The object of this study was the recognition of Regions Of Interest(ROIs) in a time series of digital images of two specific laboratory experiments. It concerns the identification of objects in a tissue surface by high-resolution and high-speed ad-hoc systems for morphological dynamic image analysis. The protocols and the algorithms implemented are developed to retrieve biomechanical properties of two different in vitro systems; the solid filament X-MET (eX-vivo Muscle Engineered Tissue) to measure its reaction to a different frequency stimulation, and a planar system of co-culture of skeletal and cardiac muscle cells, where myotubes and cardiomyocytes coexist, to discriminate the interaction between different cell's type, of its spontaneous pulse. The results of the stimulated X-MET from solid culture system are frequency dependent points of the macroscopic muscular strength and its contractile response. The results for the co-culture planar board measure the correlation of the pulsed movements of the different parts of the tissue.