Early responses of osteoblast-like cells to different mechanical signals through various signaling pathways

Li, Jun; Liu, Tingting; Zheng, Yi; Zhao, Zhihe; Liu, Ying; Cheng, Hui; Luo, Sushan; Chen, Yangxi

doi:10.1016/j.bbrc.2006.07.175

Cited by 74 publications

(65 citation statements)

References 27 publications

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“…The force-loading plates were made by using a method described previously (18). The cells were incubated in DMEM supplemented with 10% FBS and 1% penicillin/streptomycin for 48 h. The medium was then changed to FBS-free medium for 24 h for equalizing cell growth prior to experiments.…”

Section: Applying Mechanical Stressmentioning

confidence: 99%

“…The cells were incubated in DMEM supplemented with 10% FBS and 1% penicillin/streptomycin for 48 h. The medium was then changed to FBS-free medium for 24 h for equalizing cell growth prior to experiments. The cells were then loaded with tensile stress at 2000 u-strain and 0.5 Hz, which has been characterized and described in detail elsewhere (18). In the control group, cells were subjected to similar treatment but without mechanical stress stimulus.…”

Section: Applying Mechanical Stressmentioning

confidence: 99%

“…After the cells were harvested, total RNA was extracted and quantified using the method described previously (18). RNA was reverse-transcribed using the First Strand cDNA Synthesis Kit following the manufacturer's instructions.…”

Section: Rna Isolation and Real-time Pcrmentioning

confidence: 99%

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Insulin effect on RANKL and OPG expression in human osteoblast-like MG63 cells

Wang¹,

Zhong²

2013

Turk J Med Sci

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A simple method for simultaneous pre-concentration of Cu, Ni, and Co metal ions prior to their determination by flame atomic absorption spectrometry is reported. The method is based on the extraction of target ions via complexation with indane-1,2,3-trione 1,2-dioxime immobilized on sodium dodecyl sulfate (SDS) coated alumina. The metal content on the adsorbent was eluted with 4.5 mL of 2 mol L −1 nitric acid. The influences of the analytical parameters including pH, sample volume and flow rate, and eluent type and flow rate were investigated. The effect of matrix ions on the retentions of the analytes was also examined. The recoveries of analytes were generally higher than 96% with a low relative standard deviation (R.S.D. < 3%). The presented method was successfully applied for determination of these metals in water samples.

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Section: Applying Mechanical Stressmentioning

confidence: 99%

Section: Applying Mechanical Stressmentioning

confidence: 99%

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Insulin effect on RANKL and OPG expression in human osteoblast-like MG63 cells

Wang¹,

Zhong²

2013

Turk J Med Sci

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“…Mechanical stress was applied following published techniques in which MAPK signal transduction was induced by mechanical stress. 10,11 Cells were stretched with a 10% biaxial strain at a frequency of 30 times/min for 14 hours with a cell strain unit (FX-3000, Flexcell International, Hillsborough, NC, USA).…”

Section: Mechanical Stress Constructionmentioning

confidence: 99%

“…They predicted that following cytoskeletal rearrangement induced by mechanical stress, ERK1/2 are phosphorylated and downstream transcriptional factors are expressed. 10 However, the mechanism of CYP24 expression is not clear with regard to osteoblasts under mechanical stress. In this study, we investigated CYP24 promoter activity in osteoblastic cells that were stretched and inhibited by ERK1/2 or p38, typical transmitters in the MAPK superfamily.…”

Section: Introductionmentioning

confidence: 99%

CYP24 Promoter Activity is Affected by Mechanical Stress and Mitogen-Activated Protein Kinase in MG63 Osteoblast-like Cells

Toyoshita

Iida

Koshino

et al. 2008

J Jpn Prosthodont Soc

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Bone grafts and improvement in bone quality will increase in the future. Thus, molecular biological knowledge of bone metabolism is essential for prosthetic therapy. This study reveals that expression of CYP24, which inactivates vitamin D, is controlled by mechanical stress and mitogen-activated protein kinase. AbstractPurpose: For bone homeostasis, vitamin D plays an important role in the regulation of calcium. The enzyme CYP24 inactivates vitamin D and is involved in its regulation. However, the mechanism of expression of CYP24 in osteoblastic cells under mechanical stress is not clear. In this study we investigated CYP24 promoter activity in stretched osteoblastic cells and the participation of mitogen-activated protein kinase (MAPK) in expression of CYP24.Methods: MG63 osteoblastic cells were cultured on silicon-bottomed plates. Cells were transfected with a reporter gene that contained a CYP24 promoter. After activated vitamin D, 1,25(OH) 2 D 3 , was added or not added, cells were stretched. Stretched and non-stretched cells were investigated by luciferase dual assay. Cells were also investigated similarly using medium with an ERK1/2 inhibitor or p38 inhibitor. Results:The CYP24 promoter was activated by 1,25(OH) 2 D 3 and the promoter activity decreased in stretched cells. Inhibitor of MAPK decreased CYP24 promoter activity. However, CYP24 promoter activity decreased with mechanical stress after addition of p38 inhibitor, while it did not decrease with mechanical stress after addition of ERK1/2 inhibitor. The CYP24 promoter was not activated without 1,25(OH) 2 D 3 in any case. Conclusion:Mechanical stress and MAPK control CYP24 promoter activity in the presence of Vitamin D in MG63 osteoblast-like cells.

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Fluid shear stress induces Runx‐2 expression via upregulation of PIEZO1 in MC3T3‐E1 cells

Song

Liu

et al. 2020

Cell Biology International

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Mechanically induced biological responses in bone cells involve a complex biophysical process. Although various mechanosensors have been identified, the precise mechanotransduction pathway remains poorly understood. PIEZO1 is a newly discovered mechanically activated ion channel in bone cells. This study aimed to explore the involvement of PIEZO1 in mechanical loading (fluid shear stress)‐induced signaling cascades that control osteogenesis. The results showed that fluid shear stress increased PIEZO1 expression in MC3T3‐E1 cells. The fluid shear stress elicited the key osteoblastic gene Runx‐2 expression; however, PIEZO1 silencing using small interference RNA blocked these effects. The AKT/GSK‐3β/β‐catenin pathway was activated in this process. PIEZO1 silencing impaired mechanically induced activation of the AKT/GSK‐3β/β‐catenin pathway. Therefore, the results demonstrated that MC3T3‐E1 osteoblasts required PIEZO1 to adapt to the external mechanical fluid shear stress, thereby inducing osteoblastic Runx‐2 gene expression, partly through the AKT/GSK‐3β/β‐catenin pathway.

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Early responses of osteoblast-like cells to different mechanical signals through various signaling pathways

Cited by 74 publications

References 27 publications

Insulin effect on RANKL and OPG expression in human osteoblast-like MG63 cells

Insulin effect on RANKL and OPG expression in human osteoblast-like MG63 cells

CYP24 Promoter Activity is Affected by Mechanical Stress and Mitogen-Activated Protein Kinase in MG63 Osteoblast-like Cells

Fluid shear stress induces Runx‐2 expression via upregulation of PIEZO1 in MC3T3‐E1 cells

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