Eco-friendly wood-biofungicidal and antibacterial activities of various Coccoloba uvifera L. leaf extracts: HPLC analysis of phenolic and flavonoid compounds

Ashmawy, Nader A.; Salem, Mohamed Z. M.; Shanhorey, Nader El; Al-Huqail, Asma A.; Ali, Hayssam M.; Behiry, Said I.

doi:10.15376/biores.15.2.4165-4187

Cited by 27 publications

(8 citation statements)

References 64 publications

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“…The antifungal effect of the P. lagopus extract in vitro against R. solani in this study agreed with our recent research results. The ethanolic Coccoloba uvifera extract inhibited the development of R. solani , Botrytis cinerea , and Fusarium culmorum by 64.4, 100, and 38.5%, respectively ( Ashmawy et al, 2020b ). Also, the results are consistent with those documented by different authors ( Deena and Thoppil, 2000 ; Valarini et al, 2003 ).…”

Section: Discussionmentioning

confidence: 99%

Plantago lagopus extract as a green fungicide induces systemic resistance against Rhizoctonia root rot disease in tomato plants

et al. 2022

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Extensive use of chemical control agents and fungicides typically leads to numerous risks to human health and the environment. Using plant extracts as natural substances represents a dual key for the environment and sustainable food production, as it reduces the input of synthetic pesticides into the environment and/or controls plant pathogens. For the first time, a Plantago lagopus ethanolic extract has been characterized and evaluated for its protective and curative effects against Rhizoctonia solani in tomato plants. The results showed that P. lagopus extract (10 μg/ml) completely inhibited R. solani mycelial growth in vitro. At 20 days of post fungal inoculation, the results demonstrated that using P. lagopus extract (100 μg/ml) in vivo enhanced tomato plant growth by significantly increasing shoot and root parameters in protective and curative treatments. Furthermore, the protective and curative treatments significantly reduced the disease index by 18.66 and 38.66%, respectively. Induction of systemic resistance with upregulation of PR-1 and PR-2 and a significant increase in the transcriptional levels of PR-3 and CHS in all P. lagopus extract-treated tomato plants were reported compared to untreated plants. HPLC analysis showed that the most common polyphenolic components detected in P. lagopus extract were rutin (74206.3 mg/kg), naringenin (2388.74 mg/kg), quercetin (1249.13 mg/kg), and p-hydroxybenzoic acid (1035.87 mg/kg). In addition, the ellagic acid (798.47 mg/kg), vanillic acid (752.55 mg/kg), catechol (648.89 mg/kg), cinnamic acid (332.51 mg/kg), ferulic acid (296.32 mg/kg), benzoic acid (295.95 mg/kg), and chlorogenic acid (116.63 mg/kg) were also reported. Our study is the first to show that P. lagopus extract can help plants fight off R. solani fungal infection. Furthermore, the findings imply that using the P. lagopus extract as a natural biocontrol agent could be a sustainable strategy to manage plant fungal diseases.

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Section: Discussionmentioning

confidence: 99%

Plantago lagopus extract as a green fungicide induces systemic resistance against Rhizoctonia root rot disease in tomato plants

et al. 2022

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“…The uses of plant extracts and microbial bioagents as eco-friendly treatments for the management of plant diseases have recorded high significance in recent years [43,[63][64][65][66][67][68]. The literature supports the implementation of biostimulant and biocontrol tools due to nature, antimicrobial activity, easy biodegradability, non-phytotoxicity, and resistance in the host.…”

Section: Discussionmentioning

confidence: 99%

Natural Plant Extracts and Microbial Antagonists to Control Fungal Pathogens and Improve the Productivity of Zucchini (Cucurbita pepo L.) In Vitro and in Greenhouse

Hassan

Mohamed²,

Feleafel

et al. 2021

Horticulturae

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Background: Natural plant extracts and microbial antagonists have the potential for use in increasing the fungal resistance and productivity of horticulture plants. Methods: The purpose of this study was to evaluate the ability of both natural plant extracts and microbial antagonists as a biotical control of some fungal pathogens, i.e., Fusarium ssp., Exserohilum ssp. and Nigrospora ssp., along with improving the growth and productivity performance of zucchini under greenhouse conditions. Eucalyptus camaldulensis leaf extract (LE), Citrus sinensis LE, Ficus benghalensis fruit extract (FE), and two microbial antagonists Pseudomonas fluorescens (accession no. MW647093) and Trichoderma viride (accession no. MW647090) were tested under in vitro and in vivo conditions. Through morphological characteristics and the internal transcribed spacer (ITS) region, Fusarium solani (accession no. MW947256), F. oxysporum (accession no. MW947254), Exserohilum rostratum (accession no. MW947255), and Nigrospora lacticolonia (accession no. MW947253) were identified. HPLC analysis was used for the identification of phenolic compounds (PCs) and flavonoid compounds (FCs) in the extracts. Results: The highest inhibition percentage of fungal growth (IPFG) against F. oxysporum was obtained with P. fluorescens, T. viride, and E. camaldulensis LE (4000 mg/L); F. solani with P. fluorescens, T. viride, and C. sinensis LE (4000 mg/L); Exserohilum rostratum with P. fluorescens, Ficus benghalensis FE (4000 mg/L) and E. camaldulensis LE (4000 mg/L), and N. lacticolonia with P. fluorescens. Using HPLC analysis, the abundant PCs in E. camaldulensis LE were pyrogallol, and caffeic acid, those in C. sinensis LE were syringic acid and ferulic acid, and those in F. benghalensis FE were gallic acid and syringic acid. In addition, the abundant FCs in E. camaldulensis LE were kaempferol, and naringin, those in C. sinensis LE were hesperidin and quercetin, and those in F. benghalensis FE were kaempferol and quercetin. Under greenhouse experiments, T. viride and E. camaldulensis LE (4000 mg/L) followed by P. fluorescens + T. viride treatments gave the best results of zucchini plants in terms of leaf area, fruits number per plant, yield per plant, and total yield (marketable and non-marketable). Conclusions: Plant extracts and bioagents can be used to control some zucchini fungal pathogens and increase the productivity performance of zucchini plants.

