Early postnatal administration of 5,7-dihydroxytryptamine: Effects on substance P and thyrotropin-releasing hormone neurons and terminals in rat brain

Towle, Andrew C.; Breese, George R.; Mueller, Robert A.; Hunt, Richard A.; Lauder, Jean M.

doi:10.1016/0006-8993(86)90656-6

Cited by 20 publications

(2 citation statements)

References 36 publications

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“…Indeed, 5-HT immunoreactivity is detectable in B3 as well as in BUB2 approximately 2 days later than in the presumed anlage of B4-9. Furthermore, in contrast with B4-9, 81-3 neurons mainly project to the spinal cord (Aitken, 1987), and B1-3 seem to be quite particular in that at least some cells of this complex coexpress thyrotropin-releasing hormone and/or substance P (Johansson et al, 1981;Foster et al, 1985;Towle et al, 1986).…”

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confidence: 92%

Tyrosine hydroxylase and serotonin containing cells in embryonic rat rhombencephalon: A whole‐mount immunocytochemical study

König

Wilkie

Lauder

1988

J of Neuroscience Research

109

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Rhombencephala from rat embryos were processed as whole-mounts for immunocytochemical detection of monoaminergic cell populations, using antibodies to tyrosine hydroxylase (TH) and serotonin (5-HT). Specific advantages of the whole-mount technique over the classical serial-section method were that even isolated immunoreactive (IR) cells could be detected easily, and three-dimensional relationships could be ascertained without the need for serial reconstruction. Embryos between embryonic days (E) 12 and 16 (the day following nocturnal mating being considered as E1) were used in this study. Both TH and 5-HT immunoreactivities were already detectable at E12, even in the smallest embryos (crown-rump length: 6 mm), but there was a striking difference in the number and regional distribution of these two types of IR cells. TH was expressed in several cell groups located in the rostral rhombencephalon (the presumed anlage of the A4-7 complex) as well as in the caudal rhombencephalon (the presumed anlagen of groups A1-2 and C1-3), whereas 5-HT was expressed in very few cells located near the rostral border of the rhombencephalon (presumed anlage of the B4-9 complex). Although the three-dimensional distribution of the TH-IR cell groups underwent some modifications during the period studied, its general pattern remained relatively stable after E12. This contrasted with the sequential appearance of the 5-HT-IR cell groups and their spatial transformations during this period. Using the rhombencephalic isthmus as a landmark, we found that conspicuous 5-HT-IR fibre bundles penetrated into the mesencephalon from E13 onwards, but that the 5-HT IR cell bodies were exclusively located caudal to the borderline between the mesencephalon and the rhombencephalon (the rhombencephalic isthmus). We therefore suggest the term "rostral rhombencephalic raphe nuclei" for the rostral 5-HT cell groups instead of "mesencephalic raphe nuclei," which is a misnomer. Close spatial association between TH and 5-HT-IR elements was observed mainly in the caudal rhombencephalon, where 5-HT-IR fibres coursed through an area containing numerous TH-IR cell bodies (the presumed anlagen of groups A1-2 and C1-3).

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confidence: 92%

Tyrosine hydroxylase and serotonin containing cells in embryonic rat rhombencephalon: A whole‐mount immunocytochemical study

König

Wilkie

Lauder

1988

J of Neuroscience Research

109

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“…(König et al, 1989a;Rajaofetra et al, 1992). In addition, these transplanted specific neuron populations exhibited in their terminals both serotonin and substance P as described in intact animals (Johansson et al, 1981;Foster et al, 1985;Towle et al, 1986;Kachidian et al, 1991;Rajaofetra et al, 1991). However, the mechanisms by which axons of these transplanted embryonic neurons reach their specific final position in adult animals remain largely unknown.…”

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confidence: 99%

Embryonic raphe neurons grafted in vitro on fetal spinal cord slices recognize specific target areas

Ribotta

König

1999

J. Neurosci. Res.

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To investigate the characteristics of neurotropic signaling involved in specific target recognition by grafted embryonic serotonergic cells, we have developed an in vitro grafting model. Specific raphe nuclei (B1/B2 and B3) were respectively dissected from 14-day-old rat embryos, and partially dissociated cells were cocultured on spinal cord slices from 20-day-old fetuses. After serotonin immunodetection, neurite growth patterns were analyzed by standard photonic or confocal scanning microscopy. Computer reconstruction (maximal signal projection) was used to track individual neurites in spite of their changing depth levels. Whereas the direction and branching of the initial neurite segments did not seem to be significantly influenced by any specific environment, specific growth patterns were developed at some distance from the cell bodies, indicating that neurites are able to recognize their specific targets.

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Cellular localization of substance P‐ and neurokinin A‐encoding preprotachykinin mRna in the female rat brain

Harlan

Garcia

Krause

1989

J of Comparative Neurology

104

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To determine the locations of neurons in the rat brain expressing substance P and neurokinin A mRNA, we performed in situ hybridization with a radiolabeled cRNA probe that was complementary to alpha-, beta-, and gamma-preprotachykinin mRNA. Several types of controls indicated specificity of the labeling. Brain regions containing many labeled neurons include the anterior olfactory nucleus, layer II of the olfactory tubercle, the islands of Calleja, the nucleus accumbens, the caudate-putamen, portions of the amygdala and hypothalamus, the medial habenular nucleus, nuclei of the pontine tegmentum, several raphe nuclei, several portions of the reticular formation, and the nucleus of the solitary tract. Less frequent labeled neurons were also found in many other regions of the brain. These results extend many previous immunocytochemical studies of the locations of neurons containing immunoreactive substance P, neurokinin A, and neuropeptide K.

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Early postnatal administration of 5,7-dihydroxytryptamine: Effects on substance P and thyrotropin-releasing hormone neurons and terminals in rat brain

Cited by 20 publications

References 36 publications

Tyrosine hydroxylase and serotonin containing cells in embryonic rat rhombencephalon: A whole‐mount immunocytochemical study

Tyrosine hydroxylase and serotonin containing cells in embryonic rat rhombencephalon: A whole‐mount immunocytochemical study

Embryonic raphe neurons grafted in vitro on fetal spinal cord slices recognize specific target areas

Cellular localization of substance P‐ and neurokinin A‐encoding preprotachykinin mRna in the female rat brain

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