Early Neuronal and Glial Fate Restriction of Embryonic Neural Stem Cells

Delaunay, Delphine; Heydon, Katharina; Cumano, Ana; Schwab, Markus H.; Thomas, Jean‐Léon; Suter, Ueli; Nave, Klaus‐Armin; Zalc, Bernard; Spassky, Nathalie

doi:10.1523/jneurosci.5497-07.2008

Cited by 59 publications

(58 citation statements)

References 80 publications

(82 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The proximal promoter sequence extending to Ϫ2.5 kb has no independent targeting ability (Li et al, 2002), but addition of the wmN1 and wmN2 containing intron 1 sequence leads to strong transgene expression in both oligodendrocytes and Schwann cells (Mallon et al, 2002). A Cre-containing transgene regulated by plp/DM20 sequences extending 5Ј to Ϫ3.74 kb and the same intron 1 sequence demonstrated a similar expression program (Delaunay et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Consequently, plp/DM20 regulation has been investigated extensively, and long sequence domains capable of directing expression to oligodendrocytes, Schwann cells, and neuronal precursors have been identified (Nadon et al, 1994;Readhead et al, 1994;Fuss et al, 2000;Li et al, 2002;Mallon et al, 2002;Delaunay et al, 2008). However, the location and function of plp/DM20 enhancers remain essentially unknown.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Separate Proteolipid Protein/DM20 Enhancers Serve Different Lineages and Stages of Development

Tuason¹,

Rastikerdar²,

Kuhlmann³

et al. 2008

Journal of Neuroscience

Self Cite

View full text Add to dashboard Cite

The gene encoding DM20 emerged in cartilaginous fish, descending from a bilaterian ancestor of the M6 proteolipid gene family. Its proteolipid protein (PLP) isoform appeared in amphibians, contains an additional 35 amino acids, and, in the mammalian CNS, is the dominant myelin protein in which it confers an essential neuroprotective function. During development, the DM20 isoform is prominent in a number of tissues, and plp/DM20 transcripts are detected in multiple progenitor populations, including those that continue to express plp/DM20 as they differentiate into myelinating oligodendrocytes. The locus also encodes isoforms with extended leader sequences that accumulate in the cell bodies of several types of neurons. Here, to locate and characterize regulatory sequences controlling the complex plp/DM20 transcription program, putative regulatory sequences, suggested by interspecies conservation, were ligated individually to a minimally promoted eGFPlacZ reporter gene. These constructs were inserted in single copy at a common site adjacent to the hypoxanthine-guanine phosphoribosyltransferase locus in embryonic stem cells and their in vivo expression programs were compared in transgenic mice. Most expressed developmental and cell-specific subprograms accommodated within the known expression phenotype of the endogenous plp/DM20 locus, thus defining multiple components of the combinatorial mechanism controlling its normal temporal and cell-specific program. Along with previously characterized nervous system enhancers, those described here should help expose the content and configuration of elements that are operational in multiple glial and neuronal lineages. The transgenic lines derived here also provide effective markers for multiple stages of glial and neuronal lineage progression.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Separate Proteolipid Protein/DM20 Enhancers Serve Different Lineages and Stages of Development

Tuason¹,

Rastikerdar²,

Kuhlmann³

et al. 2008

Journal of Neuroscience

Self Cite

View full text Add to dashboard Cite

show abstract

“…This mean that one NE cell begets two NE cells or one radial-glia cell gives rise to two radial glia cells. 18,40,41 As development proceeds, sequential fate restrictions take place via symmetric 42,43 and asymmetric cell divisions in the VZ and SVZ. 44 Chenn and McConnell, suggested that the symmetric and asymmetric cell divisions were associated with the cleavage orientation of progenitor cells.…”

Section: Cellular Mechanism Of Neural Cell Fate Determinationmentioning

confidence: 99%

Role of miRNAs and epigenetics in neural stem cell fate determination

Lopez-Ramirez

Nicoli

2013

Epigenetics

View full text Add to dashboard Cite

“…Glia and neurons, the major cell types of the nervous system, share a common precursor population, the NSCs (Bossing et al, 1996;Delaunay et al, 2008;Doe et al, 1998;Rivers et al, 2008;Schmidt et al, 1997). The multipotency of NSCs and their ability to be redirected towards different fates make these cells a promising tool in regenerative medicine; however, the plastic features of this initially homogeneous population needs to be fully understood.…”

