Ecology and functional potential of phyllosphere yeasts

Gouka, Linda; Raaijmakers, Jos M.; Cordovez, Viviane

doi:10.1016/j.tplants.2022.06.007

Cited by 25 publications

(15 citation statements)

References 134 publications

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“…Biosynthesis of rhodotorulic acid, a dihydroxamate siderophore, by Rhodotorula has been reported with antagonistic activity against B. cinerea [ 40 ]. This suggests that some plant yeasts use siderophores to solubilize iron in soils or as a competitive strategy against other microorganisms in the phyllosphere [ 41 ]. Siderophores can also bind other divalent or trivalent cations, such as Co, Cu, Zn, As, Cr, Pb, and Cd [ 42 ].…”

Section: Resultsmentioning

confidence: 99%

Mycobiota of Mexican Maize Landraces with Auxin-Producing Yeasts That Improve Plant Growth and Root Development

Ramos-Garza

Aguirre-Noyola

Bustamante-Brito

et al. 2023

Plants

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Compared to agrochemicals, bioinoculants based on plant microbiomes are a sustainable option for increasing crop yields and soil fertility. From the Mexican maize landrace “Raza cónico” (red and blue varieties), we identified yeasts and evaluated in vitro their ability to promote plant growth. Auxin production was detected from yeast isolates and confirmed using Arabidopsis thaliana plants. Inoculation tests were performed on maize, and morphological parameters were measured. Eighty-seven yeast strains were obtained (50 from blue corn and 37 from red corn). These were associated with three families of Ascomycota (Dothideaceae, Debaryomycetaceae, and Metschnikowiaceae) and five families of Basidiomycota (Sporidiobolaceae, Filobasidiaceae, Piskurozymaceae, Tremellaceae, and Rhynchogastremataceae), and, in turn, distributed in 10 genera (Clavispora, Rhodotorula, Papiliotrema, Candida, Suhomyces, Soliccocozyma, Saitozyma Holtermaniella, Naganishia, and Aeurobasidium). We identified strains that solubilized phosphate and produced siderophores, proteases, pectinases, and cellulases but did not produce amylases. Solicoccozyma sp. RY31, C. lusitaniae Y11, R. glutinis Y23, and Naganishia sp. Y52 produced auxins from L-Trp (11.9–52 µg/mL) and root exudates (1.3–22.5 µg/mL). Furthermore, they stimulated the root development of A. thaliana. Inoculation of auxin-producing yeasts caused a 1.5-fold increase in maize plant height, fresh weight, and root length compared to uninoculated controls. Overall, maize landraces harbor plant growth-promoting yeasts and have the potential for use as agricultural biofertilizers.

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Section: Resultsmentioning

confidence: 99%

Mycobiota of Mexican Maize Landraces with Auxin-Producing Yeasts That Improve Plant Growth and Root Development

Ramos-Garza

Aguirre-Noyola

Bustamante-Brito

et al. 2023

Plants

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“…Dioszegia , in the same family as Hannaella , was identified as a network hub in the phyllosphere (Agler et al 2016), and the closely related yeast Bullera has been a network hub of the soybean phyllosphere (Longley et al 2020). These yeasts are generally non-pathogenic, but their ecological role is poorly understood (Gouka et al 2022). The prokaryote hubs were also intriguing because cotton contained several Nitrososphaeraceae pOTUs, which likely are involved with ammonia-oxidizdation in soils (Reyes et al 2020).…”

Section: Discussionmentioning

confidence: 99%

Soybean and cotton spermosphere soil microbiome shows dominance of soil-borne copiotrophs

