Ecdysteroids of a culture of tissues and cells ofAjuga turkestanica

Lev, Shery; Zakirova, R. P.; Saatov, Z.; Gorovits, M. V.; Abubakirov, N. K.

doi:10.1007/bf00605197

Cited by 8 publications

(8 citation statements)

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“…Hairy root cultures of Hyoscyamus muticus biosynthesized hyoscyamine and maintained the same biosynthetic capacity for 15 years (Kim et al 2002). Callus and cell suspension cultures derived from ovaries of A. turkestanica have previously been developed (Lev et al 1990). Multiyear cultivations of cell suspension cultures of A. turkestanica were analyzed by Zakirova and Yakubova (2002) ).…”

Section: Discussionmentioning

confidence: 99%

“…Callus cultures initiated from ovaries of A. turkestanica have achieved yields of 0.12% 20E and 0.036% turkesterone on the weight of air-dried raw material (Lev et al 1990). In later callus cultivation studies, the yield of 20E was increased from 0.1% to 0.2% and turkesterone decreased from 0.032% to 0.004% when treated with an 8 mM dose of the mutagen N-nitroso-N-methylurea (Zakirova et al 2000;Zakirova and Yakubova 2002).…”

Section: Introductionmentioning

confidence: 98%

“…Phytoecdysteroids are products of terpene biosynthesis and their structures vary according to the number, the locations, and the positions of hydroxyl substituents on a steroid backbone (Báthori and Pongrácz 2005). Air-dried tissue from A. turkestanica leaves has been reported to contain 0.02% 20E and roots contain 0.045% 20E and 0.052% turkesterone (Lev et al 1990).…”

Section: Introductionmentioning

confidence: 99%

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In vitro production of metabolism-enhancing phytoecdysteroids from Ajuga turkestanica

Cheng

Yousef

Grace

et al. 2008

Plant Cell Tiss Organ Cult

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In order to develop a sustainable source of metabolism-enhancing phytoecdysteroids, cell suspension and hairy root cultures were established from shoot cultures of wild-harvested Ajuga turkestanica, a medicinal plant indigenous to Uzbekistan. Precursors of phytoecdysteroids (acetate, mevalonic acid cholesterol) or methyl jasmonate (an elicitor) were added to subculture media to increase phytoecdysteroid accumulation. In cell suspension cultures, 20-hydroxyecdysone (20E) content increased 3-or 2-fold with the addition of 125 or 250 lM methyl jasmonate, respectively, compared to unelicited cultures. Precursor addition, however, did not provoke phytoecdysteroid accumulation. In hairy root cultures, addition of sodium acetate, mevalonic acid, and methyl jasmonate, but not cholesterol, increased phytoecdysteroid content compared to unelicited cultures. Hairy root cultures treated with 150 mg l -1 sodium acetate, or 15 or 150 mg l -1 mevalonic acid, increased 20E content approximately 2-fold to 19.9, 20.4 or 21.7 lg mg -1 , respectively, compared to control (10.5 lg mg -1 ). Older hairy root cultures, extracted after the seventh subculture cycle, also showed increases in 20E content (24.8 lg mg -1 ), turkesterone (0.9 lg mg -1 ) and cyasterone (8.1 lg mg -1 ) compared to control cultures maintained for a shorter duration of four subculture cycles. Doses of 10 or 20 lg ml -1 hairy root extract increased protein synthesis by 25.7% or 31.1%, respectively, in a C2C12 mouse skeletal cell line. These results suggest that sustainable production of metabolically active phytoecdysteroid can be achieved through hairy root culture systems. Keywords 20-Hydroxyecdysone Á Cell suspension culture Á Cyasterone Á Hairy root Á Methyl jasmonate Á Mevalonic acid Á Turkesterone Abbreviations 20E 20-Hydroxyecdysone CC Column chromatography CD 3 OD Deuterated methanol DAD Diode array detector DMEM Dulbecco's modified eagle's media DPM Decays per minute DW Dry weight ESI-MS Electrospray ionization-mass spectroscopy FW Fresh weight HPLC High performance liquid chromatography Electronic supplementary material The online version of this article (

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In vitro production of metabolism-enhancing phytoecdysteroids from Ajuga turkestanica

Cheng

Yousef

Grace

et al. 2008

Plant Cell Tiss Organ Cult

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“…In 1987, Klaus Richter et al's study contacted toxicity of different solvent extracts from Ajuga reptans against Periplaneta americana. 24) Then, Lev. et al (1990) studied the possibility of obtaining ecdysteroids -ecdysterone and turkesterone-with the aid of a culture of the tissues and cells of the plant Ajuga turkestanica.…”

mentioning

confidence: 99%

“…et al (1990) studied the possibility of obtaining ecdysteroids -ecdysterone and turkesterone-with the aid of a culture of the tissues and cells of the plant Ajuga turkestanica. 25) In the post-studies, the researchers focused on the 20E production associated with the hairy roots (especially induced hairy roots) or callus cultures. [26][27][28][29] And some studies demonstrated that this plant tissue culture is an excellent experimental system for the study of "ecdysteroid biosynthesis" 30,31) and there are extensive research studies about the activities of some related enzymes.…”

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confidence: 99%

20-hydroxyecdysone accumulation and regulation in Ajuga lobata D. Don suspension culture

Qian

Yang

et al. 2016

Bioscience, Biotechnology, and Biochemistry

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Suspension culture of Ajuga lobata D. Don cells provides a method of synthesis of the phytoecdysteroid 20-hydroxyecdysone (20E) which can regulate the molting process of larvae. We characterized the culture conditions to optimize 20E production. Growth of A. lobata D. Don cells fits the logistic equation curve with a growth cycle of 19 days. Medium conductivity was negatively correlated with dry cell weight and 20E accumulation, thus could be used to determine the optimal time for cell harvest. Continuous subculture reduced 20E synthesis, but supplementing medium with 20E precursors mevalonic (MVA), α-Pinene, and nitric oxide (NO) can significantly promote cell growth and influence 20E accumulation. Combination of α-Pinene, MVA, and SNP significantly elevated 20E accumulation, thus may synergistically enhance 20E synthesis in A. lobata D. Don. The optimal concentrations of α-Pinene, MVA, and NO donor SNP in suspension culture were 50 μL L(-1), 10 mg L(-1), and 80 μmol L(-1).

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