Early Detection of SARS-CoV-2 Seroconversion in Humans with Aggregation-Induced Near-Infrared Emission Nanoparticle-Labeled Lateral Flow Immunoassay

Conspectus Infectious diseases by pathogenic microorganisms are one of the leading causes of mortality worldwide. Healthcare and socio-economic development have been seriously affected for different civilizations because of bacterial and viral infections. According to the Centers for Disease Control and Prevention (CDC), pandemic in 1918 by the Influenza A virus of the H1N1 subtype was responsible for 50 to 100 million deaths worldwide. Similarly, the Asian flu pandemic in 1957, Hong Kong flu in 1968, and H1N1pdm09 flu pandemic in 2009 were responsible for more than 1 million deaths across the globe each time. As per the World Health Organization (WHO), the current pandemic by coronavirus disease 2019 (COVID-19) due to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus is responsible for more than 4.8 M death worldwide until now. Since the gold standard polymerase chain reaction (PCR) test is more time-consuming, the health care system cannot test all symptomatic and asymptomatic Covid patients every day, which is extremely important to tackle the outbreak. One of the significant challenges during the current pandemic is developing mass testing tools, which is critical to control the virus spread in the community. Therefore, it is highly desirable to develop advanced material-based approaches that can provide a rapid and accurate diagnosis of COVID-19, which will have the capability to save millions of human lives. Aiming for the targeted diagnosis of deadly virus, researchers have developed nanomaterials with various sizes, shapes, and dimensions. These nanomaterials have been used to identify biomolecules via unique optical, electrical, magnetic, structural, and functional properties, which are lacking in other materials. Despite significant progress, nanomaterial-based diagnosis of biomolecules is still facing several obstacles due to low targeting efficiency and nonspecific interactions. To overcome these problems, the bioconjugated nanoparticle has been designed via surface coating with polyethylene glycol (PEG) and then conjugated with antibodies, DNA, RNA, or peptide aptamers. Therefore, the current Account summarizes an overview of the recent advances in the design of bioconjugated nanomaterial-based approached as effective diagnosis of the SARS-CoV-2 virus and the SARS-CoV-2 viral RNA, antigen, or antibody, with a particular focus on our work and other’s work related to this subject. First, we present how to tailor the surface functionalities of nanomaterials to achieve bioconjugated material for targeted diagnosis of the virus. Then we review the very recent advances in the design of antibody/aptamer/peptide conjugated nanostructure, which represent a powerful platform for naked-eye colorimetric detection via plasmonic nanoparticles. We then discuss nanomaterial-based surface-enhanced Raman scattering (SERS) spectroscopy, which has the capability for very low-level fingerprint identification of virus, antigen, and antibody via graphene, plasmonic nanopa...

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Section: Cnt and Fluorescence Nanoparticle-based Sensor For Sars-cov-2 Sensingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bioconjugated Nanomaterial for Targeted Diagnosis of SARS-CoV-2

et al. 2022

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“…Therefore, it is visionary and pragmatic to prepare rapid detection kits for pathogenic microorganisms through chemical synthesis and nanoscale preparation during sudden outbreaks of novel epidemics and pathogen-related major biosafety threats, which can achieve valid results within minutes. As shown in Figure 3 , Chen et al developed a test kit for SARS-COV-2 based on aggregation induced emission (AIE) materials [47] . The AIE dye and virus antigen on the surface of the nanoparticle were fabricated for early detection of the virus.…”

Section: Possible Application Of Biosafety Chemistry and Biosafety Materialsmentioning

confidence: 99%

Biosafety chemistry and biosafety materials: A new perspective to solve biosafety problems

Ding

Zhou

et al. 2022

Biosafety and Health

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The Novel Coronavirus Disease (COVID-19) has rapidly swept around the globe since its first emergence near 2020. However, people have failed to fully understand its origin or mutation. Defined as an international biosafety incident, COVID-19 has again encouraged worldwide attention to reconsider the importance of biosafety due to the adverse impact on personal well-being and social stability. Most countries have already taken measures to advocate progress in biosafety-relevant research, aiming to prevent and solve biosafety problems with more advanced techniques and products. Herein, we propose a new concept of biosafety chemistry and reiterate the notion of biosafety materials, which refer to the interdisciplinary integration of biosafety and chemistry or materials. Here, we attempt to illustrate the exquisite association that chemistry and material science possess with biosafety fields and we hope to provide a pragmatic perspective on approaches to utilize the knowledge of these two subjects to handle specific biosafety issues such as detection and disinfection of pathogenic microorganisms, personal and collective protective equipment, vaccine adjuvants and specific drugs, preservation of biogenetic resources for human, animals, and plants. In addition, we hope to convey and promote the idea of multidisciplinary cooperation to strengthen biosafety research and development of relevant products for establishing possibly specific majors to defend national security in the future.

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“…In recent years, fluorescent nanoparticles have been increasingly applied in disease diagnosis and are promising alternative reporters, as their unique chemical and optical properties mean that they have the potential to enhance the sensitivity of lateral flow assays. Many fluorescence nanoparticles were utilized for SARS-CoV-2 detection, including QDs [95], FNDs [96], selenium nanoparticles [65,97], up-converting phosphor particles [98], lanthanide-doped nanoparticles [64], and aggregation-induced emission (AIE) nanoparticles [66].…”

Section: Signal Enhancementmentioning

confidence: 99%

Recent Advances in Novel Lateral Flow Technologies for Detection of COVID-19

Hsiao

Pham

et al. 2021

Biosensors

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The development of reliable and robust diagnostic tests is one of the most efficient methods to limit the spread of coronavirus disease 2019 (COVID-19), which is caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). However, most laboratory diagnostics for COVID-19, such as enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase-polymerase chain reaction (RT-PCR), are expensive, time-consuming, and require highly trained professional operators. On the other hand, the lateral flow immunoassay (LFIA) is a simpler, cheaper device that can be operated by unskilled personnel easily. Unfortunately, the current technique has some limitations, mainly inaccuracy in detection. This review article aims to highlight recent advances in novel lateral flow technologies for detecting SARS-CoV-2 as well as innovative approaches to achieve highly sensitive and specific point-of-care testing. Lastly, we discuss future perspectives on how smartphones and Artificial Intelligence (AI) can be integrated to revolutionize disease detection as well as disease control and surveillance.

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Early Detection of SARS-CoV-2 Seroconversion in Humans with Aggregation-Induced Near-Infrared Emission Nanoparticle-Labeled Lateral Flow Immunoassay

Cited by 140 publications

References 35 publications

Bioconjugated Nanomaterial for Targeted Diagnosis of SARS-CoV-2

Bioconjugated Nanomaterial for Targeted Diagnosis of SARS-CoV-2

Biosafety chemistry and biosafety materials: A new perspective to solve biosafety problems

Recent Advances in Novel Lateral Flow Technologies for Detection of COVID-19

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