Eco-, geno- and human toxicology of bio-active nanoparticles for biomedical applications

Robbens, Johan; Vanparys, Caroline; Nobels, Ingrid; Blust, Ronny; Hoecke, Karen Van; Janssen, Colin R.; Schamphelaere, Karel A.C. De; Roland, Kathleen; Blanchard, Gersande; Silvestre, Frédéric; Gillardin, Virginie; Kestemont, Patrick; Anthonissen, Roel; Toussaint, Olivier; Vankoningsloo, Sébastien; Saout, Christelle; Alfaro-Moreno, Ernesto; Hoet, Peter; Gonzalez, Laetitia; Dubruel, Peter; Troisfontaines, Paul

doi:10.1016/j.tox.2009.11.002

Cited by 45 publications

(28 citation statements)

References 25 publications

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“…They are a direct extension of methods known for other macroscopic biomaterials or soluble drug toxicology assessments. However, since there are no defined protocols for assessing nanotoxicity [15,16], the use of the current assays to evaluate the toxic potential and the mechanisms involved is of great importance in determining structure/function relationships between nanomaterials and toxicity.…”

Section: Resultsmentioning

confidence: 99%

“…Currently, there are no specific testing requirements for nanotechnology products and, therefore, researchers took liberal approaches to studying toxicity [15,16]. Moreover, it is worth noting that, because of the expense of animal testing in toxicology and pressure from both the general public and government to develop alternatives to in vivo testing, in vitro cellbased models may be more attractive for preliminary testing of nanomaterials [17].…”

Section: Introductionmentioning

confidence: 99%

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New cationic nanovesicular systems containing lysine-based surfactants for topical administration: Toxicity assessment using representative skin cell lines

Nogueira

Morán

Mitjans

et al. 2013

European Journal of Pharmaceutics and Biopharmaceutics

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Cationic nanovesicles have attracted considerable interest as effective carriers to improve the delivery of biologically active molecules into and through the skin. In this study, lipid-based nanovesicles containing three different cationic lysine-based surfactants were designed for topical administration. We used representative skin cell lines and in vitro assays to assess whether the cationic compounds modulate the toxic responses of these nanocarriers.The nanovesicles were characterized in both water and cell culture medium. In general, significant agglomeration occurred after 24 h incubation under cell culture conditions. We found different cytotoxic responses among the formulations, which depended on the surfactant, cell line (3T3, HaCaT and THP-1) and endpoint assayed (MTT, NRU and LDH).Moreover, no potential phototoxicity was detected in fibroblast or keratinocyte cells, whereas only a slight inflammatory response was induced, as detected by IL-1α and IL-8 production inHaCaT and THP-1 cell lines, respectively. A key finding of our research was that the cationic charge position and the alkyl chain length of the surfactants determine the nanovesicles resulting toxicity. The charge on the α-amino group of lysine increased the depletion of cell metabolic activity, as determined by the MTT assay, while a higher hydrophobicity tends to enhance the toxic responses of the nanovesicles. The insights provided here using different cell lines and assays offer a comprehensive toxicological evaluation of this group of new nanomaterials.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

New cationic nanovesicular systems containing lysine-based surfactants for topical administration: Toxicity assessment using representative skin cell lines

Nogueira

Morán

Mitjans

et al. 2013

European Journal of Pharmaceutics and Biopharmaceutics

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“…Gold and silver nanoparticles of triangular morphology are particularly interesting for investigation because of their potential biomedical applications [35,36]. The synthesis of nanoparticles using chemical methods (Turkevich recipe) has been reported and used routinely, but often these chemically synthesized gold nanotriangles are less stable, in addition to the toxic byproducts of these reactions that create environmental hazards and potential risk to the human lives in several means [37]. Thus, microbe-mediated synthesis of nanomaterials of desired shape should be a good alternative to the chemical method that can effectively reduce environmental toxicity.…”

Section: Introductionmentioning

confidence: 99%

Biogenic gold nanotriangles from Saccharomonospora sp., an endophytic actinomycetes of Azadirachta indica A. Juss.

et al. 2013

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Microbial biofabrication is emerging as eco-friendly, simpler, and reproducible alternative to chemical synthesis of metals and semiconductor nanoparticles, allowing generation of rare geometrical forms such as nanotriangles and nanoprisms. Highly confined nanostructures like triangles/prisms are interesting class of nanoparticles due to their unique optical properties exploitable in biomedical diagnostics and biosensors. Here, we report for the first time a single-step biological protocol for the synthesis of gold nanotriangles using extract of endophytic actinomycetes Saccharomonospora sp., isolated from surface sterilized root tissues of Azadirachta indica A. Juss., when incubated with an aqueous solution of chloroaurate ions (AuCl − 4 /1 mM). Thin, flat occasionally prismatic gold nanotriangles were produced when aqueous chloroaurate ions reacted with the cell-free extract as well as with the biomass of endophytic Saccharomonospora. It was evidenced from sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis that proteins of 42 and 50 kD were involved in biosynthesis as well as in stabilization of the nanoparticles. The particle growth process was monitored by UV-vis spectroscopy, and the morphological characterization was carried out by transmission electron microscopy and atomic force microscopy together with X-ray powder diffractions. Although the exact mechanism for this shape-oriented synthesis is not clear so far, the possibility of achieving nanoparticle shape control in a microbial system is exciting.

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“…In that report, no adverse effect was observed. 26 However, since the genotoxic potential of nanoparticles is strongly dependent on their surface properties, 27 the role of nanoparticle surface chemistry and surface charge on their in vitro toxicity needed to be investigated thoroughly. In the present work, we have designed three types of surface-modified nanoparticles, ie, positively and negatively charged as well as neutral, and performed a direct comparison of the various surface coatings.…”

Section: Introductionmentioning

confidence: 99%

Influence of surface charge on the potential toxicity of PLGA nanoparticles towards Calu-3 cells

Mura¹,

Hillaireau²,

Nicolas³

et al. 2011

IJN

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Background Because of the described hazards related to inhalation of manufactured nanoparticles, we investigated the lung toxicity of biodegradable poly (lactide-co-glycolide) (PLGA) nanoparticles displaying various surface properties on human bronchial Calu-3 cells. Methods Positively and negatively charged as well as neutral nanoparticles were tailored by coating their surface with chitosan, Poloxamer, or poly (vinyl alcohol), respectively. Nanoparticles were characterized in terms of size, zeta potential, and surface chemical composition, confirming modifications provided by hydrophilic polymers. Results Although nanoparticle internalization by lung cells was clearly demonstrated, the cytotoxicity of the nanoparticles was very limited, with an absence of inflammatory response, regardless of the surface properties of the PLGA nanoparticles. Conclusion These in vitro results highlight the safety of biodegradable PLGA nanoparticles in the bronchial epithelium and provide initial data on their potential effects and the risks associated with their use as nanomedicines.

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Eco-, geno- and human toxicology of bio-active nanoparticles for biomedical applications

Cited by 45 publications

References 25 publications

New cationic nanovesicular systems containing lysine-based surfactants for topical administration: Toxicity assessment using representative skin cell lines

New cationic nanovesicular systems containing lysine-based surfactants for topical administration: Toxicity assessment using representative skin cell lines

Biogenic gold nanotriangles from Saccharomonospora sp., an endophytic actinomycetes of Azadirachta indica A. Juss.

Influence of surface charge on the potential toxicity of PLGA nanoparticles towards Calu-3 cells

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