ECM-mimetic immunomodulatory hydrogel for methicillin-resistant Staphylococcus aureus –infected chronic skin wound healing

Abstract

The treatment of difficult-to-heal wounds remains a substantial clinical challenge due to deteriorative tissue microenvironment including the loss of extracellular matrix (ECM), excessive inflammation, impaired angiogenesis, and bacterial infection. Inspired by the chemical components, fibrous structure, and biological function of natural ECM, antibacterial and tissue environment–responsive glycopeptide hybrid hydrogel was developed for chronic wound healing. The hydrogel can facilitate the cell proliferation and macrophage polarization to M2 phenotype, and show potent antibacterial efficacy against both Gram-negative and Gram-positive bacteria. Significantly, the glycopeptide hydrogel accelerated the reconstruction of methicillin-resistant Staphylococcus aureus (MRSA)–infected full-thickness diabetic and scalding skin by orchestrating a pro-regenerative response indicated by abundant M2-type macrophages, attenuated inflammation, and promoted angiogenesis. Collectively, ECM-mimetic and immunomodulatory glycopeptide hydrogel is a promising multifunctional dressing to reshape the damaged tissue environment without additional drugs, exogenous cytokines, or cells, providing an effective strategy for the repair and regeneration of chronic cutaneous wounds.

Effect of composite biodegradable biomaterials on wound healing in diabetes

“…The collagen sponge with anti-infective bioactive molecules also effectively restored the normal function of fibroblasts and keratinocytes in infectious wounds (Ramanathan et al, 2017). The latest research shows that glycopeptide hydrogel accelerates the reconstruction of fullthickness diabetes and scalded skin infected by methicillinresistant Staphylococcus aureus (MRSA) by coordinating a large number of M2-type macrophages, reducing inflammation and promoting angiogenesis (Liu W. et al, 2022). We summarize the evolution of wound dressing function through a set of figures (Figure 2).…”

“…In addition, the presence of a large number of proteases in the microenvironment of diabetes Frontiers in Bioengineering and Biotechnology frontiersin.org wounds leads to the degradation of GFS, which further hinders angiogenesis and diabetes wound healing. Some studies have shown that ECM simulation and immune regulation can be achieved through the modification of the material itself (Liu W. et al, 2022), to achieve the antibacterial effect. However, the healing of diabetes wounds does not depend solely on the antibacterial effect.…”

Reversible Regulation of the Reactive Oxygen Species Level Using a Semiconductor Heterojunction

“…The treatment of infected wounds not only required an upfront antibacterial and anti-infective strategy but also needed the suppression of chronic inflammation during the healing process. Many effective approaches have been proposed for bacterial inhibition and promoting infected wound healing. , For example, metal nanoparticles could defend against bacteria through the release of metal ions, photothermal and photocatalytic ability, immunomodulatory hydrogel , that orchestrated a pro-regenerative response by abundant macrophages, and attenuated inflammation. Ce/Cu-DBs possessed a heterojunction structure composed of two nanozymes.…”

“…Many effective approaches have been proposed for bacterial inhibition and promoting infected wound healing. 36,37 For example, metal nanoparticles 38 could defend against bacteria through the release of metal ions, photothermal and photocatalytic ability, immunomodulatory hydrogel 39,40 that orchestrated a pro-regenerative response by abundant macrophages, and attenuated inflammation. Ce/Cu- in the efficiency of ROS production and scavenging in the pH 5−7 range.…”

A Heterocatalytic Metal–Organic Framework to Stimulate Dispersal and Macrophage Combat with Infectious Biofilms

“…Both approaches neglected the consideration of potential septic symptoms, while the latter approach necessitated near-infrared irradiation, which provides a severe drawback compared to the use of our Pt-NP/UiO-66 MOFs, requiring no additional light triggering. Other approaches have advocated a preference for stimulating polarization toward the M2-phenotype to facilitate angiogenesis and blood vessel maturation in order to accelerate healing of the skin after chronic wound infection . Whereas this may be preferable in order to stimulate tissue healing, bacterial source elimination, particularly of pathogens involved in life-threatening sepsis, may be considered a first priority from a clinical perspective.…”