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“…As reported before, different methods, including chemical fungicides and biological control agents, work well to minimize crop loss resulting from fungal infections [36,37]. In our previous study, the growth of Fusarium culmorum, Rhizoctonia solani, and Botrytis cinerea was suppressed by 38.5%, 64.4%, and 100%, respectively, when using the ethanol extract of Coccoloba uvifera at 30 µg/mL [38]. R. solani and F. culmorum growth were In our previous study, the growth of Fusarium culmorum, Rhizoctonia solani, and Botrytis cinerea was suppressed by 38.5%, 64.4%, and 100%, respectively, when using the ethanol extract of Coccoloba uvifera at 30 µg/mL [38].…”

Section: Speciosa Extract Inhibitory Effect In Vitromentioning

confidence: 64%

“…In our previous study, the growth of Fusarium culmorum, Rhizoctonia solani, and Botrytis cinerea was suppressed by 38.5%, 64.4%, and 100%, respectively, when using the ethanol extract of Coccoloba uvifera at 30 µg/mL [38]. R. solani and F. culmorum growth were In our previous study, the growth of Fusarium culmorum, Rhizoctonia solani, and Botrytis cinerea was suppressed by 38.5%, 64.4%, and 100%, respectively, when using the ethanol extract of Coccoloba uvifera at 30 µg/mL [38]. R. solani and F. culmorum growth were highly inhibited at a 30 µg/mL concentration of an n-hexane Eucalyptus camaldulensis extract [39].…”

Section: Speciosa Extract Inhibitory Effect In Vitromentioning

confidence: 91%

Chorisia speciosa Extract Induces Systemic Resistance against Tomato Root Rot Disease Caused by Rhizoctonia solani

Behiry

Soliman²,

Al-Mansori³

et al. 2022

Agronomy

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Chemical pesticides and fungicides are used extensively, negatively affecting people’s health and the environment. Reducing synthetic pesticides and increasing the efficiency of sustainable food production using plant extracts as natural chemicals is a win–win. Here, we first describe and evaluate an ethanolic extract of Chorisia speciosa for its protective and curative activities against Rhizoctonia solani in greenhouse-grown tomato plants. The results showed that the mycelial growth of R. solani was completely suppressed in vitro by C. speciosa extract (10 µg/mL). Twenty days after fungal inoculation, the results demonstrated that using C. speciosa extract (10 µg/mL) in vivo significantly improved shoot and root growth parameters in protective and curative treatments. Further, the protective and curative treatments decreased the disease index by 26.67% and 53.33%, respectively. C. speciosa-treated tomato plants showed significantly increased antioxidant enzyme production (PPO, CAT, and SOD) and up-regulated PR-1, PR-2, PR-3, PAL, and CHS expression levels compared to untreated plants. According to HPLC examination, the most prevalent phenolic acids or flavonoid components quantities (µg/mL) noticed in C. speciosa extract were 7-OH-flavone (10.36), kaempferol (9.23), p-coumaric acid (8.65), ferulic acid (8.14), caffeic acid (7.59), gallic acid (6.33), and iso-ferulic (5.71). Our findings are the first to demonstrate that a C. speciosa extract can assist plants in combating fungal infestation. Therefore, the data imply that C. speciosa extract, as a natural and renewable product, could be adopted as a long-term approach for regulating plant fungus.

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Eco-friendly wood-biofungicidal and antibacterial activities of various Coccoloba uvifera L. leaf extracts: HPLC analysis of phenolic and flavonoid compounds

Cited by 27 publications

References 64 publications

Plantago lagopus extract as a green fungicide induces systemic resistance against Rhizoctonia root rot disease in tomato plants

Plantago lagopus extract as a green fungicide induces systemic resistance against Rhizoctonia root rot disease in tomato plants

Natural Plant Extracts and Microbial Antagonists to Control Fungal Pathogens and Improve the Productivity of Zucchini (Cucurbita pepo L.) In Vitro and in Greenhouse

Chorisia speciosa Extract Induces Systemic Resistance against Tomato Root Rot Disease Caused by Rhizoctonia solani

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