Eco-friendly approach to control dengue vector Aedes aegypti larvae with their enzyme modulation by Lumnitzera racemosa fabricated zinc oxide nanorods

Abstract

Aedes aegypti mosquito species is a primary vector for dengue, chikungunya and Zika infections, and vector control is the only key approach for reducing their transmission. The present study emphasizes on environmental friendly approach for the fabrication of zinc oxide nanorods (ZnO NRs) using aqueous extract of Lumnitzera racemosa flower buds (LB) as a reducing and stabilizing agent. ZnO NRs were examined by UV-Vis spectroscopy with characteristic absorbance band at 373.82 nm and bandgap of 3.25 eV. FT-IR analysis revealed the functional groups associated with ZnO NRs. The crystallinity of LB-ZnO NRs was further investigated using X-ray diffraction. The Zeta potential displayed a surface charge of − 23.5 mV on NRs. Energy dispersive spectra analysis and SEM analysis confirmed the formation of ZnO NRs whilst TEM highlighted the average length and diameter in the range of 250-300 nm and 45-50 nm respectively. LB-ZnO NRs were found to be efficacious against Ae. aegypti 4th instar larvae with LC 50 of 24.74 μg/ml. Decreased acetylcholinesterase (60.6%) and glutathione S-transferase (24.4%) activities were also evaluated in Ae. aegypti larvae which were exposed to synthesized LB-ZnO NRs with no genetic aberrations. All these outcomes propose the potential employment of LB-ZnO NRs in mosquito control, as well as an effective alternative to insecticide resistance.

Effects of Puncture Vine Plant (Tribulus Terrestris L.) Extract on All Larval Stages of Mosquito, Culex Pipiens (Diptera: Culicidae)

“…The present study also revealed obvious morphological abnormalities, in addition to morphological abnormalities . The results also demonstrated that larval mortality was time-related, and the results were consistent with those of Dhavan and Jadhav(2020).…”

Ultrasound‐Assisted Green Synthesis of Ag‐Decorated ZnO Nanoparticles UsingExcoecaria agallochaLeaf Extract and Evaluation of Their Photocatalytic and Biological Activity

“…ZnO is direct band gap semiconductor with the band gap 3.37 eV. Thus, tauc‘s equation used to calculate the band gap [47] …”

“…Thus, tauc's equation used to calculate the band gap. [47] ðahuÞ ¼ Aðhu-E g Þ n here, n is 1 = 2 for direct transition semiconductor A is constant and α is adsorption coefficient. To calculate the band gap, absorption plot was converted to tauc's plot [(αhυ) 2 vshυ] using absorption onset.…”

Novel Leea grandifolia leaves mediated synthesis of ZnO nanorods for photocatalytic and anticancer applications

“…[ 14,27 ] Table 1 shows the various plant‐mediated synthesis zinc oxide NPs with various size and their biological applications. [ 34–40 ] The L. grandifolia plant leaf‐mediated synthesized zinc oxide NRs reveals less aggregated structure and lower size 20 to 200 nm, compared to other zinc oxide nanoparticles.…”