Early embryonic requirement for nucleoporin Nup35/NPP-19 in nuclear assembly

Ródenas, Eduardo; Klerkx, Elke P. F.; Ayuso, Cristina de la Cruz; Audhya, Anjon; Askjaer, Peter

doi:10.1016/j.ydbio.2008.12.024

Cited by 44 publications

(47 citation statements)

References 51 publications

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“…In vertebrates, Nup35, also known as Nup53, was previously described to interact with Nup93, Nup155 and Nup205 and is required for NE assembly in vitro [62]. We were able to reveal Nup35 and Nup93 in both the KC and in the fragments of the NE visible in the same preparation, suggesting that some nucleoporins are indeed components of the KC.…”

Section: Discussionsupporting

confidence: 55%

The karyosphere capsule inRana temporariaoocytes contains structural and DNA-binding proteins

Ilicheva

Podgornaya

Bogolyubov

2018

Nucleus

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During the last stages of oogenesis, oocyte chromosomes condense and come close together, forming the so-called karyosphere. Karyosphere formation is accompanied by an essential decrease in transcriptional activity. In the grass frog Rana temporaria, the karyosphere is surrounded by an extrachromosomal capsule that separates the chromosomes from the rest of the nucleoplasm. The karyosphere capsule (KC) of R. temporaria has been investigated in detail at the ultrastructural level, but its protein composition remained largely unknown. We demonstrate here that nuclear actin, especially F-actin, as well as lamins A/C and B are the most abundant proteins of the KC. Key proteins of nuclear pore complexes, such as Nup93 and Nup35, are also detectable in the KC. New antibodies recognizing the telomere-binding protein TRF2 allowed us to localize TRF2 in nuclear speckles. We also found that the R. temporaria KC contains some proteins involved in chromatin remodeling, including topoisomerase II and ATRX. Thus, we believe that KC isolates the chromosomes from the rest of the nucleoplasm during the final period of oocyte growth (late diplotene) and represents a specialized oocyte nuclear compartment to store a variety of factors involved in nuclear metabolism that can be used in future early development.Abbreviations: BrUTP: 5-bromouridine 5’-triphosphate; CytD: cytochalasin D; IGCs: interchromatin granule clasters; IgG: immunoglobulin G; KC: karyosphere capsule; Mw: molecular weight; NE: nuclear envelope; PBS: phosphate buffered saline; SDS-PAGE: sodium dodecyl sulfate polyacrylamide gel electrophoresis; Topo II: topoisomerase II

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Section: Discussionsupporting

confidence: 55%

The karyosphere capsule inRana temporariaoocytes contains structural and DNA-binding proteins

Ilicheva

Podgornaya

Bogolyubov

2018

Nucleus

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“…Development 137 (9) a GFP fusion to NPP-19 as a control (Rodenas et al, 2009). As expected, all GFP::NPP fusions localized to nuclear envelopes ( Fig.…”

Section: Research Articlesupporting

confidence: 62%

TheC. eleganshomolog of nucleoporin Nup98 is required for the integrity and function of germline P granules

Voronina

Seydoux

2010

Development

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All transgenes were driven by the pie-1 promoter (maternal expression), unless otherwise indicated, and were constructed by Gateway cloning (Invitrogen) (Landy, 1989). Coding sequences and predicted 3ЈUTRs were amplified from N2 genomic DNA (npp-7, npp-9, npp-10). Gene structure of npp-10 was analyzed by cDNA sequencing [ends of cDNAs were verified by 5Ј RACE with SL1 primer, and 3Ј RACE with oligo(dT) primer], and the corrected mRNA sequence, including four additional 5Ј exons, was submitted to GenBank (accession number GU174496). The GFP::mCherry construct was generated by PCR fusion. Transgenic lines were generated by microparticle bombardment (Praitis et al., 2001). RNAiRNAi was performed by feeding worms with bacteria expressing doublestranded RNA (Timmons and Fire, 1998 SUMMARYC. elegans P granules are conserved cytoplasmic ribonucleoprotein complexes that are unique to the germline and essential for fertility. During most of germline development, P granules are perinuclear and associate with clusters of nuclear pores. In an RNAi screen against nucleoporins, we have identified a specific nucleoporin essential for P granule integrity and function. The C. elegans homolog of vertebrate Nup98 (CeNup98) is enriched in P granules and associates with the translationally repressed, P granuleenriched mRNA nos-2 (nanos homolog). Loss of CeNup98 causes P granules to disperse in the cytoplasm and to release nos-2 mRNA. Embryos depleted for CeNup98 express a nos-2 3ЈUTR reporter prematurely. In the mouse, Nup98 immunoprecipitates with the germ granule component MVH. Our findings suggest that, in germ cells, the function of Nup98 extends beyond transport at the nuclear pore to include mRNA regulation in the cytoplasm.

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“…Knockdown of several npp genes, including FG repeat-containing npp-1, npp-4, npp-11, as well as npp-2, npp-3, and npp-13 causes a reduction in nuclear size, which typically reflects diminished nuclear import (Galy et al 2003). More direct evidence for a role in nuclear protein import has been established for NPP-19/NUP35 because nuclear levels of PCN-1/PCNA and PIE-1 are strongly reduced in npp-19(RNAi) embryos (Rodenas et al 2009). Presumably, additional NPPs are involved in nucleocytoplasmic transport, but due to the pronounced block in postmitotic NE assembly further experiments are required to address this (Galy et al 2003(Galy et al , 2006Franz et al 2005;Fernandez and Piano 2006).…”

Section: Npps and Npc Functionmentioning

confidence: 99%

Cell Biology of theCaenorhabditis elegansNucleus

Cohen-Fix

Askjaer

2017

Genetics

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Studies on the Caenorhabditis elegans nucleus have provided fascinating insight to the organization and activities of eukaryotic cells. Being the organelle that holds the genetic blueprint of the cell, the nucleus is critical for basically every aspect of cell biology. The stereotypical development of C. elegans from a one cell-stage embryo to a fertile hermaphrodite with 959 somatic nuclei has allowed the identification of mutants with specific alterations in gene expression programs, nuclear morphology, or nuclear positioning. Moreover, the early C. elegans embryo is an excellent model to dissect the mitotic processes of nuclear disassembly and reformation with high spatiotemporal resolution. We review here several features of the C. elegans nucleus, including its composition, structure, and dynamics. We also discuss the spatial organization of chromatin and regulation of gene expression and how this depends on tight control of nucleocytoplasmic transport. Finally, the extensive connections of the nucleus with the cytoskeleton and their implications during development are described. Most processes of the C. elegans nucleus are evolutionarily conserved, highlighting the relevance of this powerful and versatile model organism to human biology.KEYWORDS Caenorhabditis elegans; chromatin; gene expression; lamin; LEM-domain proteins; LINC; P granule; nuclear pore complex; nuclear envelope; nucleocytoplasmic transport; nucleolus; WormBook TABLE OF CONTENTSAbstract 25 C. elegans as a model system to study cell biology of the nucleus 26 Structural components of the nucleus 27

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Early embryonic requirement for nucleoporin Nup35/NPP-19 in nuclear assembly

Cited by 44 publications

References 51 publications

The karyosphere capsule inRana temporariaoocytes contains structural and DNA-binding proteins

The karyosphere capsule inRana temporariaoocytes contains structural and DNA-binding proteins

TheC. eleganshomolog of nucleoporin Nup98 is required for the integrity and function of germline P granules

Cell Biology of theCaenorhabditis elegansNucleus

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