Early effects of Bdellovibrio infection on the syntheses of protein and RNA of host bacteria

Molecular parasitism in the Escherichia coli-Bdellovibrio bacteriovorus system: translocation of the matrix protein from the host to the parasite outer membrane.

“…Comparing lanes 1 and 3 of Figure 2A with their respective autoradiograms (lanes 1 and 3 of Figure 2B) We infer that the NP found in B. bacteriovorus is an E. coli product also present in uninfected cells. This host protein must be synthesized by the host before infection, since the host's biosynthetic apparatus is shut off after infection (Varon et al, 1969) and furthermore our experiments were carried out in the presence of 100 pjg/ml of streptomycin.…”

“…Maturation begins when the parasite penetrates through the outer membrane into the host periplasm where, -60 min later, synthesis of B. bacteriovorus DNA begins (Matin and Rittenberg, 1972; Rosson and Rittenberg, 1979). The parasite causes shut-off of the host macromolecular synthetic apparatus, and is able to grow on host cells killed by various agents, including antibiotics (Varon et al, 1969;Rittenberg and Shilo, 1970). Despite the fact that the parasite is able to synthetize some precursors (Pritchard et al, 1975), it tends to use the products of degradation of the host's macromolecules (Matin and Rittenberg, 1972;Thomashow and Rittenberg, 1978a, 1978b, 1978cHespell and Odelson, 1978).…”

Combined application of bacterial predation and carbon dioxide aerosols to effectively remove biofilms

“…This apparent discrepancy is accounted for by the character of the predator, B. bacteriovorous HD 100. When this strain attacks a prey bacterium, de novo protein and RNA synthesis within the prey comes to a halt within only few minutes (Varon et al 1969), while those already present are subsequently degraded by the many hydrolytic enzymes produced by B. bacteriovorous HD 100 during the ensuing predation (Rendulic et al 2004). However, a previous study from the authors' group found that fluorescent proteins, such as CFP, are not hydrolyzed and remain stable throughout the full predation episode (Park et al 2011).…”

Protein uptake into E. coll during Bdellovibrio infection

“…Messenger RNA and protein syntheses are inhibited within 3 and 6 min, respectively [32], and the capacity of the host cell to accumulate nutrients in processes driven by the proton motive force is gradually lost thereafter [29,33]. Respiratory capacity is lost more slowly (50% loss in about 30 min), and a gradual increase in non-specific membrane permeability, as measured by the unmasking of /Lgalactosidase activity, has been reported to occur after a lag period [33].…”