Early immunodiagnosis of fasciolosis in ruminants using recombinant Fasciola hepatica cathepsin L-like protease

Cornelissen, J.B.W.J.; Gaasenbeek, C.P.H.; Borgsteede, F.H.M.; Holland, Wicher; Harmsen, Michiel M.; Boersma, W.J.A.

doi:10.1016/s0020-7519(01)00175-8

Cited by 117 publications

(84 citation statements)

References 22 publications

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“…ELISA was selected in the current study for evaluation of the diagnostic potency of the isolated glycoproteins based on its high sensitivity and possibility of processing many serum samples simultaneously (Cornelissen et al 2001;Charlier et al 2008). In the present study, it was concluded that FII glycoprotein could detect experimental F. gigantica infection in rabbits as early as one week post infection with high significance than FI and crude extract.…”

Section: Serine (Ser)mentioning

confidence: 68%

The role of Ser-(Arg-Ser-Arg-Ser-GlucNAc)19-GlucNAc Fasciola gigantica glycoprotein in the diagnosis of prepatent fasciolosis in rabbits

Abdel-Rahman¹,

Mohamed

Abdel‐Rahman

et al. 2014

J Parasit Dis

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In the present study, the carbohydrate structures associated with Fasciola gigantica adult worm were identified by indirect hemagglutination inhibition test. Glucose was found to be the main monosaccharide associated with the fluke. According to indirect hemagglutination inhibition results, purification of glycoprotein fractions from worm crude extract was carried out by affinity chromatography immobilized glucose agarose gel and Con-A lectin columns. The isolated glycoprotein fractions, FI and FII, were characterized by SDS-PAGE which revealed one band in FI of 26 kDa and another one band of 19.5 kDa in FII compared with 12 bands associated with whole worm extract. Both fractions were also characterized by isoelectric focusing technique which proved that both bands were acidic in nature with pIs 6.4 and 6.5 respectively. The comparative diagnostic evaluation of the two isolated glycoprotein fractions and crude extract of experimental fasciolosis in rabbits by ELISA revealed that FII was more potent in the diagnosis during prepatent (first week post infection) and patent periods (10 weeks post infection) than FI and crude extract. Moreover, infected rabbit sera at ten weeks post infection identified both bands; 26 and 19.5 kDa in western blot analysis confirming its immunodiagnostic activities which was proved previously by ELISA. FII proved potency in diagnosis of fasciolosis in 200 buffalo serum samples of different ages and sexes using ELISA which recorded 95 % positive and 5 % negative samples. Moreover, the detailed structural analyses of the most potent fraction, F11, using mass spectrum was made and elucidated chemical structure; O-glycan [Ser-(Arg-Ser-Arg-Ser-GlucNAc) 19 -GlucNAc]. The present result introduces GlucNAc rich fraction of F .gigantica that can be used successfully in the diagnosis of acute and chronic fasciolosis.

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Section: Serine (Ser)mentioning

confidence: 68%

The role of Ser-(Arg-Ser-Arg-Ser-GlucNAc)19-GlucNAc Fasciola gigantica glycoprotein in the diagnosis of prepatent fasciolosis in rabbits

Abdel-Rahman¹,

Mohamed

Abdel‐Rahman

et al. 2014

J Parasit Dis

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“…Die Prävalenzunterschiede basieren dabei unter anderem auf der unterschiedlichen Sensitivität der Methoden: Die Untersuchung der Galle ist wesentlich empfindlicher als die makroskopische Untersuchung der Leber (Braun et al, 1995 (Hauser, 1977). In derselben Arbeit wurde jedoch gezeigt, dass die Koproskopie lediglich 56 % der Tiere erfasst, die mit der Gallenuntersuchung als positiv diagnostiziert wurden.Weitere Untersuchungsmethoden sind der serologische Nachweis von Antikörpern (Cornelissen et al, 2001;Reichel, 2002) oder Antigen (Leclipteux et al, 1998) sowie der Nachweis von Koproantigen (Mezo et al, 2004 (Rapsch et al, 2006).…”

Section: Prävalenz Im Rindunclassified

Bovine Fasciolose in der Schweiz: Bedeutung und Bekämpfung

Knubben‐Schweizer¹,

Deplazes²,

Torgerson³

et al. 2010

Schweizer Archiv für Tierheilkunde

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“…For this reason, they are valuable in diagnosis (8). Purified and recombinant antigens have begun to be used in recent years (11)(12)(13).…”

Section: Introductionmentioning

confidence: 99%

“…The adult stage of F. hepatica secretes several cysteine proteases and the cathepsin L-like proteinases are proposed to be involved in activities such as invasion of tissues, feeding, immune evasion, or egg shell formation (14). Cathepsin L1 and L2 proteins are potential molecules to be used in diagnostic methods (8,11,12) and in immunization studies for protection purpose (15)(16)(17)(18)(19) as they are specific to F. hepatica.…”

Section: Introductionmentioning

confidence: 99%

Regional Differences In The Amino Acid Sequence Of Fasciola Hepatica Cathepsin L2 From Turkey Isolate

Başpinar¹,

Çelik²,

Balik³

et al. 2010

TUTFD

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Objective: This study describes cloning and sequence analysis of the gene coding for the cathepsin L2 gene from a F. hepatica isolate from Turkey. Methods:The adult form of F. hepatica was collected from the infected cattle liver and cDNA was obtained following total RNA isolation. After amplification of the gene by PCR, ligation and transformation was performed. Finally, DNA sequencing and phylogenetic analysis were performed.Results: The gene consists of an open reading frame of 981 nucleotides corresponding for 327 amino acids. There are five amino acid positions where Firat F. hepatica cathepsin L2 differs from the F. hepatica cathepsin L2 Dublin strain. This sequence was the first cathepsin L2 gene from a Turkey isolate and the second cathepsin L2 sequence entered into the database. A comparative amino acid sequence analysis indicated that there is about 99% homology with the F. hepatica cathepsin L2 reported in the literature, 98% homology with the F. hepatica cathepsin L-like protease, and 85% homology with the F. hepatica cathepsin L1 isolated in the Elazığ region at the nucleotide level. Conclusion:The amino acid differences between sequences could be evaluated for further immunization and diagnosis studies.

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Early immunodiagnosis of fasciolosis in ruminants using recombinant Fasciola hepatica cathepsin L-like protease

Cited by 117 publications

References 22 publications

The role of Ser-(Arg-Ser-Arg-Ser-GlucNAc)19-GlucNAc Fasciola gigantica glycoprotein in the diagnosis of prepatent fasciolosis in rabbits

The role of Ser-(Arg-Ser-Arg-Ser-GlucNAc)19-GlucNAc Fasciola gigantica glycoprotein in the diagnosis of prepatent fasciolosis in rabbits

Bovine Fasciolose in der Schweiz: Bedeutung und Bekämpfung

Regional Differences In The Amino Acid Sequence Of Fasciola Hepatica Cathepsin L2 From Turkey Isolate

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