Early Expression of Carbonic Anhydrase II (CAII) in Transitory Glial Cells of the Developing Murine Nervous System

Benjelloun, Soumaya; Delaunoy, J. P.; Gomès, Danielle; Vitry, F. De; Langui, Dominique; Dupouey, P.

doi:10.1159/000112250

Cited by 11 publications

(9 citation statements)

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“…A certain number of astrocytes may arise from the generative matrix without passing through a previous phase as radial glia (LeVine and Goldman, 1988). Intermediate forms seen in the process of transformation have been described in some rodent species (Benjelloun at al., 1986;Levine and Goldman, 1988). Some of these forms transitorily express carbon anhydrase 11, an oligodendrocyte marker (Benjelloun et al, 1986).…”

Section: Discussionmentioning

confidence: 99%

“…Some of these forms transitorily express carbon anhydrase 11, an oligodendrocyte marker (Benjelloun et al, 1986). In this connection, it has been shown that the rat radial glia are a precursor of both astrocytes and oligodendrocytes (Pixley and De Vellis, 1984;Benjelloun et al, 1986;Hirano and Goldman, 1988). The transformation of radial glia into astrocytes may be influenced by neurons that trigger the expression of glial glutamine synthetase, an astrocyte marker (Linser and Perkins, 1987).…”

Section: Discussionmentioning

confidence: 99%

“…Intermediate forms seen in the process of transformation have been described in some rodent species (Benjelloun at al., 1986;Levine and Goldman, 1988). Some of these forms transitorily express carbon anhydrase 11, an oligodendrocyte marker (Benjelloun et al, 1986). In this connection, it has been shown that the rat radial glia are a precursor of both astrocytes and oligodendrocytes (Pixley and De Vellis, 1984;Benjelloun et al, 1986;Hirano and Goldman, 1988).…”

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confidence: 99%

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Postnatal development of vimentin‐positive cells in the rabbit superior colliculus

Pulido‐Caballero

Jiménez‐Sampedro

Echevarría‐Aza

et al. 1994

J of Comparative Neurology

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The present study examined the postnatal development of the radial glia in the rabbit superior colliculus during the first 40 postnatal days. An antivimentin monoclonal antibody and the carbocyanine fluorescent tracer DiI were used in order to investigate the development of laminar connectivity in the superior colliculus. We focused our study on the superficial gray layer, the intermediate layer, and the deep layers of the superior colliculus, the periaqueductal gray matter (PAGM), and the medial intercollicular region. Vimentin-positive structures of glial lineage consisted of 1) the main radial system, which in the newborn rabbit was made up of wavy fibers that ran from the aqueduct to the pial surface, where they terminated in end-feet. At postnatal day 15, these fibers diminished to 100-200 microns long wavy tracts, which emanated from the aqueduct, and to a few straight or arched fragments in the superficial gray layer; 2) the median ventricular formation, which extends from mesencephalic aqueduct to the intercollicular sulcus, was characterized by a series of ascending, vimentin-positive fibers, some of large caliber, which persisted until postnatal day 40; 3) the tangential fiber system, which was made up of fibers that diverged from the median ventricular formation and of a number of short tracts running perpendicular to the periaqueductal radial fibers; these structures may provide support for migrating subpopulations of neurons; 4) immature and mature-like protoplasmic and fibrous astrocytes, which appeared during the second postnatal week. Thereafter, the number of vimentin-positive astrocytes decreased sharply. Our findings generally support earlier descriptions of the radial glia, except for the persistence, in superficial layers of the superior colliculus, of straight and curved fragments of fibers, which may participate in the organization of visual afferents at this level.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Postnatal development of vimentin‐positive cells in the rabbit superior colliculus

