The work is devoted to review the various methods and approaches currently used within the framework of the concept of optical «biopsy» in the study of blood perfusion and metabolism of human organs tissues. The purpose of the review was to study the features and areas of application of these methods and to assess further application possibilities for intraoperative data acquisition in minimally invasive surgery of hepatopancreatoduodenal organs. Preliminary experimental studies were performed with fluorescence spectroscopy method in pathologies of the common bile duct, gallbladder and liver. The data obtained in the work show the prospects for this method application, and serve as the basis for further research in order to obtain additional diagnostic criteria.