Early Detection of Lewis Lung Carcinoma Tumor Control by Irradiation Using Diffusion-Weighted and Dynamic Contrast-Enhanced MRI

Abstract

PurposeTo investigate the correlation between diffusion-weighted (DW) and dynamic contrast-enhanced (DCE) magnetic resonance imaging (MRI) derived parameters and radioresponsiveness of Lewis lung carcinoma (LLC) tumor.Materials and MethodsLLC tumor growth in C57BL/6 mouse limb was used for the experiment. The tumors were irradiated with 10 Gy×5, or 30 Gy×2 vs. sham irradiation. Fourteen tumors were subjected to DW-MRI and DCE-MRI pre-radiotherapy and weekly imaging after radiotherapy. The temporal changes in apparent diffusion coefficient (ADC) and DCE-MRI derived parameters (Ktrans, kep, ve, and vp) were correlated with tumor size, and were histologically compared with CD31 staining of resected tumors.ResultsThe 10 Gy×5 dose inhibited tumor growth for a week, while 30 Gy×2 controlled tumor growth for a 3-week observation period. One week after radiotherapy (week 2), irradiated tumors showed significantly higher values of ADC than untreated ones (10 Gy×5, p = 0.004; 30 Gy×2, p = 0.01). Significantly higher values of ve were shown earlier by 30 Gy×2 vs. sham (p = 0.01) and 10 Gy×5 vs. sham irradiation (p = 0.05). Sustained higher ve from 10 Gy×5 compared to sham irradiated tumors was evident at week 3 (p = 0.016) and week 4 (p = 0.046). A 13.8% early increase in ADC for 30 Gy×2 tumor group (p = 0.002) and a 16.5% increase for 10 Gy×5 group were noted (p = 0.01) vs. sham irradiation (which showed a 2.2% decrease). No differences were found for Ktrans, kep, or vp. Both radiotherapy groups demonstrated significant reduction in microvessel counts.ConclusionEarly increase in ADC and ve correlated with tumor control by irradiation.

Response assessment of stereotactic body radiation therapy using dynamic contrast‐enhanced integrated MR‐PET in non‐small cell lung cancer patients

“…DCE-MRI has been widely used to inform pharmacodynamic modeling that evaluates vascular perfusion and permeability in patients with different cancers. 8,[17][18][19][20] Our study demonstrated significant decreases in the SUV max , K trans mean, K trans SD, k ep mean, k ep SD, and v p SD. K trans characterizes the diffusive transport of low-molecular weight gadolinium chelates across the capillary endothelium, and it is regarded as a biomarker to assess antiangiogenic and antivascular therapies; k ep indicates the rate constant (k e P 5 K trans / v e ); v p indicates the plasma volume, and v e indicates extracellular volume.…”

“…DCE‐MRI has been widely used to inform pharmacodynamic modeling that evaluates vascular perfusion and permeability in patients with different cancers . Our study demonstrated significant decreases in the SUV max , K trans mean, K trans SD, k ep mean, k ep SD, and v p SD.…”

Correlation of quantitative parameters of magnetic resonance perfusion‐weighted imaging with vascular endothelial growth factor, microvessel density and hypoxia‐inducible factor‐1α in nasopharyngeal carcinoma: Evaluation on radiosensitivity study

“…A study of Zheng et al demonstrated that a higher Kep was associated with lower, concurrent chemoradiotherapy and there was a significant difference between the groups. Furthermore, it revealed that Ve was significantly increased at dose of 10 Gy and 30 Gy in animal tests of lung cancer . Kim found that Ve was positively correlated with tumour regression rate in the cervical cancer.…”

18F-FDG PET and DCE kinetic modeling and their correlations in primary NSCLC: First voxel-wise correlative analysis of human simultaneous [18F]FDG PET-MRI data

“…The selection of the correct model for the right tumor is limited by what is named the "mixed tissue conundrum" [69], and remains mainly driven by both its bias-variance tradeoff and clinical relevance. In this way, DCE Tofts models have become standards in oncology [70,71], and have shown preclinical and clinical relevance in lung cancer speci cally [15,16,72]. The dual AIF, however, has never been validated for dynamic PET analyses, and therefore cannot be considered as a reference.…”

“…In the case of gadolinium (Gd), the K trans , v e , K ep (the K trans to v e ratio) and v p microparameters re ect the perfusion, permeability and microvascular density properties of the tumor [13]. Previous oncological studies showed DCE microparameters to be signi cant predictors of response to treatment in several malignancies [14][15][16][17], including NSCLC [18].…”