Pulido‐Caballero

Jiménez‐Sampedro

Echevarría‐Aza

et al. 1994

J of Comparative Neurology

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“…These proteins have been detected at ED 9 in the mouse brain (Cochard and Paulin, 1984;Foster et al, 1987). The CA I1 protein is widely considered to be a specific oligodendroglial marker; this antigent first appears at ED 16 in the embryonic mouse CNS (Benjelloun et al, 1986). The other markers of oligodendroglial precursor cells (A2B5 and galactocerebroside) appear later (Abney et al, 1981).…”

Section: Discussionmentioning

confidence: 99%

“…Within 18-24 hr of plating, the mRNA+ cells from E-12 hypothalami are very small and have a characteristically high nucleocytoplasmic ratio, as do embryonic cells. The mRNA+ protein+ cells, which appear 4-5 days later, present a bipolar or monopolar aspect, with a very long process at one pole and often a short and thin process at the other pole; they are reminiscent of the CA I1 protein transitory glial cells in the early developing mouse brain (Benjelloun et al, 1986). At the postnatal stage, mRNA+ protein+ cells present the characteristic morphology of the adult oligodendrocyte: a spherical or polygonal cell body with an excentric nucleus (Peters et al, 1976) and occasionally a few delicate processes.…”

Section: Discussionmentioning

confidence: 99%

Expression of carbonic anhydrase II gene in early brain cells as revealed by in situ hybridization and immunohistochemistry

Vitry

Gomès

Rataboul

et al. 1989

J of Neuroscience Research

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A mouse carbonic anhydrase (CA II) complementary(c) DNA probe was used for in situ hybridization on mouse brain cultured cells in order to follow CA II gene expression during brain development. An improved method was established using biotinated probes that resulted in a high sensitivity and an absence of background; this method could be combined with immunohistochemistry. Hypothalamic cells of embryonic day (ED) 12-14 mice were cultured for various periods. Chronologic appearance of CA II messenger(m)RNA and protein was studied. The CA II gene transcripts are detectable as early as ED 12-13, although the protein they encode is not detectable until ED 17-18. Gene expression is restricted to 0.1% of the total population. Northern blot analysis confirmed the presence of CA II transcripts in embryonic hypothalamus. At postnatal stage, the majority of glial cells express both the CA II mRNA and the protein. Our results favour the early appearance of a glial lineage in a precise area of the developing CNS. The precocity of CA II gene transcription makes in situ hybridization an invaluable approach in defining the onset of nerve cell lineages during embryonic development.

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Prenatal gliogenesis in the developing cerebrum of the mouse

Choi

1988

Glia

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A correlative light microscopic, ultrastructural, and immunocytochemical study was made of the brains of C57BL/6J mice obtained between embryonic day (E-) 11 to postnatal day (P-) 3. The deployment of radially oriented glial cells within the neopallium was already apparent by E-12, at which time the expanded endfeet of processes abutting the basement membrane at the pial surface showed ultrastructural evidence of glial differentiation. Scattered, horizontally arranged glial cells were also observed within the marginal zone prior to the arrival of the cortical plate neurons. Indirect immunofluorescence for glial fibrillary acidic protein (GFAP) was detected at the outer margins of the neopallium at E-12, antedating the expression of GFAP in radially organized fibers. Radial fibers traversing the full thickness of the neopallium became strongly immunoreactive for GFAP only at and after day E-16. These findings provide evidence of early gliogenesis in the developing mouse forebrain and indicate that GFAP is as readily demonstrable in mouse astrocytes and radial glial fibers as it is in those of man and subhuman primates.

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Early Expression of Carbonic Anhydrase II (CAII) in Transitory Glial Cells of the Developing Murine Nervous System

Cited by 11 publications

References 11 publications

Postnatal development of vimentin‐positive cells in the rabbit superior colliculus

Postnatal development of vimentin‐positive cells in the rabbit superior colliculus

Expression of carbonic anhydrase II gene in early brain cells as revealed by in situ hybridization and immunohistochemistry

Prenatal gliogenesis in the developing cerebrum of the mouse

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