Early-stage cervical cancer diagnosis based on an ultra-sensitive electrochemical DNA nanobiosensor for HPV-18 detection in real samples

Mahmoodi, Pegah; Rezayi, Majid; Rasouli, Elisa; Gholami, Mehrdad; Karimi, Ehsan; Alias, Yatima

doi:10.1186/s12951-020-0577-9

Cited by 69 publications

(47 citation statements)

References 49 publications

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“…A recent study suggested the feasibility of using nanotechnology to detect HPV DNA. This could be an experimental diagnostic tool in the future [ 182 , 183 ].…”

Section: Hpv Screeningmentioning

confidence: 99%

Current Updates on Cancer-Causing Types of Human Papillomaviruses (HPVs) in East, Southeast, and South Asia

Xia

Long

et al. 2021

Cancers

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Human papillomavirus (HPV) infection remains one of the most prominent cancer-causing DNA viruses, contributing to approximately 5% of human cancers. While association between HPV and cervical cancers has been well-established, evidence on the attribution of head and neck cancers (HNC) to HPV have been increasing in recent years. Among the cancer-causing HPV genotypes, HPV16 and 18 remain the major contributors to cancers across the globe. Nonetheless, the distribution of HPV genotypes in ethnically, geographically, and socio-economically diverse East, Southeast, and South Asia may differ from other parts of the world. In this review, we garner and provide updated insight into various aspects of HPV reported in recent years (2015–2021) in these regions. We included: (i) the HPV genotypes detected in normal cancers of the uterine cervix and head and neck, as well as the distribution of the HPV genotypes by geography and age groups; (ii) the laboratory diagnostic methods and treatment regimens used within these regions; and (iii) the oncogenic properties of HPV prototypes and their variants contributing to carcinogenesis. More importantly, we also unveil the similarities and discrepancies between these aspects, the areas lacking study, and the challenges faced in HPV studies.

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“…A recent study suggested the feasibility of using nanotechnology to detect HPV DNA. This could be an experimental diagnostic tool in the future [ 182 , 183 ].…”

Section: Hpv Screeningmentioning

confidence: 99%

Current Updates on Cancer-Causing Types of Human Papillomaviruses (HPVs) in East, Southeast, and South Asia

Xia

Long

et al. 2021

Cancers

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“…In order to make the surface of the SPCE as homogenous as possible, it is important that they are pre-treated. These pre-treatments remove any binder residues left on the carbon surface after the curing process [ 26 , 27 , 28 , 29 ], with well-established electrochemical oxidative pre-treatments not only showing removal of binder residues left on the surface of SPCE after curing but also improvement of carbon surface sensitivity [ 30 ]. In this study, two common buffers are compared for pre-treatment: NaOH and NaCl.…”

Section: Introductionmentioning

confidence: 99%

Optimisation of an Electrochemical DNA Sensor for Measuring KRAS G12D and G13D Point Mutations in Different Tumour Types

et al. 2021

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Circulating tumour DNA (ctDNA) is widely used in liquid biopsies due to having a presence in the blood that is typically in proportion to the stage of the cancer and because it may present a quick and practical method of capturing tumour heterogeneity. This paper outlines a simple electrochemical technique adapted towards point-of-care cancer detection and treatment monitoring from biofluids using a label-free detection strategy. The mutations used for analysis were the KRAS G12D and G13D mutations, which are both important in the initiation, progression and drug resistance of many human cancers, leading to a high mortality rate. A low-cost DNA sensor was developed to specifically investigate these common circulating tumour markers. Initially, we report on some developments made in carbon surface pre-treatment and the electrochemical detection scheme which ensure the most sensitive measurement technique is employed. Following pre-treatment of the sensor to ensure homogeneity, DNA probes developed specifically for detection of the KRAS G12D and G13D mutations were immobilized onto low-cost screen printed carbon electrodes using diazonium chemistry and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride/N-hydroxysuccinimide coupling. Prior to electrochemical detection, the sensor was functionalised with target DNA amplified by standard and specialist PCR methodologies (6.3% increase). Assay development steps and DNA detection experiments were performed using standard voltammetry techniques. Sensitivity (as low as 0.58 ng/μL) and specificity (>300%) was achieved by detecting mutant KRAS G13D PCR amplicons against a background of wild-type KRAS DNA from the representative cancer sample and our findings give rise to the basis of a simple and very low-cost system for measuring ctDNA biomarkers in patient samples. The current time to receive results from the system was 3.5 h with appreciable scope for optimisation, thus far comparing favourably to the UK National Health Service biopsy service where patients can wait for weeks for biopsy results.

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“…Herein, a dendritic polymer probe-amplified electrochemiluminescence (ECL) [40][41][42] scan imaging system was constructed to realize trace analysis of viral emerging infectious disease (Figure 1). Dendritic polymer probe was employed as the efficient signal giving-out component that could generate amplified ECL signal on the integrated chip.…”

Section: Introductionmentioning

confidence: 99%

Trace Analysis of Emerging Virus: an Ultrasensitive ECL-Scan Imaging System for Viral Infectious Disease

Wu¹,

Wu²,

Fu³

et al. 2021

Preprint

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BackgroundEmerging infectious disease have brought a huge impact on human society in recent years. The outbreak of Zika virus (ZIKV) in the Americas resulted in a large number of babies born with microcephaly. More seriously, the Coronavirus Disease 2019 (COVID-19) caused the global spreads and immeasurable damages. Thus, the monitoring of highly pathogenic virus is of significance to the prevention and control of emerging infectious disease.ResultsHerein, a dendritic polymer probe-amplified ECL-scan imaging system was constructed to realize trace analysis of viral emerging infectious disease. Dendritic polymer probe was employed as the efficient signal giving-out component that could generate amplified electrochemiluminescence (ECL) signal on the integrated chip. And the signal was detected by a single-photon level charge coupled device-based ECL-scan imaging system. With this strategy,the ZIKV in the complex system of blood, urine and saliva were detected. The results indicated that high sensitivity of 50 copies and superior specificity were achieved. Furthermore, this strategy realized highly sensitive detection (10 copies) of S and N protein gene sequence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-Cov2) and spiked pseudovirus samples.ConclusionsThus, the dendritic polymer probe-amplified ECL-scan imaging system suitably met the strict clinical-requirements for trace analysis of emerging virus, and thus has the potential to serve as a paradigm for monitoring of emerging infectious disease.

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Early-stage cervical cancer diagnosis based on an ultra-sensitive electrochemical DNA nanobiosensor for HPV-18 detection in real samples

Cited by 69 publications

References 49 publications

Current Updates on Cancer-Causing Types of Human Papillomaviruses (HPVs) in East, Southeast, and South Asia

Current Updates on Cancer-Causing Types of Human Papillomaviruses (HPVs) in East, Southeast, and South Asia

Optimisation of an Electrochemical DNA Sensor for Measuring KRAS G12D and G13D Point Mutations in Different Tumour Types

Trace Analysis of Emerging Virus: an Ultrasensitive ECL-Scan Imaging System for Viral Infectious Disease

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