Early-life exposure to bisphenol A induces dysregulation of lipid homeostasis by the upregulation of SCD1 in male mice

Fang, Ruyue; Yang, Shaohua; Gu, Xiaozhen; Li, Changqing; Bi, Nanxi; Wang, Huili

doi:10.1016/j.envpol.2022.119201

Cited by 19 publications

(13 citation statements)

References 42 publications

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“…Furthermore, it has been proposed that BPS and BPF enhance lipid accumulation and promote the differentiation of preadipocytes into adipocytes through the PPARγ signaling pathway [ 38 – 40 ]. Additionally, the exposure to BPA and BPS resulted in the differential expression of genes related to lipid metabolism and adipogenesis and also increasing 3T3-L1 adipocyte differentiation [ 41 – 43 ]. Notably, BPA can disturb glucose homeostasis and influence the pancreatic β-cell function through multiple mechanisms [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

Serum glycolipids mediate the relationship of urinary bisphenols with NAFLD: analysis of a population-based, cross-sectional study

Jia

2023

Environ Health

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Background Bisphenol A (BPA) and its substitutes bisphenol S (BPS) and bisphenol F (BPF) are endocrine-disrupting chemicals widely used in consumer products, which have been proposed to induce various human diseases. In western countries, one of the most common liver diseases is non-alcoholic fatty liver disease (NAFLD). However, studies on the associations of the three bisphenols with NAFLD in human beings are scarce. Methods We included 960 participants aged ≥ 20 years from the NHANES 2013–16 who had available data on levels of urinary BPA, BPS and BPF. The hepatic steatosis index (HSI) > 36 was used to predict NAFLD. Logistic regression analysis and mediation effect analysis were used to evaluate the associations among bisphenols, glycolipid-related markers and NAFLD. Results A total of 540 individuals (56.3%) were diagnosed with NAFLD, who had higher concentrations of BPA and BPS but not BPF than those without NAFLD. An increasing trend in NAFLD risks and HSI levels was observed among BPA and BPS tertiles (p for trend < 0.05). After adjustment for confounders, elevated levels of BPA or BPS but not BPF were significantly associated with NAFLD. The odds ratio for NAFLD was 1.581 (95% confidence intervals [CI]: 1.1–2.274, p = 0.013) comparing the highest with the lowest tertile of BPA and 1.799 (95%CI: 1.2462.597, p = 0.002) for BPS. Mediation effect analysis indicated that serum high-density lipoprotein cholesterol and glucose had a mediating effect on the relationships between bisphenols and NAFLD. Conclusions The present study showed that high exposure levels of BPA and BPS increased NAFLD incidence, which might be mediated through regulating glycolipids metabolism. Further studies on the role of bisphenols in NAFLD are warranted.

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Section: Discussionmentioning

confidence: 99%

Serum glycolipids mediate the relationship of urinary bisphenols with NAFLD: analysis of a population-based, cross-sectional study

Jia

2023

Environ Health

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“…Scd1 was a downstream gene of PPAR‐ γ , a key enzyme for de novo fat synthesis, and cis ‐TSG significantly downregulated Scd1 , thereby reducing the synthesis and accumulation of TG in the liver and increasing the accumulation of fatty acids [48]. The increase in free fatty acids can further lead to inflammation.…”

Section: Discussionmentioning

confidence: 99%

Comparison of metabolic differences between two geometrical isomers based on multi‐omics strategy

