The anthropogenic factor has a particularly acute effect on natural resources. The state of biodiversity of biotopes, as well as the global dynamics of natural processes in Ukraine, has not been sufficiently studied. The first step to solving it is to observe (monitor) changes in the number and distribution of species. Taking into account anthropogenic, climatic, etc. factors that pose a threat to biodiversity, the study of the state of fauna and flora, the study and conservation of species biodiversity is extremely relevant. The purpose of the work is to determine the state and structure of the entomological species biodiversity of the mixed biotopes of Kyiv Polissia. The site of the research was chosen mixed biotopes of the Kyiv Polissia, which are located in the Vyshgorod district of the Kyiv region, (territories near the settlements: Lyutizh, Demydiv, Lytvynivka, as well as monitoring sites of various nature: biotopes of pine, deciduous, mixed forests, floodplains of rivers: Irpin, Kizka, Lisovychka and others. Methods tested and recommended for field and laboratory research in entomology, plant protection, and ecology were used for accounting. An important aspect in research was the skills of collecting and analyzing primary information, assessing species richness and diversity, determining the level of dominance of individual species in the biocenosis, and assessing the age composition of organisms in populations. Collection and accounting of entomological biodiversity was carried out according to generally accepted methods once every 7–10 days at stationary sites. The level and structure of entomological biodiversity of mixed biotopes of Kyiv Polissia in 2022-2023 were studied. As a result of the monitoring and accounting of entomological biodiversity in mixed biotopes, 88 species of insects were found, belonging to 23 families from 8 orders in a systematic manner. The most numerous is the order of Hymenoptera, which includes 12 species from 5 families and Coleoptera - 36 species from 4 families, which is 49.6% of the total number of studied entomological biodiversity. An annotated list was compiled and an analysis of the current state of the entomofauna of mixed biotopes was carried out.