Eco-Efficient Quantification of Glucosinolates in Camelina Seed, Oil, and Defatted Meal: Optimization, Development, and Validation of a UPLC-DAD Method

Abstract

Camelina sativa (camelina) seed, oil, and defatted meal are widely used for food, animal feed, and other purposes. The accurate quantification of camelina glucosinolates is critical as their functionalities are highly dose-dependent. The classic quantification of glucosinolates in camelina products involves tedious desulfation steps, toxic reagents, and a lengthy instrument time because glucosinolates are easy to degrade and subject to interference in the liquid chromatography. Thus, we developed and validated an eco-efficient UPLC-DAD method for determining glucoarabin (GS9), glucocamelinin (GS10), and homoglucocamelinin (GS11) in camelina seed, oil, and defatted meal. Glucosinolates were extracted using 80% cold methanol to denature myrosinase, and were separated by an HSS T3 column without desulfation. Glucotropaeolin was used as an internal standard to track analyte degradation and loss during sample preparation. The method has shown high precision (relative standard deviations ranging from 4.12% to 6.54%) and accuracy (>94.4% spike recovery) for GS9-11, and all validation parameters passed the industry-consensus AOAC Appendix F criteria. To our best knowledge, this is the first eco-efficient and low-cost analytical method that is validated against strict AOAC criteria for the quantification of intact camelina glucosinolates. The method is suitable to be adopted as a new industrial testing standard to assist in the quality control of camelina products.

Effect of different doses of camelina cake inclusion as a substitute of dietary soyabean meal on growth performance and gut health of weaned pigs

“…Camelina seeds are characterised by the presence of aliphatic glucosinolates: 9-(methylsulfinyl)nonyl glucosinolate (also known as glucoarabin or GS9), 10-(methylsulfinyl)decyl glucosinolate (also known as glucocamelinin or GS10) and 11-(methylsulfinyl)undecyl glucosinolate (also known as homoglucocamelinin or GS11) (41,42) . Depending on their nature, glucosinolates can either be hydrolysed by myrosinases which can be produced by gut microorganisms (43) producing progoitrin which is spontaneously converted into goitrin at neutral pH and into nitriles at a more acidic pH (< 5·5) (44) , or they can be degraded into isothiocyanates and then to indole-3-carbinol by the release of thiocyanate at neutral pH, and to indole-3-acetonitrile at acidic pH (4–5·6) (45,46) .…”

Phytochemical drivers of insect herbivory: a functional toolbox to support agroecological diversification

“…In general, cold temperature limits spurious changes in metabolites owing to biochemical reactions or thermal lability that could affect the integrity of metabolomic profiles [49,50]. In the specific case of glucosinolates, some studies confirmed that inorganic solvents maintained at cold temperatures prevent their degradation by the myrosinase enzyme [51][52][53]. In brief, shoots were crushed in mortar filled with liquid nitrogen and 215.35 ± 10.38 mg of fresh mass was transferred to a 2 ml Eppendorf tube.…”

Boosting Salt Tolerance in Camelina cultivars through synergistic Impact of Exogenous Silicon and Selenium by modulating physiological attributes and Antioxidant activities

“…The unique fatty acid composition of camelina oil has favorable nutritional and physiological effects, such as reducing blood triglyceride and cholesterol levels. As a result, camelina oil is considered suitable for direct consumption, cooking, and as an enrichment ingredient in products such as margarine, salad dressing, mayonnaise, and ice cream, providing omega-3 fatty acids (Meza et al 2022;Li et al 2023).…”