Early use of cardiac troponin-I and echocardiography imaging for prediction of myocardial infarction size in Wistar rats

Vietta, Giovanna Grünewald; Andrades, Michael Éverton; Dall'Alba, Rafael; Schneider, Stefanie Ingrid Reis; Frick, Luzia Menegoto; Matte, Úrsula; Biolo, Andréia; Rohde, Luís Eduardo Paim; Clausell, Nadine Oliveira

doi:10.1016/j.lfs.2013.05.026

Cited by 12 publications

(11 citation statements)

References 29 publications

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“…Twelve hours after the surgery, blood was collected for cTnI evaluation as a proxy for AMI extension [22]. After that, rats were randomly assigned into either vehicle (saline, n=10) group, NAC group (25 mg/kg/day for 28 days, n=5), DFX group (40 mg/kg/day for 7 days, n=9), or NAC plus DFX group (NAC 25 mg/kg/day for 28 days plus DFX 40 mg/kg/ day for 7 days, n=9).…”

Section: Treatmentmentioning

confidence: 99%

“…After that, rats were randomly assigned into either vehicle (saline, n=10) group, NAC group (25 mg/kg/day for 28 days, n=5), DFX group (40 mg/kg/day for 7 days, n=9), or NAC plus DFX group (NAC 25 mg/kg/day for 28 days plus DFX 40 mg/kg/ day for 7 days, n=9). The choice for starting the treatments 12 h after the surgery was to avoid any acute effect on cell viability and to be able in detecting AMI plasma marker cTnI with reliability, as demonstrated before [22]. All animals were followed for 28 days, and the drugs were administered by subcutaneous injection.…”

Section: Treatmentmentioning

confidence: 99%

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N-acetylcysteine Plus Deferoxamine Improves Cardiac Function in Wistar Rats After Non-reperfused Acute Myocardial Infarction

Phaelante

Rohde

Lopes

et al. 2015

J. of Cardiovasc. Trans. Res.

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The antioxidant N-acetycysteine can turn into a prooxidant molecule in presence of iron ions. Thus, our goal was to test if the association of N-acetylcysteine (NAC) and an iron chelator (deferoxamine--DFX) in a rodent model of acute myocardial infarction (AMI) improves cardiac function. Male Wistar rats were subjected to a SHAM surgery or AMI. The animals were randomized: vehicle, NAC (25 mg/kg for 28 days), DFX (40 mg/kg for 7 days), or NAC plus DFX (NAC plus DFX, respectively). Animals were killed 28 days after the AMI. Animals treated with NAC/DFX showed an increase in left ventricular ejection fraction at 28 days when compared with vehicle group (45.2 ± 10.9 % vs. 34.7 ± 8.7 %, p = 0.03). Antioxidant effect of NAC/DFX treatment decreased 4-hydroxynonenal when compared to AMI group (p = 0.06). In conclusion, we showed beneficial effect of NAC/DFX association in improving left ventricle function in an experimental AMI.

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Section: Treatmentmentioning

confidence: 99%

Section: Treatmentmentioning

confidence: 99%

N-acetylcysteine Plus Deferoxamine Improves Cardiac Function in Wistar Rats After Non-reperfused Acute Myocardial Infarction

Phaelante

Rohde

Lopes

et al. 2015

J. of Cardiovasc. Trans. Res.

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“…29 Vietta et al reported that although cTnI levels measured 2 hours after CAL were not correlated with the SIA, those measured 8 hours after the procedure were correlated with that, but these levels could not discriminate the small, moderate or large infarct sizes from each other. 12 Additionally, it was reported in the study of Li et al that cTnI levels measured in the first four hours of CAL did not have a significant correlation with the SIA, but BNP levels had. 24 In the review of Csonka et al, it was concluded that troponin levels are not helpful in estimating the size of experimental infarction due to frequent false positive results.…”

Section: Presence Of An Infarct Area Equal To or Greater Than 20%mentioning

confidence: 94%

“…These methods are serum markers, echocardiography, magnetic resonance imaging (MRI), computed tomography, single photon emission computed tomography, and positron emission tomography. 3,7,[10][11][12] But they have disadvantages including low throughput, high costs, technical difficulties, low availability of these tests, and the need for profession and experience of performing them. 7,12 Therefore a reliable, achievable, and non-invasive alternative is highly desirable.…”

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confidence: 99%

“…3,7,[10][11][12] But they have disadvantages including low throughput, high costs, technical difficulties, low availability of these tests, and the need for profession and experience of performing them. 7,12 Therefore a reliable, achievable, and non-invasive alternative is highly desirable. 7 Following the report of Normann et al describing electrocardiographic alterations with myocardial infarction in rats, 9-lead and 12-lead electrocardiography (ECG) recordings, a cheap and easy-to-perform test, have been used to predict the SIA induced by CAL before sacrificing the animal.…”

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The Importance of ECG, Cardiac Troponin T and High Sensitive C Reactive Protein in Predicting the Extent of Experimental Myocardial Infarction in Rats

