Early postmortem biochemical and histopathological changes in the kidney, liver, and muscles of dogs

Yahia, Doha; El-Amir, Yasmin O.; Sadek, Ahmed Abdelrahiem

doi:10.1007/s00580-018-2756-8

Cited by 5 publications

(2 citation statements)

References 11 publications

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“…Considering that no decrease in stiffness was seen and cell walls were still visible in the histological images at 10 h pm, we hypothesize that the observed increase in water diffusivity is largely due to a gradual increase in cell membrane permeability rather than cell collapse and autolysis. Increased membrane permeability with a loss of membrane integrity is usually a process of tissue degradation which is followed by cell shrinkage and cell death ( Majno et al, 1960 ; Kimura and Abe, 1994 ; Milroy, 1999 ; Yoon et al, 2002 ; Malhi et al, 2006 ; Miller and Zachary, 2017 ; Yahia et al, 2018 ). Diffusivity started to increase earlier in NPLs, indicating faster tissue degradation in NPLs than PLs.…”

Section: Discussionmentioning

confidence: 99%

Effect of Post-mortem Interval and Perfusion on the Biophysical Properties of ex vivo Liver Tissue Investigated Longitudinally by MRE and DWI

et al. 2021

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Structural changes of soft tissues on the cellular level can be characterized by histopathology, but not longitudinally in the same tissue. Alterations of cellular structures and tissue matrix are associated with changes in biophysical properties which can be monitored longitudinally by quantitative diffusion-weighted imaging (DWI) and magnetic resonance elastography (MRE). In this work, DWI and MRE examinations were performed in a 0.5-Tesla compact scanner to investigate longitudinal changes in water diffusivity, stiffness and viscosity of ex-vivo rat livers for up to 20 h post-mortem (pm). The effect of blood on biophysical parameters was examined in 13 non-perfused livers (containing blood, NPLs) and 14 perfused livers (blood washed out, PLs). Changes in cell shape, cell packing and cell wall integrity were characterized histologically. In all acquisitions, NPLs presented with higher shear-wave speed (c), higher shear-wave penetration rate (a) and smaller apparent-diffusion-coefficients (ADCs) than PL. Time-resolved analysis revealed three distinct phases: (i) an initial phase (up to 2 h pm) with markedly increased c and a and reduced ADCs; (ii) an extended phase with relatively stable values; and (iii) a degradation phase characterized by significant increases in a (10 h pm in NPLs and PLs) and ADCs (10 h pm in NPLs, 13 h pm in PLs). Histology revealed changes in cell shape and packing along with decreased cell wall integrity, indicating tissue degradation in NPLs and PLs 10 h pm. Taken together, our results demonstrate that the biophysical properties of fresh liver tissue rapidly change within 2 h pm, which seems to be an effect of both cytotoxic edema and vascular blood content. Several hours later, disruption of cell walls resulted in higher water diffusivity and wave penetration. These results reveal the individual contributions of vascular components and cellular integrity to liver elastography and provide a biophysical, imaging-based fingerprint of liver tissue degradation.

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Section: Discussionmentioning

confidence: 99%

Effect of Post-mortem Interval and Perfusion on the Biophysical Properties of ex vivo Liver Tissue Investigated Longitudinally by MRE and DWI

et al. 2021

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“…Due to the medico-legal limitations related to the assessment of the classical triad of postmortem changes (rigor mortis, livor mortis, and algor mortis), methods from various fields, such as chemistry and biochemistry, histopathology and immunohistochemistry, molecular biology, and entomology have been used to assess the PMI [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 ]. At the same time, tissues and organs, mainly the heart, liver, kidney, blood, and other body fluids have been the subject of research.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Animal Model for Estimating the Time since Death with Attention to Early Postmortem Stage

et al. 2022

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Estimating the postmortem interval (PMI) has remained the subject of investigations in forensic medicine for many years. Every kind of death results in changes in metabolites in body tissues and fluids due to lack of oxygen, altered circulation, enzymatic reactions, cellular degradation, and cessation of anabolic production of metabolites. Metabolic changes may provide markers determining the time since death, which is challenging in current analytical and observation-based methods. The study includes metabolomics analysis of blood with the use of an animal model to determine the biochemical changes following death. LC-MS is used to fingerprint postmortem porcine blood. Metabolites, significantly changing in blood after death, are selected and identified using univariate statistics. Fifty-one significant metabolites are found to help estimate the time since death in the early postmortem stage. Hypoxanthine, lactic acid, histidine, and lysophosphatidic acids are found as the most promising markers in estimating an early postmortem stage. Selected lysophosphatidylcholines are also found as significantly increased in blood with postmortal time, but their practical utility as PMI indicators can be limited due to a relatively low increasing rate. The findings demonstrate the great potential of LC-MS-based metabolomics in determining the PMI due to sudden death and provide an experimental basis for applying this attitude in investigating various mechanisms of death. As we assume, our study is also one of the first in which the porcine animal model is used to establish PMI metabolomics biomarkers.

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Histobiochemical changes in early postmortem interval in liver, pancreas, skin and kidney of adult male albino rats

2022

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Early postmortem biochemical and histopathological changes in the kidney, liver, and muscles of dogs

Cited by 5 publications

References 11 publications

Effect of Post-mortem Interval and Perfusion on the Biophysical Properties of ex vivo Liver Tissue Investigated Longitudinally by MRE and DWI

Effect of Post-mortem Interval and Perfusion on the Biophysical Properties of ex vivo Liver Tissue Investigated Longitudinally by MRE and DWI

In Vitro Animal Model for Estimating the Time since Death with Attention to Early Postmortem Stage

Histobiochemical changes in early postmortem interval in liver, pancreas, skin and kidney of adult male albino rats

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