Eco-Friendly Formulated Zinc Oxide Nanoparticles: Induction of Cell Cycle Arrest and Apoptosis in the MCF-7 Cancer Cell Line

Moghaddam, Amin Boroumand; Moniri, Mona; Azizi, Susan; Rahim, Raha Abdul; Ariff, Arbakariya; Navaderi, Mohammad; Mohamad, Rosfarizan

doi:10.3390/genes8100281

Cited by 136 publications

(59 citation statements)

References 62 publications

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“…ZnO nanostructures can induce cytotoxic and genotoxic effects to normal mammalian cells [132][133][134][135][136][137][138][139][140][141][142][143][144][145][146] and cancerous cells [50,54,[147][148][149][150][151][152][153][154][155][156][157][158][159][160][161][162]. For normal human bronchial epithelial (BEAS-2B) cells, cellular uptake of ZnO NPs leads to their dissolution into intracellular Zn 2+ ions [135].…”

Section: Normal Cellsmentioning

confidence: 99%

“…Moghaddam et al treated MCF-7 cells with biosynthesized ZnO NPs (10-59 nm) at doses of 62.5, 125, 250, 500, and 1000 µ g/mL. ZnO NPs arrested the cell cycle in the G2/M phase, upregulated pro-apoptotic genes p53, p21, Bax, and JNK, and downregulated antiapoptotic genes Bcl2, AKT1, and ERK1/2 in a dose-dependent manner [152]. Akhtar et al studied the cytotoxicity of ZnO NPs (21.34 nm) prepared by the co-precipitation process against HepG2 and A549 cancer cells as well as normal primary rat cells (astrocytes and hepatocytes) [148].…”

Section: Normal Cellsmentioning

confidence: 99%

“…Reproduced from[143] under the Creative Commons Attribution.4.1.2. Cancerous CellsNano-ZnO materials have been reported to attack multiple cancer cell lines based on cytotoxic assay results[50,126,[147][148][149][150][151][152][153][154][155][156][157][158][159][160][161][162]. Very recently, Hussain et al synthesized spherical ZnO NPs (90 nm) from Pandanus odorifer leaf extract and zinc acetate dihydrate.…”

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confidence: 99%

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Interactions of Zinc Oxide Nanostructures with Mammalian Cells: Cytotoxicity and Photocatalytic Toxicity

Liao

Jin

et al. 2020

IJMS

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This article presents a state-of-the-art review and analysis of literature studies on the morphological structure, fabrication, cytotoxicity, and photocatalytic toxicity of zinc oxide nanostructures (nZnO) of mammalian cells. nZnO with different morphologies, e.g., quantum dots, nanoparticles, nanorods, and nanotetrapods are toxic to a wide variety of mammalian cell lines due to in vitro cell–material interactions. Several mechanisms responsible for in vitro cytotoxicity have been proposed. These include the penetration of nZnO into the cytoplasm, generating reactive oxygen species (ROS) that degrade mitochondrial function, induce endoplasmic reticulum stress, and damage deoxyribonucleic acid (DNA), lipid, and protein molecules. Otherwise, nZnO dissolve extracellularly into zinc ions and the subsequent diffusion of ions into the cytoplasm can create ROS. Furthermore, internalization of nZnO and localization in acidic lysosomes result in their dissolution into zinc ions, producing ROS too in cytoplasm. These ROS-mediated responses induce caspase-dependent apoptosis via the activation of B-cell lymphoma 2 (Bcl2), Bcl2-associated X protein (Bax), CCAAT/enhancer-binding protein homologous protein (chop), and phosphoprotein p53 gene expressions. In vivo studies on a mouse model reveal the adverse impacts of nZnO on internal organs through different administration routes. The administration of ZnO nanoparticles into mice via intraperitoneal instillation and intravenous injection facilitates their accumulation in target organs, such as the liver, spleen, and lung. ZnO is a semiconductor with a large bandgap showing photocatalytic behavior under ultraviolet (UV) light irradiation. As such, photogenerated electron–hole pairs react with adsorbed oxygen and water molecules to produce ROS. So, the ROS-mediated selective killing for human tumor cells is beneficial for cancer treatment in photodynamic therapy. The photoinduced effects of noble metal doped nZnO for creating ROS under UV and visible light for killing cancer cells are also addressed.