Olofintila

Noel

2023

Preprint

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The spermosphere is the transient, immediate zone of soil around imbibing and germinating seeds, rich in microbial activity. It represents a habitat where there is contact between seed-associated microbes and soil microbes, but is studied less compared to other plant habitats. Previous studies on spermosphere microbiology were primarily culture-based or did not sample the spermosphere soil as initially defined in space and time. Thus, the objectives of this study were to develop an efficient strategy to collect spermosphere soils around imbibing soybean and cotton in non-sterile soil and investigate factors contributing to changes in microbial communities. The method employed sufficiently collected spermosphere soil as initially defined in space (3-10 mm soil around a seed) by constraining the soil sampled with an cork borer and confining the soil to a 12-well microtiter plate. Spermosphere prokaryote composition changed over time and depended on the crop within six hours after seeds were sown. By 12 to 18 hours, crops had unique microbial communities in spermosphere soils. Prokaryote evenness dropped following seed imbibition and the proliferation of copiotroph soil bacteria in the phyla Proteobacteria and Firmicutes. Due to their long history of plant growth promotion, prokaryote OTUs in Bacillus, Paenibacillus, Burkholderia, Massilia, Azospirillum, and Pseudomonas were notable genera enriched in the cotton and soybean spermosphere. There was no consistent evidence that fungal communities changed like prokaryotes. However, fungi and prokaryotes were hub taxa in cotton and soybean spermosphere networks. Additionally, the enriched taxa were not hubs in networks, suggesting other taxa besides the copiotrophic may be important for spermosphere communities. Overall, this study advances knowledge in the assembly of the plant microbiome early in a plant's life, which may have plant health implications in more mature plant growth stages.

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“…Killer toxins, also called mycocins, are protein, glycoprotein, or glycolipids with a molecular weight of 10–30 kDa, produced for the competition with other environmental microorganisms and increasement of stress resistance . Toxin-producer yeast can compete for nutrients and space in the host fruit since the killer toxin secreted by the antagonistic yeast does not affect itself; it only has a lethal effect on other yeasts and molds. , The action mechanisms of killer toxins include disrupting the cell membrane, inhibiting cell wall and DNA synthesis, and blocking calcium uptake. ,, Mycocins are advantageous biocontrol mechanisms due to their nontoxicity to mammals, increased resistance to stress conditions, environmental friendliness, and decreased possibility of pathogen resistance . A study showed that the killer toxins of Debaryomyces hansenii strains were highly stable at pH 2.5–5.5 and 5–37 °C and could inhibit Alternaria brassicicola , Alternaria citri , Aspergillus niger , and Rhizopus stolonifer in fruits .…”

Section: Antagonistic Yeasts and Their Antifungal Mechanisms Of Actionmentioning

confidence: 99%

Recent Insights into the Use of Antagonistic Yeasts for Sustainable Biomanagement of Postharvest Pathogenic and Mycotoxigenic Fungi in Fruits with Their Prevention Strategies against Mycotoxins

Öztekin

Dikmetaş

Devecioglu

et al. 2023

J. Agric. Food Chem.

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Fungi-induced postharvest diseases are the leading causes of food loss and waste. In this context, fruit decay can be directly attributed to phytopathogenic and/or mycotoxin-producing fungi. The U.N. Sustainable Development Goals aim to end hunger by 2030 by improving food security, sustainable agriculture, and food production systems. Antagonistic yeasts are one of the methods presented to achieve these goals. Unlike physical and chemical methods, harnessing antagonistic yeasts as a biological method controls the decay caused by fungi and adsorbs and/or degrades mycotoxins sustainably. Therefore, antagonistic yeasts and their antifungal mechanisms have gained importance. Additionally, mycotoxins’ biodetoxification is carried out due to the occurrence of mycotoxin-producing fungal species in fruits. Combinations with processes and agents have been investigated to increase antagonistic yeasts’ efficiency. Therefore, this review provides a comprehensive summary of studies on preventing phytopathogenic and mycotoxigenic fungi and their mycotoxins in fruits, as well as biocontrolling and biodetoxification mechanisms.

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Ecology and functional potential of phyllosphere yeasts

Cited by 25 publications

References 134 publications

Mycobiota of Mexican Maize Landraces with Auxin-Producing Yeasts That Improve Plant Growth and Root Development

Mycobiota of Mexican Maize Landraces with Auxin-Producing Yeasts That Improve Plant Growth and Root Development

Soybean and cotton spermosphere soil microbiome shows dominance of soil-borne copiotrophs

Recent Insights into the Use of Antagonistic Yeasts for Sustainable Biomanagement of Postharvest Pathogenic and Mycotoxigenic Fungi in Fruits with Their Prevention Strategies against Mycotoxins

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