Aşgün¹,

Bağla²,

Ercan³

2017

Turkiye Klinikleri J Lab Anim

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A AB BS S T TR RA AC CT T O Ob bj je ec ct ti iv ve e: :To analyze the predictive value of ECG variables, cardiac troponin T (cTnT) and high-sensitive C reactive protein (hsCRP) levels in estimating the degree of experimental myocardial infarction in the living rats before euthanasia. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : Permanent ligation of the left anterior descending coronary artery was performed to develop acute myocardial infarction in 4-month-old male Wistar rats (n=18). Saline (n=5), low-dose erythropoietin (5 000 U.kg−1, n=6) and high-dose erythropoietin (10 000 U.kg−1, n=7) were administered intraperitoneally after the ligation to obtain varying degree of infarction. ECG records were taken before (phase 1), at the end of (phase 2), and at 6 hours after the ligation (phase 3). cTnT and hsCRP levels were measured in phase 3. The size of infarct area was calculated with planimetry on a single midventricular slice stained with triphenyltetrazolium chloride. R Re es su ul lt ts s: : The height of ST segment in phase 2, the Q/R and ST/R ratios measured in phase 3, and the Q/R ratio difference between phases 2 and 3 were found to be associated with the large infarct area. The cutoff values estimated for these variables were capable of predicting the presence of an infarct area ≥40%. cTnT and hsCRP levels were unsuccessful in estimating the size of infarction. C Co on nc cl lu us si io on n: : The variables of a 3-lead ECG may have a predictive value in estimating the presence of large infarction before euthanasia. ST segment elevation may be significantly different as early as the first-half hour of coronary ligation. The levels of cTnT and hsCRP are not associated with the size of infarction, and do not have a predictive value.K Ke ey y W Wo or rd ds s: : Myocardial infarction; erythropoietin; troponin t; c-reactive protein; electrocardiography Ö ÖZ ZE ET T A Am ma aç ç: : EKG değişkenleri, kardiyak troponin T (cTnT) ve yüksek duyarlıklı C reaktif protein (hsCRP) seviyelerinin, ratlarda oluşturulan deneysel miyokard infarktüsü derecesini ötenazi önce-sinde canlı hayvanda belirlenmesi için kullanılabilirliğinin araştırılmasıdır. G Ge er re eç ç v ve eY Yö ön nt te em ml le er r: : Dört aylık erkek Wistar ratlarda (n=18) akut miyokard infarktüsü oluşturmak için sol ön inen koroner artere kalıcı ligasyon uygulandı. Değişen büyüklüklerde infarktüs alanı elde etmek için ligasyon sonrası salin (n=5), düşük doz eritropoietin (5000 U.kg−1, n=6) ve yüksek doz eritropoietin (10000 U.kg−1, n=7) periton içine uygulandı. Ligasyon öncesinde (faz 1), ligasyon işlemi sonunda (faz 2) ve ligasyondan 6 saat sonra (faz 3) 3 elektrotlu EKG kayıtları alındı. cTnT ve hsCRP seviyeleri faz 3'de ölçüldü. İnfarktüs alanı, ventrikül ortasından alınan ve trifeniltetrazolyum klorür ile boyanan tek kesitte planimetri yöntemiyle hesaplandı. B Bu ul lg gu ul la ar r: : Faz 2'de ST segment yüksekliği, faz 3'de Q/R ve ST/R oranları ile faz 2 ve faz 3 arasında Q/R oranları farkı, büyük infarktüs alanı varlığı ile ilişkil...

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Design of affinity peptides from natural protein ligands: A study of the cardiac troponin complex

et al. 2014

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We describe a general strategy for the design and discovery of affinity peptides for a protein from its natural ligands. Our approach is guided by protein-protein interactions in natural systems and focuses on the hetero-trimeric complex of cardiac troponin I (cTnI), C (cTnC) and T (cTnT). A key premise of this work is that cTnC and cTnT, owing to their innate ability to bind cTnI, are potential templates for the design and discovery of cTnI-binding peptides. Relying only on the knowledge of primary sequences of cTnC and cTnT, we designed a library of short overlapping peptides that span the entirety of cTnC and cTnT and tested them for binding to cTnI. We were successful in identifying several peptides that display high affinity (1-100 nM) for cTnI. The specific implication of this work is that mimicking natural protein-protein interactions is an excellent starting point for the discovery and rational design of peptide ligands. The knowledge of secondary or tertiary structures of the proteins involved is not a necessary precondition for this approach. Nevertheless, we show that structural information can be used to validate the results of a fragment-based peptide design, and can be potentially beneficial for refining the lead candidates. Our approach is broadly applicable to any protein with at least one natural binding ligand with known primary sequence. For protein targets with multiple natural ligands, this approach can potentially yield several distinct affinity peptides capable of simultaneously binding the target protein via orthogonal modes or at complementary interfaces.

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Early use of cardiac troponin-I and echocardiography imaging for prediction of myocardial infarction size in Wistar rats

Abstract: A cutoff value of 4.8ng/mL for cTnI could be used as early as 8h after MI to accurately identify infarct in this model, whereas echocardiographic images taken 48h after MI predicted the infarcted area 14days after MI.

Cited by 12 publications

References 29 publications

N-acetylcysteine Plus Deferoxamine Improves Cardiac Function in Wistar Rats After Non-reperfused Acute Myocardial Infarction

N-acetylcysteine Plus Deferoxamine Improves Cardiac Function in Wistar Rats After Non-reperfused Acute Myocardial Infarction

The Importance of ECG, Cardiac Troponin T and High Sensitive C Reactive Protein in Predicting the Extent of Experimental Myocardial Infarction in Rats

Design of affinity peptides from natural protein ligands: A study of the cardiac troponin complex

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