Early diagnosis of tuberculous meningitis by an indirect ELISA protocol based on the detection of the antigen ESAT-6 in cerebrospinal fluid

Song, Fu-Xia; Sun, Xuwen; Wang, X.; Nai, Yu; Liu, Z.

doi:10.1007/s11845-013-0980-4

Cited by 23 publications

(18 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Tuberculosis antigen cocktail rapid ICT :60-5 TB antigen cocktail rapid ICT from CSF specimens had a sensitivity of 83.3% and specificity of 68.5%. Song et al 16 reported that the sensitivity TB antigen ESAT-6 from CSF was 88% and the specificity was 92%. In Kashyap et al 17 sensitivity of TB antigen ESAT-6 was 80% and the specificity was 94%.…”

Section: Discussionmentioning

confidence: 99%

The Validity of Mycobacterium Tuberculosis Antigens Cocktail: Esat-6, CFP-10 and Mpt64 in Sputum and Cerebrospinal Fluid for Pulmonary Tuberculosis and Tuberculous Meningitis Diagnosis

Turbawat¹,

Gustiani²,

Noviani³

et al. 2015

IJIHS

View full text Add to dashboard Cite

Objective: To determine the validity of tuberculosis (TB) antigen cocktail (ESAT-6, CFP-10 and MPT64) for pulmonary tuberculosis and TB meningitis diagnosis.Methods: This is a descriptive observational study design. The study was conducted at the Clinical Pathology Laboratory of Dr. Hasan Sadikin General Hospital during September 2012 until March 2013 for the pulmonary tuberculosis study and from January 2014 to May 2014 for the TB meningitis study. The TB antigen cocktail rapid immunochromatography (ICT) test was conduncted on all samples. The sputum and cerebrospinal fluid (CSF) were cultured as gold standards.Results: There were 149 pulmonary and 41 TB meningitis subjects. The sensitivity of rapid ICT TB antigen cocktail for diagnosing pulmonary tuberculosis was 95.7% with a specificity of 87.2%. Of 41 TB meningitis subjects, based on Marais criteria, there were 6 (16%) subjects with a definite TB meningitis, 26 (63%) subjects with probable TB meningitis, and 9 (21%) subjects with possible TB meningitis. The sensitivity and specificity of TB antigen cocktail rapid ICT for TB meningitis diagnosis were 83.3% and 68.5%, respectively. Conclusions:In this study, rapid ICT TB antigen cocktail (ESAT-6, CFP-10 and MPT64) from sputum sample has good validity for diagnosing a pulmonary tuberculosis. Cerebrospinal fluid sample has moderate validity to diagnose TB meningitis.

show abstract

Section: Discussionmentioning

confidence: 99%

The Validity of Mycobacterium Tuberculosis Antigens Cocktail: Esat-6, CFP-10 and Mpt64 in Sputum and Cerebrospinal Fluid for Pulmonary Tuberculosis and Tuberculous Meningitis Diagnosis

Turbawat¹,

Gustiani²,

Noviani³

et al. 2015

IJIHS

View full text Add to dashboard Cite

show abstract

“…Recently several reports revealed potential application of detection of M. tuberculosis −specific antigens in CSF as rapid diagnosis of tuberculosis meningitis 39, 40 . The level of M. tuberculosis Early Secreted Antigenic Target 6 [ESAT-6] was associated with clinical severity, and may be used for the prognosis of tuberculosis meningitis 41, 42 .…”

Section: Conventional Microbiology Methodsmentioning

confidence: 99%

Laboratory Diagnosis of Central Nervous System Infection

Kaplan

Kamboj

et al. 2016

Curr Infect Dis Rep

View full text Add to dashboard Cite

CNS infections are potentially life threatening if not diagnosed and treated early. The initial clinical presentations of many CNS infections are nonspecific making a definitive etiologic diagnosis challenging. Nucleic acid in vitro amplification-based molecular methods are increasingly being applied for routine microbial detection. These methods are a vast improvement over conventional techniques with the advantage of rapid turnaround and higher sensitivity and specificity. Additionally, molecular methods performed on cerebrospinal fluid samples are considered the new gold standard for diagnosis of CNS infection caused by pathogens which are otherwise difficult to detect. Commercial diagnostic platforms offer various monoplex and multiplex PCR assays for convenient testing of targets that cause similar clinical illness. Pan-omic molecular platforms possess potential for use in this area. Although molecular methods are predicted to be widely used in diagnosing and monitoring CNS infections, results generated by these methods need to be carefully interpreted in combination with clinical findings. This review summarizes the currently available armamentarium of molecular assays for diagnosis of central nervous system infections, their application, and future approaches.

