Early-stage performance evaluation of flowing microbial fuel cells using chemically treated carbon felt and yeast biocatalyst

Christwardana, Marcelinus; Frattini, Domenico; Accardo, Grazia; Yoon, Sung Pil; Kwon, Yongchai

doi:10.1016/j.apenergy.2018.03.193

Cited by 54 publications

(15 citation statements)

References 67 publications

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“…DNA extraction and sequencing can be useful when analyzing biofilms grown from a consortium, yet it requires invasive methods such as the removal of parts of the biofilm or sections of the electrode [23,24]. Optical microscopy can also be used to obtain information on the localization of the biofilm, yet it is limited as to the amount of information that can be obtained [25]. MFCs are complex bio-electrochemical systems, and would therefore require more than a single technique to be completely understood.…”

Section: Introductionmentioning

confidence: 99%

On-Line Raman Spectroscopic Study of Cytochromes’ Redox State of Biofilms in Microbial Fuel Cells

et al. 2019

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Bio-electrochemical systems such as microbial fuel cells and microbial electrosynthesis cells depend on efficient electron transfer between the microorganisms and the electrodes. Understanding the mechanisms and dynamics of the electron transfer is important in order to design more efficient reactors, as well as modifying microorganisms for enhanced electricity production. Geobacter are well known for their ability to form thick biofilms and transfer electrons to the surfaces of electrodes. Currently, there are not many “on-line” systems for monitoring the activity of the biofilm and the electron transfer process without harming the biofilm. Raman microscopy was shown to be capable of providing biochemical information, i.e., the redox state of C-type cytochromes, which is integral to external electron transfer, without harming the biofilm. In the current study, a custom 3D printed flow-through cuvette was used in order to analyze the oxidation state of the C-type cytochromes of suspended cultures of three Geobacter sulfurreducens strains (PCA, KN400 and ΔpilA). It was found that the oxidation state is a good indicator of the metabolic state of the cells. Furthermore, an anaerobic fluidic system enabling in situ Raman measurements was designed and applied successfully to monitor and characterize G. sulfurreducens biofilms during electricity generation, for both a wild strain, PCA, and a mutant, ΔS. The cytochrome redox state, monitored by the Raman peak areas, could be modulated by applying different poise voltages to the electrodes. This also correlated with the modulation of current transferred from the cytochromes to the electrode. The Raman peak area changed in a predictable and reversible manner, indicating that the system could be used for analyzing the oxidation state of the proteins responsible for the electron transfer process and the kinetics thereof in-situ.

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Section: Introductionmentioning

confidence: 99%

On-Line Raman Spectroscopic Study of Cytochromes’ Redox State of Biofilms in Microbial Fuel Cells

et al. 2019

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“…In this experiment, a single chamber MFC reactor built of acrylic with an active volume of 28 mL was used to generate electrical energy, as illustrated in Figure 1. During the 21-day acclimation period, the YPD medium (5 mg/mL yeast extract, 2.5 mg/mL peptone, and 14 mg/mL D-glucose) was employed as a medium in MFC (Christwardana et al, 2018c). As a biocatalyst, 14 mg/mL of yeast Saccharomyces cerevisiae was put into the anode chamber (Christwardana et al, 2018a;Christwardana et al, 2019).…”

Section: Mfc Operationmentioning

confidence: 99%

Energy Harvesting from Sugarcane Bagasse Juice using Yeast Microbial Fuel Cell Technology

Christwardana

Yoshi

Joelianingsih

2021

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This study demonstrates the feasibility of producing bioelectricity utilizing yeast microbial fuel cell (MFC) technology with sugarcane bagasse juice as a substrate. Yeast Saccharomyces cerevisiae was employed as a bio-catalyst in the production of electrical energy. Sugarcane bagasse juice can be used as a substrate in MFC yeast because of its relatively high sugar content. When yeast was used as a biocatalyst, and Yeast Extract, Peptone, D-Glucose (YPD) Medium was used as a substrate in the MFC in the acclimatization process, current density increased over time to reach 171.43 mA/m2 in closed circuit voltage (CCV), maximum power density (MPD) reached 13.38 mW/m2 after 21 days of the acclimatization process. When using sugarcane bagasse juice as a substrate, MPD reached 6.44 mW/m2 with a sugar concentration of about 5230 ppm. Whereas the sensitivity, maximum current density (Jmax), and apparent Michaelis-Menten constant (𝐾𝑚𝑎𝑝𝑝) from the Michaelis-Menten plot were 0.01474 mA/(m2.ppm), 263.76 mA/m2, and 13594 ppm, respectively. These results indicate that bioelectricity can be produced from sugarcane bagasse juice by Saccharomyces cerevisiae.Keywords: biomass valorization, biofuel cell, acclimatization, maximum power density, Michaelis-Menten constant

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“…Notably, the SMFC from site TH1 had a significantly higher maximum power density (5.34 mW m −2 ) compared to that from site TH2 (1.82 mW m −2 ). Previous researches determined that the bioelectrochemical system of an SMFC was influenced by several operational characteristics, such as differently pretreated carbon felts, electrodes' resistance, microorganisms' growth and development, and electrode surface inhomogeneity [29,30]. The same electrodes were designed in each reaction column, so the same electrodes' resistances were obtained (5.5 Ω), which led to the speculation that the different electrical parameters from sites TH1 and TH2 might be mainly due to the microbial community composition.…”

Section: Chemical Properties Of the Original Sediments And Lake Watermentioning

confidence: 99%

Contrasting Effects of Sediment Microbial Fuel Cells (SMFCs) on the Degradation of Macrophyte Litter in Sediments from Different Areas of a Shallow Eutrophic Lake

2019

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Eutrophication is one of the major ecological problems of our era. It accelerates the growth of aquatic plant and algae, eventually leading to ecological deterioration. Based on a 700-day lab experiment, this paper investigated the contrasting effects of sediment microbial fuel cells (SMFCs) on the removal of macrophyte litter in a macrophyte-dominated area and an algae-dominated area from two bay areas of a shallow eutrophic lake. The results revealed that the removal efficiencies of total organic carbon increased by 14.4% in the macrophyte-dominated area and 7.8% in the algae-dominated area. Moreover, it was found that sediment samples from the macrophyte-dominated area became more humified and had a higher electricity generation compared to the sediment samples from the algae-dominated area. Pyrosequencing analysis further determined that SMFC promoted more aromatic compound-degrading bacteria growth in sediments from the macrophyte-dominated area than from the algae-dominated area. Our study demonstrated that SMFC could enhance organic matter degradation, especially plant litter degradation, but this influence showed different from sediment sources. Thus, SMFC is capable of providing a useful strategy for delaying the terrestrialization of lakes areas suffering from eutrophication.

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Early-stage performance evaluation of flowing microbial fuel cells using chemically treated carbon felt and yeast biocatalyst

Cited by 54 publications

References 67 publications

On-Line Raman Spectroscopic Study of Cytochromes’ Redox State of Biofilms in Microbial Fuel Cells

On-Line Raman Spectroscopic Study of Cytochromes’ Redox State of Biofilms in Microbial Fuel Cells

Energy Harvesting from Sugarcane Bagasse Juice using Yeast Microbial Fuel Cell Technology

Contrasting Effects of Sediment Microbial Fuel Cells (SMFCs) on the Degradation of Macrophyte Litter in Sediments from Different Areas of a Shallow Eutrophic Lake

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