Early host–microbe interaction in a peri‐implant oral mucosa‐biofilm model

Mikolai, Carina; Kommerein, Nadine; Ingendoh-Tsakmakidis, Alexandra; Winkel, Andreas; Falk, Christine S.; Stiesch, Meike

doi:10.1111/cmi.13209

Cited by 18 publications

(15 citation statements)

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“…For all experiments, a well-established multispecies biofilm model was used [22,23,51,52]. This model was chosen because the distribution of the integrated bacterial species, S. oralis, A. naeslundii, V. dispar and P. gingivalis, reflects the situation in early oral biofilms and it has proven to be highly reproducible.…”

Section: Discussionmentioning

confidence: 99%

Plant-based oral care product exhibits antibacterial effects on different stages of oral multispecies biofilm development in vitro

et al. 2021

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Background Excessive biofilm formation on surfaces in the oral cavity is amongst the main reasons for severe infection development like periodontitis and peri-implantitis. Mechanical biofilm removal as well as the use of adjuvant antiseptics supports the prevention of pathogenic biofilm formation. Recently, the antibacterial effect of the oral care product REPHA-OS®, based on medicinal plant extracts and essential oils, has been demonstrated on oral pathogens grown on agar plates. In the present study, the effectiveness of the product on medical relevant oral biofilm development should be demonstrated for the first time. Methods An established in vitro oral multispecies biofilm, composed of Streptococcus oralis, Actinomyces naeslundii, Veillonella dispar and Porphyromonas gingivalis, was used to analyze the antibacterial effect of different REPHA-OS® concentrations on planktonic bacteria, biofilm formation and mature biofilms. It was quantified using metabolic activity assays and live/dead fluorescence staining combined with three-dimensional confocal laser-scanning microscopy. Additionally, effects on species distribution inside the biofilm were assessed by means of quantitative real-time PCR. Results REPHA-OS® showed statistically significant antimicrobial effects on all stages of biofilm development: a minimal inhibitory concentration of 5% could be detected for both, for planktonic bacteria and for biofilm formation. Interestingly, only a slightly higher concentration of 10% was necessary to completely kill all bacteria in mature biofilms also. In contrast, an influence on the biofilm matrix or the species distribution could not be observed. The effect could be attributed to the herbal ingredients, not to the contained ethanol. Conclusion The strong antibacterial effect of REPHA-OS® on different stages of oral biofilm development strengthens its application as an alternative adjuvant in oral care therapies.

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Section: Discussionmentioning

confidence: 99%

Plant-based oral care product exhibits antibacterial effects on different stages of oral multispecies biofilm development in vitro

et al. 2021

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“…Because peri-implantitis has become a topic of interest, several approaches were presented to mimic the bacterial colonization of surfaces [23,24]. Recent studies have used mixed microbial infection by oral lavage to facilitate bacterial adherence to the implant surface and to induce an inflammatory host response in animal models [10].…”

Section: Discussionmentioning

confidence: 99%

Introducing a Novel Experimental Model for Osseo-Disintegration of Titanium Dental Implants Induced by Monobacterial Contamination: An In-Vivo Feasibility Study

et al. 2021

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Background and Objectives: The aim of the current study was to establish an osseo-disintegration model initiated with a single microorganism in mini-pigs. Materials and Methods: A total of 36 titanium dental implants (3.5 mm in diameter, 9.5 mm in length) was inserted into frontal bone (n: 12) and the basis of the corpus mandible (n: 24). Eighteen implants were contaminated via inoculation of Enterococcus faecalis. Six weeks after implant insertion, bone-to-implant contact (BIC) ratio, interthread bone density (ITBD), and peri-implant bone density (PIBD) were examined. In addition to that, new bone formation was assessed via fluorescence microscopy, histomorphometry, and light microscopical examinations. Results: Compared to the sterile implants, the contaminated implants showed significantly reduced BIC (p < 0.001), ITBD (p < 0.001), and PBD (p < 0.001) values. Around the sterile implants, the green and red fluorophores were overlapping and surrounding the implant without gaps, indicating healthy bone growth on the implant surface, whereas contaminated implants were surrounded by connective tissue. Conclusions: The current experimental model could be a feasible option to realize a significant alteration of dental-implant osseointegration and examine novel surface decontamination techniques without impairing local and systemic inflammatory complications.