Ge,

Gao,

Zhang

et al. 2023

Separation Science Plus

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Abstract2,3,5,4′‐tetrahydroxy stilbene‐2‐O‐β‐D‐glucoside is the main compound of Polygonum multiflorum. There are two geometrical isomers of 2,3,5,4′‐tetrahydroxy stilbene‐2‐O‐β‐D‐glucoside. Several studies have shown that cis‐2,3,5,4′‐tetrahydroxy stilbene‐2‐O‐β‐D‐glucoside may be the risk compound of hepatotoxicity in Polygonum multiflorum Thunb. and there are few comprehensive comparisons on the different impacts of cis‐2,3,5,4′‐tetrahydroxy stilbene‐2‐O‐β‐D‐glucoside and trans‐2,3,5,4′‐tetrahydroxy stilbene‐2‐O‐β‐D‐glucoside about metabolic characteristics. This paper aimed to provide a comprehensive assessment of the metabolomics and lipidomics studies of the differences in metabolic characteristics of cis‐2,3,5,4′‐tetrahydroxy stilbene‐2‐O‐β‐D‐glucoside and trans‐2,3,5,4′‐tetrahydroxy stilbene‐2‐O‐β‐D‐glucoside. The results showed that trans‐2,3,5,4′‐tetrahydroxy stilbene‐2‐O‐β‐D‐glucoside and cis‐2,3,5,4′‐tetrahydroxy stilbene‐2‐O‐β‐D‐glucoside significantly affected 47 metabolites. The main interfering pathways included alpha‐linolenic acid, linoleic acid (LA) metabolism, arachidonic acid (AA) metabolism, and fatty acid metabolism. According to the transcriptomics study, the altered genes were mainly enriched in LA metabolism, AA metabolism, and fatty acid metabolism. Two genes (Runx3 and Scd1) were mainly involved in the cell cycle and fatty acid metabolism in the cis‐2,3,5,4′‐tetrahydroxy stilbene‐2‐O‐β‐D‐glucoside group, and one enzyme (Phospholipase B1) was related to alpha‐linolenic acid, LA metabolism and AA metabolism in the trans‐2,3,5,4′‐tetrahydroxy stilbene‐2‐O‐β‐D‐glucoside group. These would provide useful information for further investigation on therapeutic and hepatotoxic mechanisms of Polygonum multiflorum Thunb.

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“…A bicinchoninic acid (BCA) protein assay kit (Beyotime, Shanghai, China) was used to determine protein concentrations and adjust them to the same concentration. The expression of proteins was assayed according to our previously described method (Fang et al ., 2022). After washing in Tris‐Buffered Saline with Tween‐20 (TBST) for several times, membranes were incubated by secondary antibody (Boster Biological Technology, Pleasanton, CA, USA) with 1:2000 dilutions at 37 °C for 1 h. Primary antibodies used were as follows, mouse anti‐β‐actin (Santa Cruz Biotechnology, Cat.…”

Section: Methodsmentioning

confidence: 99%

G0S2 inhibits adipogenesis and unsaturated fatty acid biosynthesis by repressing the expression of SCD in chicken

Yang

Wang

2023

Int J of Food Sci Tech

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Fatty acids metabolic products and intramuscular fat (IMF) content determine meat quality in chickens. In this study, the mechanisms of G0/G1 switch gene 2 (G0S2) underlying modulation of chicken IMF content as well as fatty acid synthesis was investigated. Several advanced techniques including Gas Chromatography-Mass Spectrometer (GC-MS), RNA-sequencing and lentiviral transfection were utilised. RNA-sequencing showed that 2242 differentially expressed genes were significantly different, and many of these were related to lipid catabolism, such as stearoyl-CoA desaturase 1 (SCD), acyl-CoA synthetase bubblegum family member 2 (ACSBG2), and fatty acid desaturase 2 (FADS2), etc. Knocking down of G0S2 also affected the biological activities of signal transduction, catalytic activity, lipid metabolism, cell cycle and death, cell differentiation and immune response. GC-MS revealed that decreased lipid accumulation and lower unsaturated fatty acid percentage were observed in G0S2 knockdown adipocytes. Our results showed that lower expression of G0S2 decreased lipid accumulation mainly via down-regulated SCD, then regulated mRNA levels of patatin-like phospholipase domain containing 2 (PNPLA2), which was shown as a major triglyceride lipase in the liver and adipose tissue. Importantly, we further verified the regulation network of G0S2 in the thigh tissues of 12 Huangshan Black chickens, which was consistent with previous IMF results. Thus, our study highlights that G0S2 modulated chicken meat quality traits by the G0S2-SCD-PNPLA2 axis in lipid metabolism in vitro and in vivo. These findings are expected to provide a strategy from a genetic perspective for improving the meat quality.

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Early-life exposure to bisphenol A induces dysregulation of lipid homeostasis by the upregulation of SCD1 in male mice

Cited by 19 publications

References 42 publications

Serum glycolipids mediate the relationship of urinary bisphenols with NAFLD: analysis of a population-based, cross-sectional study

Serum glycolipids mediate the relationship of urinary bisphenols with NAFLD: analysis of a population-based, cross-sectional study

Comparison of metabolic differences between two geometrical isomers based on multi‐omics strategy

G0S2 inhibits adipogenesis and unsaturated fatty acid biosynthesis by repressing the expression of SCD in chicken

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