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Section: Normal Cellsmentioning

confidence: 99%

Section: Normal Cellsmentioning

confidence: 99%

mentioning

confidence: 99%

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Interactions of Zinc Oxide Nanostructures with Mammalian Cells: Cytotoxicity and Photocatalytic Toxicity

Liao

Jin

et al. 2020

IJMS

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“…Similarly, studies also show that ZnO nanoparticles could restrain cell growth through induction of cell cycle arrest and apoptosis in multiple cancer cells. 22,23 Moreover, we further evaluated the T24 cell invasion and migration after nZnO exposure for 48 hrs. The transwell assay shows that cells treated with nZnO were lower in capacity to penetrate the membrane than those without nZnO treatment, especially for 10μg/mL nZnO ( Figure 3C).…”

Section: Resultsmentioning

confidence: 99%

<p>Anticancer Effects of Zinc Oxide Nanoparticles Through Altering the Methylation Status of Histone on Bladder Cancer Cells</p>

Zhang

Liu

et al. 2020

IJN

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Purpose: Zinc oxide nanoparticles (nZnO) have been widely used in the medicine field. Numerous mechanistic studies for nZnO's anticancer effects are merely performed under high concentration exposure. However, possible anticancer mechanisms of epigenetic dysregulation induced by low doses of nZnO are unclear. Methods: nZnO were characterized and bladder cancer T24 cells were treated with nZnO for 48 hrs at different exposure concentrations. Cell cycle, apoptosis, cell migration and invasion were determined. We performed qRT-PCR, Western blot and chromatin immunoprecipitation to detect the mRNA and protein levels of signaling pathway cascades for histone modification. Results: In this study, we investigated the potential anticancer effects and mechanisms of nZnO on histone modifications in bladder cancer T24 cells upon low-dose exposure. Our findings showed that low concentrations of nZnO resulted in cell cycle arrest at S phase, facilitated cellular late apoptosis, repressed cell invasion and migration after 48 hrs exposure. These anticancer effects could be attributed to increased RUNX3 levels resulting from reduced H3K27me 3 occupancy on the RUNX3 promoter, as well as decreased contents of histone methyltransferase EZH2 and the trimethylation of histone H3K27. Our findings reveal that nZnO are able to enter into the cytoplasm and nucleus of T24 cells. Additionally, both particles and ions from nZnO may jointly contribute to the alteration of histone methylation. Moreover, sublethal nZnO-conducted anticancer effects and epigenetic mechanisms were not associated with oxidative stress or DNA damage. Conclusion: We reveal a novel epigenetic mechanism for anticancer effects of nZnO in bladder cancer cells under low-dose exposure. This study will provide experimental basis for the toxicology and cancer therapy of nanomaterials.

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“…To overcome these limitations, an alternate way of therapies and drugs is essential to treat the cancer. Nowadays, eco-friendly based nanoparticles are attempted by the researchers as an alternate to control the cancerous cell growth [10]. Nanomaterials are expected hopefully to revolutionize cancer diagnosis and therapy [11].…”

Section: Introductionmentioning

confidence: 99%

IN VITRO GROWTH INHIBITORY EFFECT OF ZnO NANOPARTICLES ON HUMAN LIVER CANCER CELL LINES (Huh7)

Ananthalakshmi¹,

Rajarathinam²,

A³

2019

Asian J Pharm Clin Res

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Objective: The objective of the study was to access the anticancer activity of the biosynthesized ZnO nanoparticles against Huh7 liver cancer cell lines. Methods: The study was carried in vitro using Huh7 cell lines. The ZnO nanoparticles (ZnO NPs) were synthesized using Luffa acutangula peel extract and subjected to characterization by X-ray powder diffraction and transmission electron microscopy. The Huh7 cell lines were treated with ZnO NPs and done 3-(4, 5-dimethylthiazol- 2-yl)-2, 5-diphenyltetrazolium bromide assay. For live and dead assay, the cell lines treated with ZnO NPs were subjected to acridine orange/ethidium (AO/ET) bromide assay. Results: The ZnO NPs synthesized show spherical structure with 10–20 nm size. The 50% of Huh7 proliferation were inhibited at the concentration (IC50) of 40 μg/ml. The AO/ET assay shows compact nucleus and fine cytoplasmic morphology in control cells and apoptotic stage in treated cells Conclusion: This study suggests that ZnO NPs can be prepared in environment-friendly method using aqueous extract of L. acutangula and can be used in cancer treatment effectively.

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Eco-Friendly Formulated Zinc Oxide Nanoparticles: Induction of Cell Cycle Arrest and Apoptosis in the MCF-7 Cancer Cell Line

Cited by 136 publications

References 62 publications

Interactions of Zinc Oxide Nanostructures with Mammalian Cells: Cytotoxicity and Photocatalytic Toxicity

Interactions of Zinc Oxide Nanostructures with Mammalian Cells: Cytotoxicity and Photocatalytic Toxicity

<p>Anticancer Effects of Zinc Oxide Nanoparticles Through Altering the Methylation Status of Histone on Bladder Cancer Cells</p>

IN VITRO GROWTH INHIBITORY EFFECT OF ZnO NANOPARTICLES ON HUMAN LIVER CANCER CELL LINES (Huh7)

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