Early stage of cytomegalovirus infection suppresses host microRNA expression regulation in human fibroblasts

Buzdin, Anton; Artcibasova, Alina V.; Fedorova, Natalya F; Suntsova, Maria; Garazha, Andrew; Sorokin, Maxim; Allina, Daria; Shalatonin, M. P.; Borisov, Nicolas; Zhavoronkov, Alex; Kovalchuk, Igor; Kovalchuk, Olga; Кущ, А А

doi:10.1080/15384101.2016.1241928

Cited by 19 publications

(7 citation statements)

References 43 publications

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“…We collected, analyzed, and preprocessed data from experimentally validated microRNA target databases miRTarBase [ 56 ] and Diana TarBase [ 57 ] according to [ 58 ] to add microRNA–mRNA interaction data to the MiRImpact operational database. For miPAS calculations, we treated microRNA concentrations in FTC as the “case” and in FA as the “normal” values.…”

Section: Methodsmentioning

confidence: 99%

Reciprocal Dysregulation of MiR-146b and MiR-451 Contributes in Malignant Phenotype of Follicular Thyroid Tumor

Knyazeva

Korobkina

Karizky

et al. 2020

IJMS

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Over the last few years, incidental thyroid nodules are being diagnosed with increasing frequency with the use of highly sensitive imaging techniques. The ultrasound thyroid gland examination, followed by the fine-needle aspiration cytology is the standard diagnostic approach. However, in cases of the follicular nature of nodules, cytological diagnosis is not enough. Analysis of miRNAs in the biopsy presents a promising approach. Increasing our knowledge of miRNA’s role in follicular carcinogenesis, and development of the appropriate the miRNA analytical technologies are required to implement miRNA-based tests in clinical practice. We used material from follicular thyroid nodes (n.84), grouped in accordance with their invasive properties. The invasion-associated miRNAs expression alterations were assayed. Expression data were confirmed by highly sensitive two-tailed RT-qPCR. Reciprocally dysregulated miRNAs pair concentration ratios were explored as a diagnostic parameter using receiver operation curve (ROC) analysis. A new bioinformatics method (MiRImpact) was applied to evaluate the biological significance of the observed expression alterations. Coupled experimental and computational approaches identified reciprocal dysregulation of miR-146b and miR-451 as important attributes of follicular cell malignant transformation and follicular thyroid cancer progression. Thus, evaluation of combined dysregulation of miRNAs relevant to invasion and metastasis can help to distinguish truly malignant follicular thyroid cancer from indolent follicular adenoma.

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Section: Methodsmentioning

confidence: 99%

Reciprocal Dysregulation of MiR-146b and MiR-451 Contributes in Malignant Phenotype of Follicular Thyroid Tumor

Knyazeva

Korobkina

Karizky

et al. 2020

IJMS

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“…Kim et al (BioProject PRJNA269099) found that a large fraction of human miRNAs targets was shared with viral miRNAs in HFFs infected with Towne varL after 24, 48 and 72 h post infection [ 99 ]. In 2016, Buzdin and colleagues (BioProject PRJNA304028) could link a complete suppression of host miRNAs regulation during early stages (3 h) of an HCMV infection, by infecting embryonic lung fibroblasts (HELF-977) and skin fibroblasts (HAF-1608) with AD169 [ 98 ]. Lastly, Stark et al found evidence of miRNAs being derived from the lncRNA RNA2.7, contributing to profile HCMV long RNAs as precursors to other functional RNAs.…”

Section: Next-generation Sequencing In Hcmv Researchmentioning

confidence: 99%

Human cytomegalovirus genomics and transcriptomics through the lens of next-generation sequencing: revision and future challenges

Martí-Carreras

Maes

2019

Virus Genes

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The human cytomegalovirus (HCMV) genome was sequenced by hierarchical shotgun almost 30 years ago. Over these years, low and high passaged strains have been sequenced, improving, albeit still far from complete, the understanding of the coding potential, expression dynamics and diversity of wild-type HCMV strains. Next-generation sequencing (NGS) platforms have enabled a huge advancement, facilitating the comparison of differentially passaged strains, challenging diagnostics and research based on a single or reduced gene set genotyping. In addition, it allowed to link genetic features to different viral phenotypes as for example, correlating large genomic rearrangements to viral attenuation or different mutations to antiviral resistance and cell tropism. NGS platforms provided the first high-resolution experiments to HCMV dynamics, allowing the study of intra-host viral population structures and the description of rare transcriptional events. Long-read sequencing has recently become available, helping to identify new genomic rearrangements , partially accounting for the genetic variability displayed in clinical isolates, as well as, in changing the understanding of the HCMV transcriptome. Better knowledge of the transcriptome resulted in a vast number of new splicing events and alternative transcripts, although most of them still need additional validation. This review summarizes the sequencing efforts reached so far, discussing its approaches and providing a revision and new nuances on HCMV sequence variability in the sequencing field.

