Early growth response 1 (EGR-1) is a transcriptional regulator of mitochondrial carrier homolog 1 (MTCH 1)/presenilin 1-associated protein (PSAP)

Nelo-Bazán, María Alejandra; Latorre, Pedro; Bolado-Carrancio, Alfonso; Pérez-Campo, Flor M.; Echenique-Robba, Pablo; Rodríguez‐Rey, José C.; Carrodeguas, José A.

doi:10.1016/j.gene.2015.12.014

Cited by 8 publications

(7 citation statements)

References 57 publications

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“…One bait, MTCH1, exhibited increased proximity interaction with ATAD3A as compared to the other baits in the dataset. MTCH1 is an outer membrane carrier protein of unknown function ( Nelo-Bazán et al, 2016 ) which may interact with the outer membrane segment of ATAD3 in a more stable manner.…”

Section: Resultsmentioning

confidence: 99%

ATAD3A has a scaffolding role regulating mitochondria inner membrane structure and protein assembly

et al. 2021

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Section: Resultsmentioning

confidence: 99%

ATAD3A has a scaffolding role regulating mitochondria inner membrane structure and protein assembly

et al. 2021

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“…Moreover, we found several proteins involved in the apoptotic pathway, as the transcription factor Stwl 23 , the mitochondrial carrier homolog 1 (Mtch) 24 , ALG-2 interacting protein X 21 and the GTPase Rab1 25 , 26 . Among these proteins, we focused our attention on the ALG-2 interacting protein X, also called Alix.…”

Section: Resultsmentioning

confidence: 99%

The multifunctional polydnavirus TnBVANK1 protein: impact on host apoptotic pathway

Salvia

Grossi

Amoresano

et al. 2017

Sci Rep

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Toxoneuron nigriceps (Hymenoptera, Braconidae) is an endophagous parasitoid of the larval stages of the tobacco budworm, Heliothis virescens (Lepidoptera, Noctuidae). The bracovirus associated with this wasp (TnBV) is currently being studied. Several genes expressed in parasitised host larvae have been isolated and their possible roles partly elucidated. TnBVank1 encodes an ankyrin motif protein similar to insect and mammalian IκB, an inhibitor of the transcription nuclear factor κB (NF-κB). Here we show that, when TnBVank1 was stably expressed in polyclonal Drosophila S2 cells, apoptosis is induced. Furthermore, we observed the same effects in haemocytes of H. virescens larvae, after TnBVank1 in vivo transient transfection, and in haemocytes of parasitised larvae. Coimmunoprecipitation experiments showed that TnBVANK1 binds to ALG-2 interacting protein X (Alix/AIP1), an interactor of apoptosis-linked gene protein 2 (ALG-2). Using double-immunofluorescence labeling, we observed the potential colocalization of TnBVANK1 and Alix proteins in the cytoplasm of polyclonal S2 cells. When Alix was silenced by RNA interference, TnBVANK1 was no longer able to cause apoptosis in both S2 cells and H. virescens haemocytes. Collectively, these results indicate that TnBVANK1 induces apoptosis by interacting with Alix, suggesting a role of TnBVANK1 in the suppression of host immune response observed after parasitisation by T. nigriceps.

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“…Moreover, this mitochondrial-dependent protein translation could be further enhanced by overexpression of EGR1 and ATF2 in cells (Fig. 3D), and both proteins have been shown to be related with the function of mitochondria (25,26). To prove further that mitochondrial localization was necessary for effective viral protein translation, two reported mitochondrial RNA targeting signals (Rnase P binding motif, RP and MRP binding motif, MRP) were inserted upstream of the SARS-CoV-2 S, E or ORF8 genes (Fig.3E).…”

Section: Main Textmentioning

confidence: 89%

A novel viral protein translation mechanism reveals mitochondria as a target for antiviral drug development

Cheng

Chen

et al. 2020

Preprint

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The ongoing Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) pandemic has acutely highlighted the need to identify new treatment strategies for viral infections. Here we present a pivotal molecular mechanism of viral protein translation that relies on the mitochondrial translation machinery. We found that rare codons such as Leu-TTA are highly enriched in many viruses, including SARS-CoV-2, and these codons are essential for the regulation of viral protein expression. SARS-CoV-2 controls the translation of its spike gene by hijacking host mitochondria through 5' leader and 3'UTR sequences that contain mitochondrial localization signals and activate the EGR1 pathway. Mitochondrial-targeted drugs such as lonidamine and polydatin significantly repress rare codon-driven gene expression and viral replication. This study identifies an unreported viral protein translation mechanism and opens up a novel avenue for developing antiviral drugs.

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Early growth response 1 (EGR-1) is a transcriptional regulator of mitochondrial carrier homolog 1 (MTCH 1)/presenilin 1-associated protein (PSAP)

Cited by 8 publications

References 57 publications

ATAD3A has a scaffolding role regulating mitochondria inner membrane structure and protein assembly

ATAD3A has a scaffolding role regulating mitochondria inner membrane structure and protein assembly

The multifunctional polydnavirus TnBVANK1 protein: impact on host apoptotic pathway

A novel viral protein translation mechanism reveals mitochondria as a target for antiviral drug development

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