Section: Introductionmentioning

confidence: 99%

Gcm/Glide-dependent conversion into glia depends on neural stem cell age, but not on division, triggering a chromatin signature that is conserved in vertebrate glia

et al. 2011

View full text Add to dashboard Cite

SUMMARYNeurons and glia differentiate from multipotent precursors called neural stem cells (NSCs), upon the activation of specific transcription factors. In vitro, it has been shown that NSCs display very plastic features; however, one of the major challenges is to understand the bases of lineage restriction and NSC plasticity in vivo, at the cellular level. We show here that overexpression of the Gcm transcription factor, which controls the glial versus neuronal fate choice, fully and efficiently converts Drosophila NSCs towards the glial fate via an intermediate state. Gcm acts in a dose-dependent and autonomous manner by concomitantly repressing the endogenous program and inducing the glial program in the NSC. Most NSCs divide several times to build the embryonic nervous system and eventually enter quiescence: strikingly, the gliogenic potential of Gcm decreases with time and quiescent NSCs are resistant to fate conversion. Together with the fact that Gcm is able to convert mutant NSCs that cannot divide, this indicates that plasticity depends on temporal cues rather than on the mitotic potential. Finally, NSC plasticity involves specific chromatin modifications. The endogenous glial cells, as well as those induced by Gcm overexpression display low levels of histone 3 lysine 9 acetylation (H3K9ac) and Drosophila CREB-binding protein (dCBP) Histone Acetyl-Transferase (HAT). Moreover, we show that dCBP targets the H3K9 residue and that high levels of dCBP HAT disrupt gliogenesis. Thus, glial differentiation needs low levels of histone acetylation, a feature shared by vertebrate glia, calling for an epigenetic pathway conserved in evolution. KEY WORDS: Neural stem cells, Glia, Drosophila, dCBP, Gcm/Glide, Histone acetylationGcm/Glide-dependent conversion into glia depends on neural stem cell age, but not on division, triggering a chromatin signature that is conserved in vertebrate glia Hakima Flici*, Berra Erkosar*, Orban Komonyi, Omer Faruk Karatas, Pietro Laneve and Angela Giangrande † DEVELOPMENT MATERIALS AND METHODS FliesFlies were raised at 25°C unless otherwise specified. w 1118 was the wild type. Transgenic lines were: UAS-gcm (one dose: F18A: 1XGcm; two doses: M24A: 2XGcm) (Bernardoni et al., 1998); lbe(K)-Gal4, UAS-GFP (Baumgardt et al., 2009); mzVum-Gal4 (Landgraf et al., 2003); apterousGal4, UAS-mRFP (Baumgardt et al., 2007); gcm 34 / CyO,twi-LacZ (Vincent et al., 1996); UAS-dCBP and UAS-dCBP-FLAD (Kumar et al., 2004); repoGal4/TM3 (Sepp et al., 2001); repo 3692 /TM3ubx-lacZ (Halter et al., 1995); repo-Gal4 (Lee and Jones, 2005); and UAS-mCD8GFP, elavGal4, 4 /TM3 (Bloomington Drosophila Stock Center). Immunohistochemistry and in situ hybridizationImmunolabeling and in situ hybridization on embryos were as described previously (Bernardoni et al., 1998). Primary antibodies were: mouse(m)--Repo (1:50), m--Engrailed (1:500) and rat(rt)--Elav (1:200) from DSHB; chicken--GFP (1:1000), rabbit(rb)--RFP (1:500), rb--Caspase3 (1:500), m--H3K9ac (1:500), rb--H3K4me3 (1:500) from Abcam; rb--GFP (1...

show abstract

Early Neuronal and Glial Fate Restriction of Embryonic Neural Stem Cells

Cited by 59 publications

References 80 publications

Separate Proteolipid Protein/DM20 Enhancers Serve Different Lineages and Stages of Development

Separate Proteolipid Protein/DM20 Enhancers Serve Different Lineages and Stages of Development

Role of miRNAs and epigenetics in neural stem cell fate determination

Gcm/Glide-dependent conversion into glia depends on neural stem cell age, but not on division, triggering a chromatin signature that is conserved in vertebrate glia

Contact Info

Product

Resources

About