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“…The Stiesch group presented a study model for host-microbe interaction on a related topic of inflamed mucosa in the periimplant diseases (Ingendoh-Tsakmakidis et al, 2019;Mikolai et al, 2020). Briefly, they developed a novel, simulating in-vitro three-dimensional model comprised of three componentsorganotypic oral mucosa, implant material, and oral biofilmwith structural assembly close to the native situation to investigate the early host-microbe interaction (Ingendoh-Tsakmakidis et al, 2019).…”

Section: Host-microbiome Interactionmentioning

confidence: 99%

“…Disruption of homeostasis was accompanied by an enhanced immune response with upregulation of inflammatory-related genes and increased cytokine secretion. This model of hostmicrobe interaction at the peri-implant site may pinpoint changes associated with disease onset and provide the basis to improve strategies for prevention (Ingendoh-Tsakmakidis et al, 2019;Mikolai et al, 2020).…”

Section: Host-microbiome Interactionmentioning

confidence: 99%

Current State and Challenges of the Global Outcomes of Dental Caries Research in the Meta-Omics Era

Moussa

Ahmad

Mansour

et al. 2022

Front. Cell. Infect. Microbiol.

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Despite significant healthcare advances in the 21st century, the exact etiology of dental caries remains unsolved. The past two decades have witnessed a tremendous growth in our understanding of dental caries amid the advent of revolutionary omics technologies. Accordingly, a consensus has been reached that dental caries is a community-scale metabolic disorder, and its etiology is beyond a single causative organism. This conclusion was based on a variety of microbiome studies following the flow of information along the central dogma of biology from genomic data to the end products of metabolism. These studies were facilitated by the unprecedented growth of the next- generation sequencing tools and omics techniques, such as metagenomics and metatranscriptomics, to estimate the community composition of oral microbiome and its functional potential. Furthermore, the rapidly evolving proteomics and metabolomics platforms, including nuclear magnetic resonance spectroscopy and/or mass spectrometry coupled with chromatography, have enabled precise quantification of the translational outcomes. Although the majority supports ‘conserved functional changes’ as indicators of dysbiosis, it remains unclear how caries dynamics impact the microbiota functions and vice versa, over the course of disease onset and progression. What compounds the situation is the host-microbiota crosstalk. Genome-wide association studies have been undertaken to elucidate the interaction of host genetic variation with the microbiome. However, these studies are challenged by the complex interaction of host genetics and environmental factors. All these complementary approaches need to be orchestrated to capture the key players in this multifactorial disease. Herein, we critically review the milestones in caries research focusing on the state-of-art singular and integrative omics studies, supplemented with a bibliographic network analysis to address the oral microbiome, the host factors, and their interactions. Additionally, we highlight gaps in the dental literature and shed light on critical future research questions and study designs that could unravel the complexities of dental caries, the most globally widespread disease.

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Early host–microbe interaction in a peri‐implant oral mucosa‐biofilm model

Cited by 18 publications

References 40 publications

Plant-based oral care product exhibits antibacterial effects on different stages of oral multispecies biofilm development in vitro

Plant-based oral care product exhibits antibacterial effects on different stages of oral multispecies biofilm development in vitro

Introducing a Novel Experimental Model for Osseo-Disintegration of Titanium Dental Implants Induced by Monobacterial Contamination: An In-Vivo Feasibility Study

Current State and Challenges of the Global Outcomes of Dental Caries Research in the Meta-Omics Era

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