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“…We found a decreased expression of miR-200b-3p and miR-200c-3p in pre-transplant peripheral mononuclear cells stimulated using a laboratory HCMV strain in recipients with post-SOT HCMV infection or disease and in HCMV-infected lytic end-organ tissues [ 35 , 40 ]. One study has found a significantly decreased expression of miR-200b-3p and miR-200c-3p in HCMV AD169-infected fibroblasts compared with that in mock-treated cells [ 41 ]. In contrast, other studies found that miR-200b-3p and miR-200c-3p are not differentially regulated in cells latently infected with HCMV [ 28 , 42 ].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, we evaluated several miRNAs that potentially impact IE72 synthesis by identifying candidate molecules that bound to the 3′-UTR of UL123 by subjecting argonaute-crosslinking immunoprecipitation (AGO-CLIP)-seq and mRNA-seq data to bioinformatic prediction [ 27 ]. This indicated that miR-200b-3p and miR-200c-3p play principal roles in host protective mechanisms during lytic replication [ 23 , 34 , 35 , 40 , 41 , 58 , 59 ]. We investigated changes in the expression of MIE proteins and HCMV viral load in response to miR-200b-3p and miR-200c-3p upregulation during the early stage of active HCMV replication and latent infection.…”

Section: Introductionmentioning

confidence: 99%

Human MicroRNAs Attenuate the Expression of Immediate Early Proteins and HCMV Replication during Lytic and Latent Infection in Connection with Enhancement of Phosphorylated RelA/p65 (Serine 536) That Binds to MIEP

Hong

Min

Kim

et al. 2022

IJMS

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Attenuating the expression of immediate early (IE) proteins is essential for controlling the lytic replication of human cytomegalovirus (HCMV). The human microRNAs (hsa-miRs), miR-200b-3p and miR-200c-3p, have been identified to bind the 3′-untranslated region (3′-UTR) of the mRNA encoding IE proteins. However, whether hsa-miRs can reduce IE72 expression and HCMV viral load or exhibit a crosstalk with the host cellular signaling machinery, most importantly the NF-κB cascade, has not been evaluated. In this study, argonaute-crosslinking and immunoprecipitation-seq revealed that miR-200b-3p and miR-200c-3p bind the 3′-UTR of UL123, which is a gene that encodes IE72. The binding of these miRNAs to the 3′-UTR of UL123 was verified in transfected cells stably expressing GFP. We used miR-200b-3p/miR-200c-3p mimics to counteract the downregulation of these miRNA after acute HCMV infection. This resulted in reduced IE72/IE86 expression and HCMV VL during lytic infection. We determined that IE72/IE86 alone can inhibit the phosphorylation of RelA/p65 at the Ser536 residue and that p-Ser536 RelA/p65 binds to the major IE promoter/enhancer (MIEP). The upregulation of miR-200b-3p and miR-200c-3p resulted in the phosphorylation of RelA/p65 at Ser536 through the downregulation of IE, and the binding of the resultant p-Ser536 RelA/p65 to MIEP resulted in a decreased production of pro-inflammatory cytokines. Overall, miR-200b-3p and miR-200c-3p—together with p-Ser536 RelA/p65—can prevent lytic HCMV replication during acute and latent infection.

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Early stage of cytomegalovirus infection suppresses host microRNA expression regulation in human fibroblasts

Cited by 19 publications

References 43 publications

Reciprocal Dysregulation of MiR-146b and MiR-451 Contributes in Malignant Phenotype of Follicular Thyroid Tumor

Reciprocal Dysregulation of MiR-146b and MiR-451 Contributes in Malignant Phenotype of Follicular Thyroid Tumor

Human cytomegalovirus genomics and transcriptomics through the lens of next-generation sequencing: revision and future challenges

Human MicroRNAs Attenuate the Expression of Immediate Early Proteins and HCMV Replication during Lytic and Latent Infection in Connection with Enhancement of Phosphorylated RelA/p65 (Serine 536) That Binds to MIEP

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