection. 16,17 Importantly, all these biological processes appear to be associated with the stromal or inflammatory factors, such as interferon alpha (IFN-α) and transforming growth factor beta (TGF-β).…”

Section: Introductionmentioning

confidence: 99%

Implications of Epithelial–Stromal Interaction 1 in Diseases Associated with Inflammatory Signaling

Gray

Zhao

2016

Cell Communication Insights

View full text Add to dashboard Cite

Epithelial-stromal interaction 1 (EPSTI1) was initially identified as an induced gene in breast cancer epithelial cells by cocultured stromal fibroblasts. This discovery led to further investigation and understanding of the role of EPSTI1 in cancer. Aberrant elevation of EPSTI1 occurs primarily in invasive breast cancer epithelial cells. Forced overexpression of EPSTI1 in noninvasive cancer cells can substitute for the stromal fibroblasts. EPSTI1 was further implicated in cancer by our most recent study that identified it as one of the few most upregulated genes in human breast cancer by Krüppel-like factor 8 (KLF8), a pro-cancerous transcription factor in many cancer types. Our study also demonstrated that EPSTI1 interacts with valosin-containing protein to promote the degradation of nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB) inhibitor alpha, leading to the activation and nuclear translocation of NF-κB. Additionally, EPSTI1 was shown to inhibit apoptosis by inactivating caspase 8. Studies on hepatitis C and E viruses have indicated that EPSTI1 plays a role in inhibition of the viral replication by promoting the expression of protein kinase R or protein kinase RNA-activated, a viral response gene, suggesting a role of EPSTI1 in immune response. Interestingly, in addition to transducing stromal signals, EPSTI1 has been implicated in immune privilege and autoimmune diseases, including systemic lupus erythematosus, rheumatoid arthritis, and histiocytic necrotizing lymphadenitis. This review seeks to comb EPSTI1-related studies as it was cloned a dozen years ago with a particular focus on the mechanisms of its regulation and signaling, as well as its potential roles in the diseases.

show abstract

“…Recently, Schmaltz-Panneau et al (2014) described transcriptome changes in BOEC related to the presence of bovine embryos. BOEC are usually obtained from oviducts of slaughtered heifers or cows.…”

Section: Oviductal Environment and In Vitro Modelsmentioning

confidence: 99%

“…BOEC at oestrus have been successfully used as in vitro model simulating embryo maternal interactions (Rief et al, 2002). Recently, Cordova et al (2014) used oestrus-metoestrus (day 0-3) BOEC for early (day 1-4) or late (day 4-7) embryo co-culture showing that the presence of the cells during the first four days of development, which correspond to the presence of embryos in the oviduct in vivo, accelerated the kinetics of blastocyst development and induced changes in genes involved in epigenetic control. The positive effects of these cells on the embryos are attributed to embryotrophic substances, such as growth factors secreted by the cells (Nancarrow and Hill, 1994;Vanroose et al, 2001).…”

Section: Oviductal Environment and In Vitro Modelsmentioning

confidence: 99%

Role of the oviduct and oviduct-derived products in ruminant embryo development

Rizos¹,

Maillo²,

Lonergan³

2016

Anim Reprod

View full text Add to dashboard Cite

The fact that embryos can be obtained in vitro undermines the role of the oviduct. However, it has been demonstrated that when in vitro produced bovine zygotes are cultured in the oviduct of sheep, cattle or mice the embryo quality is improved compared to the embryos produced in vitro. Thus the oviduct is not simply a passive organ required only for transporting the embryo to the uterus but also provides a suitable microenvironment for the early embryo. The study of physiological mechanisms and interactions between the embryo and the oviductal environment is essential to understand the correct processes of early embryo developmental. This knowledge can be used to improve current in vitro procedures providing high quality embryos capable of continued development and implantation, and resulting in viable births.

show abstract

Early bovine embryos regulate oviduct epithelial cell gene expression during in vitro co-culture

Cited by 64 publications

References 100 publications

Oviduct-Embryo Interactions in Cattle: Two-Way Traffic or a One-Way Street?1

Oviduct-Embryo Interactions in Cattle: Two-Way Traffic or a One-Way Street?1

Implications of Epithelial–Stromal Interaction 1 in Diseases Associated with Inflammatory Signaling

Role of the oviduct and oviduct-derived products in ruminant embryo development

Contact Info

Product

Resources

About

Early bovine embryos regulate oviduct epithelial cell gene expression during in vitro co-culture

Cited by 64 publications

References 100 publications

Oviduct-Embryo Interactions in Cattle: Two-Way Traffic or a One-Way Street?1

Oviduct-Embryo Interactions in Cattle: Two-Way Traffic or a One-Way Street?1

Implications of Epithelial&ndash;Stromal Interaction 1 in Diseases Associated with Inflammatory Signaling

Role of the oviduct and oviduct-derived products in ruminant embryo development

Contact Info

Product

Resources

About

Implications of Epithelial–Stromal Interaction 1 in Diseases Associated with Inflammatory Signaling