Echinoderm Antimicrobial Peptides to Contrast Human Pathogens

Schillaci, Domenico

doi:10.4172/2329-6836.1000109

Cited by 3 publications

(2 citation statements)

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“…Sea-stars have been studied extensively in the past few years due to their diverse bioactivities and secondary metabolites (Ferguson, 1964;Schillaci & Arizza, 2013). Inhibition of fibrinogenolytic activity of SCF using zymogram protease assay.…”

Section: Discussionmentioning

confidence: 99%

Hemotoxic and wound healing potential of coelomic fluid of sea-star Astropecten indicus

Baveja¹,

Sarkar²,

Chakrabarty³

2018

JoBAZ

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Background: The coelomic fluid of echinoderms bathes all the internal organs and is a natural reservoir of various bio-active compounds. However, coelomic fluid from Indian sea-star Astropecten indicus is not well characterized for its biochemical and pharmacological profiles. Sea-star (or starfish) Astropecten indicus was collected from the coast of Goa, India during low tides. Sea-star coelomic fluid (SCF) extracted from the sea-stars was concentrated, filtered, and assayed for various hemotoxic activities such as fibrinogenolysis, fibrinolysis, hemolysis, and effects on platelet aggregation. Results: Studies on effects of SCF on A-549 human lung cancer cell line showed its non-cytotoxic and wound healing abilities in vitro. SCF was found to possess fibrinogen and fibrin digesting ability at a dose of 4 μg/ml within 30 min of incubation with the substrate. Additionally, SCF could hemolyze goat RBC by 50% and human RBC by only 5%. SDS-PAGE analysis showed that SCF contains various proteins and peptides. Two protein components of SCF yielded strong fibrinolytic, fibrinogenolytic, caseinolytic, and gelatinase activities as revealed by zymography. SCF enhanced the aggregation of ADP and collagen-activated platelets in a dose-dependent manner and had a specific proteolytic activity of 2.5 μg/ml in 30 min at 37°C as assayed using azocasein as substrate. Conclusion: This study focuses on the anticoagulant, pro-platelet aggregation and cell proliferative potential of SCF in vitro and is the first report on hemotoxic and wound healing potential of coelomic fluid of Astropecten indicus.

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Section: Discussionmentioning

confidence: 99%

Hemotoxic and wound healing potential of coelomic fluid of sea-star Astropecten indicus

Baveja¹,

Sarkar²,

Chakrabarty³

2018

JoBAZ

View full text Add to dashboard Cite

show abstract

“…Dentre os invertebrados, o filo Echinodermata agrupa organismos deuterostômios, essencialmente marinhos, que apresentam simetria pentarradial quando adultos (Pawson, 2007). Equinodermos também apresentam intrincados mecanismos de Processo inflamatório imunidade humoral (Canicatti, 1990;1991;Schillaci e Arizza, 2013), no entanto, a resposta celular é considerada a principal estratégia imunológica nestes animais (Gliński e Jarosz, 2000;Smith et al, 2006). Existem estudos tratando dos seus tipos celulares (Smith, 1981;Chia e Xing, 1996;Smith et al, 2006;.…”

Section: Processo Inflamatório Introduçãounclassified