show abstract

“…7,8 Hasil pemeriksaan mikroskopik BTA yang baik sangatlah dipengaruhi oleh kualitas bahan pemeriksaan, ketebalan hapusan dan lamanya dekolorisasi, jenis kontras pewarnaan yang digunakan, dan personil laboratorium yang terlatih. Terdapat organisme non-mycobacterial yang dapat memperlihatkan gambaran seperti BTA pada pemeriksaan mikroskopik BTA, yaitu Rhodococcus, Nocardia, Legionella micdadei, kista Cryptosporodium, Isospora, Cyclospora, dan spora Microsporidium.…”

Section: Pendahuluanunclassified

“…Bahan pemeriksaan yang dapat dipakai adalah sputum, serum, urine, dan cairan tubuh lain. [6][7][8] Saat ini terdapat pemeriksaan imunoserologi untuk mendeteksi cocktail antigen TB dengan menggunakan metode rapid immunochromatography (ICT).…”

Section: Pendahuluanunclassified

Gambaran Validitas Pemeriksaan Complex Specific Cocktail Antigen Mycobacterium tuberculosis (ESAT-6, CFP-10, MPT-64) Metode Rapid Immunochromatography pada Bahan Pemeriksaan Sputum dan Serum Penderita Tuberkulosis Paru

Subroto¹,

Parwati²,

Turbawaty³

et al. 2017

mkb

View full text Add to dashboard Cite

AbstrakPenegakan diagnosis tuberkulosis (TB) paru penting dalam mengurangi morbiditas dan mortalitas. Diagnosis laboratorium TB paru berdasar atas pemeriksaan BTA dan kultur M. tuberculosis memiliki sensitivitas rendah. Terdapat pemeriksaan cocktail antigen TB rapid immunochromatography (ICT) yang mendeteksi antigen ESAT-6, CFP-10, Validity of Complex Specific Cocktail Antigen Mycobacterium tuberculosis (ESAT-6, CFP-10, MPT-64) Rapid Immunochromatography Method on Sputum and Serum Samples from Patient with Pulmonary Tuberculosis AbstractEarly diagnosis of pulmonary tuberculosis (TB) is very important in reducing morbidity and mortality. The current diagnosis of TB includes direct staining (acid fast bacilli) or M. tuberculosis culture, but these examinations have a low sensitivity. An assay using rapid ICT cocktail antigen TB is currently available for diagnosing TB. This method can detect ESAT-6, CFP-10, and MPT-64 antigen which is secreted by M. tuberculosis. The aim of this study was to analyze the validity of cocktail antigen TB rapid ICT using sputum and serum with Ogawa culture. This was a crosssectional descriptive observational study. Sputum and serum were collected from patients who were diagnosed as lung TB suspects in the lung and DOTS Clinic of Dr. Hasan Sadikin General Hospital Bandung during the period of July-December 2014 in . Cocktail antigen TB detection assay using two kind of samples (sputum and serum) were evaluated. A total of 68 subjects of33 subjects presented positive culture and 35 presented negative cultures. Positivity rates for sputum and serum were 54.4% and 0%, respectively. Heated sputum assay had a sensitivity of 93.9% and specificity of 82.8%, Serum assay presented a sensitivity of 0% and specificity of100%. Serum were modified by heating at 56oC for 30 minutes. The positivity rate of heated serum was 19.1%. The result of modified serum assay showed a sensitivity of 24.2% and specificity of 85.7%. Conclusion: the sensitivity of the sputum assay is high and the specificity is medium. The sensitivity of this serum assay is low and the specificity is high. [MKB. 2017;49(3):178-85]

show abstract

Early diagnosis of tuberculous meningitis by an indirect ELISA protocol based on the detection of the antigen ESAT-6 in cerebrospinal fluid

Cited by 23 publications

References 15 publications

The Validity of Mycobacterium Tuberculosis Antigens Cocktail: Esat-6, CFP-10 and Mpt64 in Sputum and Cerebrospinal Fluid for Pulmonary Tuberculosis and Tuberculous Meningitis Diagnosis

The Validity of Mycobacterium Tuberculosis Antigens Cocktail: Esat-6, CFP-10 and Mpt64 in Sputum and Cerebrospinal Fluid for Pulmonary Tuberculosis and Tuberculous Meningitis Diagnosis

Laboratory Diagnosis of Central Nervous System Infection

Gambaran Validitas Pemeriksaan Complex Specific Cocktail Antigen Mycobacterium tuberculosis (ESAT-6, CFP-10, MPT-64) Metode Rapid Immunochromatography pada Bahan Pemeriksaan Sputum dan Serum Penderita Tuberkulosis Paru

Contact Info

Product

Resources

About